植物科学

分类

    现刊
    Visualization of Actin Organization and Quantification in Fixed Arabidopsis Pollen Grains and Tubes
    固定拟南芥花粉粒及花粉管中肌动蛋白组织可视化及定量分析
    作者:Xiaolu Qu, Qiannan Wang, Haiyan Wang and Shanjin Huang日期:01/05/2020,浏览量:2589,Q&A: 0
    [Abstract] Although it is widely accepted that actin plays an important role in regulating pollen germination and pollen tube growth, how actin exactly performs functions remains incompletely understood. As the function of actin is dictated by its spatial organization, it is the key to reveal how exactly actin distributes in space in pollen cells. Here we ...
    Analysis of Chromosome Condensation/Decondensation During Mitosis by EdU Incorporation in Nigella damascena L. Seedling Roots
    通过EdU掺入分析大马士革黑种草幼苗根中有丝分裂过程中染色体的凝集/解凝集
    作者:Eugene V. Sheval日期:02/05/2018,浏览量:4473,Q&A: 0
    [Abstract] To investigate the chromosome dynamics during mitosis, it is convenient to mark the discrete chromosome foci and then analyze their spatial rearrangements during prophase condensation and telophase decondensation. To label the chromosome regions in plant chromosomes, we incorporated the synthetic nucleotide, 5-ethynyl-2’-deoxyuridine (EdU), which ...
    Biomechanical Characterization of Onion Epidermal Cell Walls
    洋葱表皮细胞壁的生物力学表征
    作者:Daniel M. Durachko, Yong Bum Park, Tian Zhang and Daniel J. Cosgrove日期:12/20/2017,浏览量:9398,Q&A: 1
    [Abstract] Here we describe two experimental protocols to measure the biomechanical properties of primary (growing) plant cell walls, with a focus on analyzing cell wall epidermal strips of onion scales. The first protocol measures cell wall creep (time-dependent irreversible extension) under constant force. Such creep is often mediated by the wall-loosening ...
    Using Light and Electron Microscopy to Estimate Structural Variation in Thylakoid Membranes
    使用光学和电子显微镜估计类囊体膜的结构变异
    作者:Hatsumi Nozue and Kiyokazu Kametani日期:12/05/2017,浏览量:7237,Q&A: 0
    [Abstract] The shapes of chloroplasts and the architectures of internal thylakoid membranes are altered by growth and environmental changes (Lichtenthaler et al., 1981; Kutik, 1985; Terashima and Hikosaka, 1995). These morphological alterations proceed via transitional intermediates, during which dynamic and heterogeneous thylakoid membranes are ...
    Simple Methods for Screening and Statistical Analysis of Leaf Epidermal Cells in Dicotyledonous Plants
    双子叶植物中叶表皮细胞筛选和统计分析的简单方法
    作者:Jian-Jun Tao, Shou-Yi Chen and Jin-Song Zhang日期:09/05/2016,浏览量:10170,Q&A: 0
    [Abstract] Leaf epidermal cell size and number are positively correlated to leaf area. Stomata are specialized epidermal cells vital for gas exchange and water transpiration. So, observation and statistical analysis of the leaf epidermal cells are valuable for the study of leaf development and response to environmental stimulus. The classical method is using ...
    In vitro Microtubule Binding Assay and Dissociation Constant Estimation
    体外微管结合实验和解离常数估算
    作者:Christopher Kesten, René Schneider and Staffan Persson日期:03/20/2016,浏览量:12121,Q&A: 0
    [Abstract] Microtubules (MTs) support an astonishing set of versatile cellular functions ranging from cell division, vesicle transport, and cell and tissue morphogenesis in various organisms. This versatility is in large mediated by MT-associated proteins (MAPs). The neuronal MAP Tau, for example, is stabilizing MTs in axons of the vertebrate nervous system ...
    Super-resolution Imaging of Live BY2 Cells Using 3D-structured Illumination Microscopy
    采用三维结构照明显微镜对活的BY2细胞进行超分辨率成像
    作者:Karen Bell, Karl Oparka and Kirsten Knox日期:01/05/2016,浏览量:7471,Q&A: 0
    [Abstract] Light microscopy is the standard tool for studying sub-cellular structures however, owing to the diffractive properties of light, resolution is limited to 200 nm. Super-resolution microscopy methods circumvent this limit, offering greater resolution, particularly when studying fluorescently labeled sub-cellular structures. Super-resolution methods ...
    Observation of Chloroplast Movement in Vallisneria
    苦草属中叶绿体运动的观察
    作者:Yuuki Sakai and Shingo Takagi日期:11/05/2015,浏览量:7556,Q&A: 0
    [Abstract] Chloroplasts accumulate to weak light and escape from strong light. These light-induced responses have been known from the 19th century (Böhm, 1856). Up to now, many scientists have developed different methods to investigate these dynamic phenomena in a variety of plant species including the model plant Arabidopsis thaliana, a terrestrial ...
    Atomic Force Microscopy (AFM) Analysis of Cell Wall Structural Glycoproteins in vitro
    原子力显微镜(AFM)法体外分析细胞壁的结构糖蛋白
    作者:Yuning Chen, Liwei Chen, Marcia J. Kieliszewsk and Maura C. Cannon日期:07/20/2015,浏览量:7366,Q&A: 0
    [Abstract] Hydroxyproline-rich glycoproteins (HRGPs) are major protein components in dicot primary cell walls and generally account for more than 10% of the wall dry weight. As essential members of the HRGP superfamily, extensins (EXTs) presumably function in the cell wall by assembling into positively charged protein scaffolds (Cannon et al., 2008) ...
    Real-time Analysis of Lateral Root Organogenesis in Arabidopsis
    拟南芥侧根器官形成的实时分析
    作者:Peter Marhavý and Eva Benková日期:04/20/2015,浏览量:8116,Q&A: 0
    [Abstract] Plants maintain capacity to form new organs such as leaves, flowers, lateral shoots and roots throughout their postembryonic lifetime. Lateral roots (LRs) originate from a few pericycle cells that acquire attributes of founder cells (FCs), undergo series of anticlinal divisions, and give rise to a few short initial cells. After initiation, ...