分子生物学

分类

    现刊
    En masse DNA Electroporation for in vivo Transcriptional Assay in Ascidian Embryos
    海鞘胚胎中DNA电穿孔的体内转录分析
    作者:Sébastien Darras日期:09/20/2021,浏览量:946,Q&A: 0
    [Abstract]

    Ascidian embryos are powerful models for functional genomics, in particular, due to the ease of generating a large number of transgenic embryos by electroporation. In addition, the small size of their genome makes them an attractive model for studying cis-regulatory elements that control gene expression during embryonic development. Here, I

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    A Fast and Easy Method to Study Ralstonia solanacearum Virulence upon Transient Gene Expression or Gene Silencing in Nicotiana benthamiana Leaves
    利用本生烟叶片基因瞬时表达或沉默研究青枯菌毒力的快速简便方法
    作者:Wenjia Yu and Alberto P. Macho日期:08/05/2021,浏览量:1161,Q&A: 0
    [Abstract]

    Ralstonia solanacearum is a devastating soil-borne bacterial pathogen that causes disease in multiple host plants worldwide. Typical assays to measure virulence of R. solanacearum in laboratory conditions rely on soil-drenching inoculation followed by observation and scoring of disease symptoms. Here, we describe a novel inoculation protocol to

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    Cellulase and Macerozyme-PEG-mediated Transformation of Moss Protoplasts
    纤维素酶和离析酶-PEG介导的苔藓原生质体转化
    [Abstract] This protocol describes the generation of protoplasts from protonemal tissue of the moss Physcomitrium patens (syn. Physcomitrella patens), using Cellulase ONOZUKA R10 and Macerozyme R10, followed by polyethylene glycol (PEG) mediated transformation. The protonemal tissue grown in liquid suspension was harvested and treated with ...
    Agrobacterium-mediated Transformation of Sweet Basil (Ocimum basilicum)
    农杆菌介导的罗勒(Ocimum basilicum)转化
    作者:Natasha Navet and Miaoying Tian日期:11/20/2020,浏览量:2637,Q&A: 1
    [Abstract]

    Sweet basil (Ocimum basilicum) is a popular herb with high economic value and is currently threatened by a severe oomycete disease. An efficient transformation method is a prerequisite for gene functional analysis to accelerate molecular breeding and deploy effective disease management strategies, and breeding through genetic engineering. Here we

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    PEG-mediated, Stable, Nuclear and Chloroplast Transformation of Cyanidioschizon merolae
    PEG介导的稳定的Cyanidioschizon merolae细胞核和叶绿体转化
    作者:Maksymilian Zienkiewicz, Tomasz Krupnik, Anna Drożak and Kinga Kania日期:09/05/2019,浏览量:2760,Q&A: 0
    [Abstract] The ability to achieve nuclear or chloroplast transformation in plants has been a long standing goal, especially in microalgae research. Over past years there has been only little success, but transient and stable nuclear transformation has been achieved in multiple species. Our newly developed method allows for relatively simple transformation of ...
    Development of an Efficient Transformation System for Halotolerant Yeast Debaryomyces hansenii CBS767
    耐盐汉斯德巴氏酵母菌CBS767的一种高效转化体系的建立
    作者:Anu P. Minhas and Dipanwita Biswas日期:09/05/2019,浏览量:2224,Q&A: 0
    [Abstract] Debaryomyces hansenii is one of the most osmotolerant and halotolerant yeasts. Further, its association with traditional cheese and meat products imparting special flavors to these products project this yeast with enormous biotechnological potential in the agrofood sector. However, lack of an efficient transformation system in D. hansenii ...
    Measurement of the Length of the Integrated Donor DNA during Bacillus subtilis Natural Chromosomal Transformation
    枯草芽孢杆菌自然遗传转化过程中完整供体DNA长度的测定
    作者:Ester Serrano and Begoña Carrasco日期:08/20/2019,浏览量:2313,Q&A: 0
    [Abstract] For natural transformation to occur, bacterial cells must first develop a programmed physiological state called competence. Competence in Bacillus subtilis, which occurs only in a fraction of cells, is a transient stress response that allows cells to take up DNA from the environment. During natural chromosomal transformation, the ...
    Quantitative Transformation Efficiency Assay for Bacillus subtilis
    枯草芽孢杆菌转化效率的定量测定
    作者:Christian L. Loyo and Briana M. Burton日期:12/05/2018,浏览量:5770,Q&A: 0
    [Abstract] Bacillus subtilis (B. subtilis) is a model Gram-positive organism used to study a variety of physiological states and stress responses, one of which is the development of competence. Competence refers to the physiological state of a cell which allows it to be transformed naturally. Through induction of competence, the efficiency ...
    Induction of Natural Competence in Genetically-modified Lactococcus lactis
    转基因乳酸乳球菌自然感受态的诱导
    作者:Joyce Mulder, Michiel Wels, Oscar P. Kuipers, Michiel Kleerebezem and Peter A. Bron日期:07/05/2018,浏览量:5035,Q&A: 0
    [Abstract] Natural competence can be activated in Lactoccocus lactis subsp lactis and cremoris upon overexpression of ComX, a master regulator of bacterial competence. Herein, we demonstrate a method to activate bacterial competence by regulating the expression of the comX gene by using a nisin-inducible promoter in an L. ...
    Transient Gene Expression for the Characteristic Signal Sequences and the Estimation of the Localization of Target Protein in Plant Cell
    植物细胞中特征信号序列的瞬时基因表达及靶蛋白定位的估判
    [Abstract] We have proposed and tested a method for characterization of the signal sequences and determinations of target protein localization in a plant cell. This method, called the AgI-PrI, implies extraction of protoplasts from plant tissues after agroinfiltration. The suggested approach combines the advantages of two widely used methods for transient ...