干细胞

分类

    现刊
    Rapid and Simplified Induction of Neural Stem/Progenitor Cells (NSCs/NPCs) and Neurons from Human Induced Pluripotent Stem Cells (hiPSCs)
    从人诱导的多能干细胞(hiPSCs)快速简便地诱导神经干/祖细胞(NSCs/NPCs)和神经元
    作者:Ryutaro Kajihara, Tadahiro Numakawa and Takumi Era日期:02/05/2021,浏览量:2136,Q&A: 0
    [Abstract]

    Human induced pluripotent stem cells (iPSCs) and their progeny displaying tissue-specific characteristics have paved the way for regenerative medicine and research in various fields such as the elucidation of the pathological mechanism of diseases and the discovery of drug candidates. iPSC-derived neurons are particularly valuable as it is

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    Protocol for Isolation, Stimulation and Functional Profiling of Primary and iPSC-derived Human NK Cells
    原代和iPSC来源的人类NK细胞的分离、刺激和功能分析方案
    [Abstract]

    Natural killer (NK) cells are innate immune cells, characterized by their cytotoxic capacity, and chemokine and cytokine secretion upon activation. Human NK cells are identified by CD56 expression. Circulating NK cells can be further subdivided into the CD56bright (~10%) and CD56dim NK cell subsets (~90%). NK cell-like cells can also be derived

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    Generating Loss-of-function iPSC Lines with Combined CRISPR Indel Formation and Reprogramming from Human Fibroblasts
    人成纤维细胞通过CRISPR 插入缺失的形成联合重编程生成功能丧失iPSC细胞系
    作者:Andrew M. Tidball, Preethi Swaminathan, Louis T. Dang and Jack M. Parent日期:04/05/2018,浏览量:8777,Q&A: 1
    [Abstract] For both disease and basic science research, loss-of-function (LOF) mutations are vitally important. Herein, we provide a simple stream-lined protocol for generating LOF iPSC lines that circumvents the technical challenges of traditional gene-editing and cloning of established iPSC lines by combining the introduction of the CRISPR vector ...
    GC/MS-based Analysis of Volatile Metabolic Profile Along in vitro Differentiation of Human Induced Pluripotent Stem Cells
    基于GC/MS的人诱导多能干细胞体外分化过程中挥发性化合物代谢谱分析
    [Abstract] Human induced pluripotent stem cells (hiPSCs) are a promising tool in cell-based therapies for degenerative diseases. A safe application of hiPSCs in vivo, requires the detection of the presence of residual undifferentiated pluripotent cells that can potentially cause the insurgence of teratomas. Several studies point out that metabolic ...
    Exit from Pluripotency Assay of Mouse Embryonic Stem Cells
    小鼠胚胎干细胞退出多能性测定实验
    作者:Daniel Cirera-Salinas and Constance Ciaudo日期:08/20/2017,浏览量:6842,Q&A: 0
    [Abstract] A novel method to assess the dissolution of the core pluripotency transcription-factor circuit of mouse Embryonic Stem Cells (mESCs) has been developed (Ying et al., 2003; Betschinger et al., 2013). In order to efficiently identify genes essential for the break-down of the pluripotency network in mutant mESCs with proliferation ...
    Differentiation of Human Induced Pluripotent Stem Cells (iPS Cells) and Embryonic Stem Cells (ES Cells) into Dendritic Cell (DC) Subsets
    诱导的人多能干细胞(iPS细胞)和胚胎干细胞(ES细胞)向树突状细胞(DC)亚型的分化
    作者:Stephanie Sontag, Malrun Förster, Kristin Seré and Martin Zenke日期:08/05/2017,浏览量:11805,Q&A: 0
    [Abstract] Induced pluripotent stem cells (iPS cells) are engineered stem cells, which exhibit properties very similar to embryonic stem cells (ES cells; Takahashi and Yamanaka, 2016). Both iPS cells and ES cells have an extraordinary self-renewal capacity and can differentiate into all cell types of our body, including hematopoietic stem/progenitor cells ...
    Efficient Production of Functional Human NKT Cells from Induced Pluripotent Stem Cells − Reprogramming of Human Vα24+iNKT Cells
    诱导多能干细胞高效生成功能性人NKT细胞 - 人Vα24+iNKT细胞的重新编程
    作者:Daisuke Yamada, Tomonori Iyoda, Kanako Shimizu, Yusuke Sato, Haruhiko Koseki and Shin-ichiro Fujii日期:05/20/2017,浏览量:9531,Q&A: 0
    [Abstract] Antigen-specific T cell-derived induced pluripotent stem cells (iPSCs) have been shown to re-differentiate into functional T cells and thus provide a potential source of T cells that could be useful for cancer immunotherapy. Human Vα24+ invariant natural killer T (Vα24+iNKT) cells are subset of T cells that are characterized ...
    Reprogram Murine Epiblast Stem Cells by Epigenetic Inhibitors
    通过表观遗传抑制剂重新编程鼠外胚层干细胞
    作者:Hui Zhang and Yali Dou日期:03/05/2017,浏览量:5458,Q&A: 0
    [Abstract] Pluripotent stem cells in the naïve state are highly useful in regenerative medicine and tissue engineering. A robust reprogramming of the primed murine Epiblast Stem Cells (EpiSCs) to naïve pluripotency is feasible via chemical-only approach. This protocol described a method to reprogram murine EpiSCs by MM-401 treatment, which blocks histone ...
    iPS Cell Induction from Human Non-T, B cells from Peripheral Blood
    用人外周血液中的非T淋巴细胞、B细胞诱导生成全能干细胞
    作者:Keisuke Okita日期:09/20/2013,浏览量:7831,Q&A: 0
    [Abstract] The generation of iPS cells gives an opportunity to use patient-specific somatic cells which are a valuable source for disease modeling and drug discovery. To promote these studies, it is important to make iPS cells from easily accessible and less invasive tissues like blood. Here, we describe the basic method to generate human iPS cells from ...