细胞生物学

分类

    现刊
    Visualization and Quantitation of Wg trafficking in the Drosophila Wing Imaginal Epithelium
    果蝇翅成虫上皮中Wg运输的可视化和定量研究
    作者:Leonie Witte, Karen Linnemannstöns, Mona Honemann-Capito and Julia Christina Gross日期:06/05/2021,浏览量:299,Q&A: 0
    [Abstract]

    Secretory Wnt trafficking can be studied in the polarized epithelial monolayer of Drosophila wing imaginal discs (WID). In this tissue, Wg (Drosophila Wnt-I) is presented on the apical surface of its source cells before being internalized into the endosomal pathway. Long-range Wg secretion and spread depend on secondary secretion from endosomal

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    Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Protein Vesicle Tracking in HeLa Cells
    用RUSH蛋白分选法追踪HeLa细胞中的蛋白囊泡
    作者:Natalia Pacheco-Fernandez, Mehrshad Pakdel and Julia von Blume日期:03/05/2021,浏览量:1543,Q&A: 0
    [Abstract]

    Monitoring vesicle trafficking is an excellent tool for the evaluation of protein dynamics in living cells. Such study is key for the understanding of protein sorting and secretion. Recent developments in microscopy, as well as new methodologies developed to study synchronized trafficking of proteins, allowed a better understanding of signaling,

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    Immuno-electrophysiology on Neuromuscular Junctions of Drosophila Third Instar Larva
    果蝇三龄幼虫神经肌肉连接的免疫电生理研究
    作者:Raffaella Klima, Giulia Romano, Monsurat Gbadamosi, Aram Megighian and Fabian Feiguin日期:02/05/2021,浏览量:670,Q&A: 0
    [Abstract]

    Alterations in synaptic transmission are critical early events in neuromuscular disorders. However, reliable methodologies to analyze the functional organization of the neuromuscular synapses are still needed. This manuscript provides a detailed protocol to analyze the molecular assembly of the neuromuscular synapses through

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    Optimized Immunostaining of Embryonic and Early Postnatal Mouse Brain Sections
    胚胎和产后早期小鼠脑切片的优化免疫染色
    作者:Kawssar Harb, Michele Bertacchi and Michèle Studer日期:01/05/2021,浏览量:1356,Q&A: 0
    [Abstract]

    The mammalian neocortex, the outer layer of the cerebrum and most recently evolved brain region, is characterized by its unique areal and laminar organization. Distinct cortical layers and areas can be identified by the protein expression of graded transcription factors and molecular determinants that define the identity of different projection

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    Use of Optogenetic Amyloid-β to Monitor Protein Aggregation in Drosophila melanogaster, Danio rerio and Caenorhabditis elegans
    利用光遗传学淀粉样蛋白-β监测黑腹果蝇、斑马鱼和秀丽隐杆线虫体内蛋白质聚集
    作者:Prameet Kaur, Caroline Kibat, Emelyne Teo, Jan Gruber, Ajay Mathuru and Nicholas S. Tolwinski日期:12/05/2020,浏览量:1544,Q&A: 0
    [Abstract]

    Alzheimer’s Disease (AD) has long been associated with accumulation of extracellular amyloid plaques (Aβ) originating from the Amyloid Precursor Protein. Plaques have, however, been discovered in healthy individuals and not all AD brains show plaques, suggesting that extracellular Aβ aggregates may play a smaller role than

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    In vitro Time-lapse Imaging of Primary Cilium in Migrating Neuroblasts
    迁移神经母细胞中初级纤毛的体外延时成像
    作者:Masato Sawada, Mami Matsumoto, Keishi Narita, Natsuko Kumamoto, Shinya Ugawa, Sen Takeda and Kazunobu Sawamoto日期:11/20/2020,浏览量:1144,Q&A: 0
    [Abstract] Neuronal migration is a critical step for the development of neuronal circuits in the brain. Immature new neurons (neuroblasts) generated in the postnatal ventricular-subventricular zone (V-SVZ) show a remarkable potential to migrate for a long distance at a high speed in the postnatal mammalian brain, and are thus a powerful model to analyze the ...
    Real-time Three-dimensional Tracking of Endocytic Vesicles
    内囊泡的实时三维追踪
    作者:Stephanie Duhamel and Kossay Zaoui日期:10/20/2020,浏览量:1330,Q&A: 0
    [Abstract] Endocytic trafficking and recycling are fundamental cellular processes that control essential functions such as signaling protein complexes transport and membrane identity. The small GTPase Rabs are indispensable component of the endosomal recycling machinery. The Rabs bind to effectors to mediate their functions, such as protein sorting and ...
    Co-immunostaining of ICAM-1, ICAM-2, and CD31 in Mouse Kidney Glomeruli
    小鼠肾小球ICAM-1、ICAM-2和CD31的联合免疫染色
    作者:Sun-sang J. Sung日期:07/05/2020,浏览量:1653,Q&A: 0
    [Abstract] Glomerulonephritis (GN) is a common pathological condition in chronic kidney diseases that often leads to end stage renal failure. Mac-1 (CD11b/CD18)-mediated neutrophil, macrophage, and dendritic cell glomerular infiltration leading to cellular dysfunction and destruction is an important disease mechanism. The cellular distribution and dynamics ...
    Maintenance and Quantitative Phenotyping of the Oomycete-plant Model Pathosystem Hyaloperonospora arabidopsidisArabidopsis
    卵菌-植物模型病理系统(活体营养型卵菌–拟南芥)的维持和定量表型分析
    作者:Fang-Yu Hwu and Martin Parniske日期:06/20/2020,浏览量:1752,Q&A: 0
    [Abstract] The interaction between the host plant Arabidopsis thaliana (Arabidopsis) and the oomycete Hyaloperonospora arabidopsidis (Hpa) is an established model system for the study of an obligate biotrophic downy mildew interaction. The evaluation of the developmental success of Hpa is often based on the quantification ...
    Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration
    神经退行性细胞模型中核质转运的定量分析
    作者:Joni Vanneste, Thomas Vercruysse, Philip Van Damme, Ludo Van Den Bosch and Dirk Daelemans日期:06/20/2020,浏览量:2029,Q&A: 0
    [Abstract] Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and which pathways are involved is of great importance. Here, we make use of CRISPR-Cas9 technology to ...