细胞生物学

分类

    现刊
    Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISH
    利用显色原位杂交进行固定细胞和组织中单个RNA的检测
    作者:Meng Jiang, Chen Lin and Rongqin Ke日期:02/05/2020,浏览量:216,Q&A: 0
    [Abstract] Visualization of RNA molecules in situ helps to better understand the functions of expressed genes. Currently, most conventional in situ hybridization methods for visualization of individual RNAs are based on fluorescence detection. Herein we present a chromogenic in situ hybridization protocol for visualization of ...
    Retina Injury and Retina Tissue Preparation to Study Regeneration in Zebrafish
    通过斑马鱼视网膜损伤和视网膜组织制备进行视力恢复研究
    作者:Poonam Sharma and Rajesh Ramachandran日期:12/20/2019,浏览量:483,Q&A: 0
    [Abstract] Unlike mammals, primitive vertebrates have immense capability to regenerate almost all of their organs including the central nervous system. Among primitive organisms, zebrafish have been extensively used as a model system for regeneration studies. The retina is a part of the central nervous system and mammals lack the potential to repair any ...
    Analysis of EGF Receptor Endocytosis Using Fluorogen Activating Protein Tagged Receptor
    利用荧光团激活蛋白标记受体分析EGF受体的内吞作用
    作者:Mireia Perez Verdaguer, Mads B. Larsen, Marcel P. Bruchez, Simon C. Watkins and Alexander Sorkin日期:12/20/2019,浏览量:541,Q&A: 0
    [Abstract] Functional activities of many transmembrane proteins are controlled by their endocytosis. One of the most studied experimental models is the epidermal growth factor (EGF) receptor (EGFR). However, endocytic trafficking of EGFR has been predominantly analyzed using labeled EGF, whereas quantitative analyses of the endocytosis of the receptor itself ...
    Determination of Chromatin Accessibility in Drosophila Midgut Enterocytes by in situ 5mC Labeling
    利用原位5mC标记进行果蝇中肠细胞染色质开放性的鉴定
    作者:Eliya Bitman-Lotan, Hector Rincon-Arano, Gal Raz and Amir Orian日期:11/20/2019,浏览量:548,Q&A: 0
    [Abstract] Regulation of gene expression involves dynamic changes in chromatin organization, where in many cases open chromatin structure correlates with gene activation. Several methods enable monitoring changes in chromatin accessibility, including ATAC-seq, FAIRE-seq, MNase-seq and DNAse-seq methods, which involve Next-generation-sequencing (NGS). ...
    Leishmania Parasite Quantification by Bioluminescence in Murine Models
    在小鼠模型中利用生物发光进行利什曼原虫的定量分析
    作者:Marta Reverte and Nicolas Fasel日期:11/20/2019,浏览量:398,Q&A: 0
    [Abstract] Leishmaniasis remains a major public health problem worldwide with a prevalence of 12 million, an incidence of 1 million persons, and 350 million people being at risk. Murine models have been largely used for studying the host-pathogen relationship and developing effective chemotherapies against Leishmania parasites. Thus, preclinical ...
    Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay
    果蝇翅膀成虫盘体内培养和弗林蛋白酶抑制剂检测
    作者:Alex Sohr, Lijuan Du and Sougata Roy日期:08/20/2019,浏览量:867,Q&A: 0
    [Abstract] Furin is an evolutionarily conserved proprotein convertase (PC) family enzyme with a broad range of substrates that are essential for developmental, homeostatic, and disease pathways. Classical genetic approaches and in vitro biochemical or cell biological assays identified that precursor forms of most growth factor family proteins are ...
    A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning
    利用改进的Sca/eS和机器学习用于螺旋器中毛细胞的成像和分析
    [Abstract] Here, we describe a sorbitol-based optical clearing method, called modified Sca/eS that can be used to image all hair cells (HCs) in the mouse cochlea. This modification of Sca/eS is defined by three steps: decalcification, de-lipidation, and refractive index matching, which can all be completed within 72 h. Furthermore, we established automated ...
    Characterization of Mouse Adult Testicular Macrophage Populations by Immunofluorescence Imaging and Flow Cytometry
    免疫荧光成像和流式细胞术鉴定小鼠成人睾丸巨噬细胞群体
    作者:Noushin Mossadegh-Keller and Michael H. Sieweke日期:03/05/2019,浏览量:1680,Q&A: 0
    [Abstract] Testicular macrophages (tMΦ) are the most abundant immune cells residing in the testis, an immune-privileged organ. TMΦ are known to exhibit different functions, such as protecting spermatozoa from auto-immune attack by producing immunosuppressive cytokines and trophic roles in supporting spermatogenesis and male sex hormone production. They also ...
    Blinded Visual Scoring of Images Using the Freely-available Software Blinder
    使用免费软件Blinder对图像进行盲视觉评分
    作者:Steven D. Cothren, Joel N. Meyer and Jessica H. Hartman日期:12/05/2018,浏览量:1992,Q&A: 0
    [Abstract] In nearly all subfields of biomedical sciences, there are phenotypes that are currently classified by expert visual scoring. In research applications, these classifications require the experimenter to be blinded to the treatment group in order to avoid unintentional bias in scoring. Currently, many labs either use laborious and tedious methods to ...
    Staining the Germline in Live Caenorhabditis elegans: Overcoming Challenges by Applying a Fluorescent-dye Feeding Strategy
    利用荧光染料喂食的方法活体染色标记秀丽隐杆线虫生殖腺
    作者:K. Adam Bohnert日期:11/05/2018,浏览量:2456,Q&A: 0
    [Abstract] C. elegans provides a tractable model organism for studying germline cell biology. Microscopy experiments are relatively facile, as this worm is transparent and germline development can be observed in real-time using DIC microscopy and/or fluorescent transgenes. Despite these many tools, robust staining techniques for imaging germ cells ...