细胞生物学

分类

    现刊
    Membrane Flotation Assay
    膜漂浮分析
    作者:Dorothee A Vogt and Melanie Ott日期:04/05/2015,浏览量:11641,Q&A: 0
    [Abstract] Many postitive-stranded RNA viruses, such as Hepatitis C virus (HCV), highjack cellular membranes, including the Golgi, ER, mitchondria, lipid droplets, and utilize them for replication of their RNA genome or assembly of new virions. By investigating how viral proteins associate with cellular membranes we will better understand the roles of ...
    Isolation of Tomato Fruit Chromoplasts and Determination of ATP Levels
    番茄果实有色体的分离和ATP含量的测定
    [Abstract] It has recently been reported that tomato fruit chromoplasts can synthesize ATP de novo using an ATP synthase complex harboring an atypical γ-subunit which is also present in a variety of plant species. However many aspects related with the biochemical processes underlying this process remain largely unknown. Here we describe detailed ...
    Small-scale Subcellular Fractionation with Sucrose Step Gradient
    蔗糖不连续密度梯度离心法进行小规模亚细胞分级分离
    作者:Yuzuru Taguchi and Hermann M. Schätzl日期:06/05/2014,浏览量:14506,Q&A: 0
    [Abstract] Here, we introduce the protocol for small-scale and simple subcellular fractionation used in our recent publication (Taguchi et al., 2013), which uses homogenization by passing through needles and sucrose step-gradient.

    Subcellular fractionation is a very useful technique but usually a large number of cells are required. ...
    Cell Fractionation of Pseudomonas aeruginosa
    绿脓杆菌的细胞分级分离
    作者:Esteban Paredes-Osses and Kim R. Hardie日期:10/05/2013,浏览量:11081,Q&A: 1
    [Abstract] Pseudomonas aeruginosa is a Gram negative bacterium. Separating the cell envelope compartments enables proteins to be localized to confirm where in the cell they function. Cell fractionation can also provide a first step in a protein purification strategy (Williams et al., 1998). This protocol has been designed to obtain the ...
    Preparation of Pre- and Post-synaptic Density Fraction from Mouse Cortex
    从小鼠皮质中制备突触前和突出后的致密区
    作者:Chengyong Shen and Yong-Jun Chen日期:09/05/2013,浏览量:12651,Q&A: 1
    [Abstract] The understanding of the organization of postsynaptic signaling systems at excitatory synapses has been aided by the identification of proteins in the postsynaptic density (PSD) fraction, a subcellular fraction enriched in structures with the morphology of PSDs. Here we described an efficient way to isolate the crude synaptosome, presynaptic ...
    Isolation of Growth Cones from Mouse Brain
    从小鼠脑部分离神经元生长锥
    作者:Iryna Leshchyns’ka and Vladimir Sytnyk日期:08/05/2013,浏览量:9826,Q&A: 0
    [Abstract] Growth cones are motile structures at the tips of growing neurites, which play an essential role in regulation of growth and navigation of growing axons and dendrites of neurons in the developing nervous system. This protocol describes isolation of growth cones from the brain tissue from young mice. Growth cones isolated using this protocol have ...
    Subcellular Fractionation of Mouse Brain Homogenates
    小鼠脑组织匀浆的亚细胞分离
    作者:Ditte Olsen and Camilla Gustafsen日期:08/05/2013,浏览量:11951,Q&A: 0
    [Abstract] This subcellular fractionation protocol is used for separation of cellular organelles based on their density. We have designed and optimized the protocol for separation of subcellular compartments of brain homogenates with focus on the localization and trafficking of transmembrane proteins, but we have also successfully used this protocol for ...
    Subcellular Fractionation of Cultured Human Cell Lines
    人源细胞系的亚细胞分离
    作者:Zhuoyou Yu, Zhiguang Huang and Maria Li Lung日期:05/05/2013,浏览量:43114,Q&A: 6
    [Abstract] Subcellular localization is crucial for the proper functioning of a protein. Deregulation of subcellular localization may lead to pathological consequences and result in diseases like cancer. Immuno-fluorescent staining and subcellular fractionation can be used to determine localization of a protein. Here we discuss a protocol to separate the ...