分子生物学

分类

    现刊
    Estimation of the Minimum Number of Replication Origins Per Chromosome in any Organism
    任何生物体内每个染色体的最小复制起源点数目的估计
    作者:Marcelo S. da Silva日期:10/20/2020,浏览量:1462,Q&A: 0
    [Abstract] Eukaryote nuclear genomes predominantly replicate through multiple replication origins. The number of replication origins activated per chromosome during the S-phase duration may vary according to many factors, but the predominant one is replication stress. Several studies have applied different approaches to estimate the number and map the ...
    Nonenzymatic RNA-templated Synthesis of N3′→P5′ Phosphoramidate DNA
    N3'→P5'磷酰胺DNA的非酶RNA模板合成
    作者:Derek K. O'Flaherty, Lijun Zhou and Jack W. Szostak日期:09/05/2020,浏览量:1964,Q&A: 0
    [Abstract] The RNA world hypothesis describes a scenario where early life forms relied on RNA to govern both inheritance and catalyze useful chemical reactions. Prior to the emergence of enzymes capable of replicating the RNA genome, a nonenzymatic replication process would have been necessary to initiate Darwinian Evolution. However, the one-pot ...
    Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA
    通过合成共价闭合环状(3Cs)DNA构建无偏和专用CRISPR / Cas gRNA文库
    作者:Martin Wegner, Koraljka Husnjak and Manuel Kaulich日期:01/05/2020,浏览量:4655,Q&A: 0
    [Abstract] Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus there is a growing need for unbiased and tailored CRISPR/Cas gRNA libraries. Conventional methods for gRNA library generation apply PCR and cloning ...
    Enhanced-ice-COLD-PCR for the Sensitive Detection of Rare DNA Methylation Patterns in Liquid Biopsies
    Enhanced-ice-COLD-PCR用于液体活检中罕见DNA甲基化模式的敏感检测
    作者:Florence Mauger and Jörg Tost日期:12/05/2019,浏览量:2812,Q&A: 0
    [Abstract] In the context of precision medicine, the identification of novel biomarkers for the diagnosis of disease, prognosis, predicting treatment outcome and monitoring of treatment success is of great importance. The analysis of methylated circulating-cell free DNA provides great promise to complement or replace genetic markers for these applications, ...
    In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
    真核先导链/后随链DNA复制的体外分析
    作者:Grant Schauer, Jeff Finkelstein and Mike O’Donnell日期:09/20/2017,浏览量:7037,Q&A: 0
    [Abstract] The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, respectively, along with helicases, polymerase α-primase, DNA sliding clamps, clamp loaders and many other ...
    EdU Based DNA Synthesis and Cell Proliferation Assay in Maize Infected by the Smut Fungus Ustilago maydis
    在玉蜀黎黑粉菌感染玉米中进行基于EdU的DNA合成和细胞增殖实验
    作者:Amey Redkar and Gunther Doehlemann日期:03/20/2016,浏览量:7653,Q&A: 0
    [Abstract] The basidiomycetous smut fungus Ustilago maydis (U. maydis) infects all aerial parts of its host plant maize (Zea mays L.). Infection is seen in the form of prominent tumorous symptoms after the establishment of a biotrophic interaction with the host, usually around 5-6 days after infection. The fungus colonizes the ...
    In vitro DNA Polymerization Activity Assay Using Cell-free Extracts
    采用细胞抽提液进行体外DNA聚合活性测试
    作者:Anurag K. Sinha and Malay K. Ray日期:08/20/2014,浏览量:7318,Q&A: 0
    [Abstract] This protocol has been designed to measure the in-vitro DNA polymerization activity in crude cell extracts of the Antarctic bacterium Pseudomonas syrinagae Lz4W. This bacterium can grow at 4 °C with optimum growth rate at 22 °C. The slow growth rate of the bacterium observed at low temperature (4 °C) compared to higher ...
    EdU labeling of Trypanosome Cells and Their Kinetoplast DNA (kDNA)
    EdU标记锥体虫细胞及其动质体DNA(kDNA)
    作者:Jianyang Wang日期:06/20/2013,浏览量:8622,Q&A: 0
    [Abstract] Trypanosome mitochondrial genome, known as Kinetoplast DNA (kDNA), is a massive network of interlocked DNA rings. The studies of kDNA replication and architecture are of major significance since kDNA is a valid drug target. However, DNA in procyclic trypanosomes can not be labeled with tracer concentrations of 3[H]-thymidine, possibly ...