分子生物学

分类

    现刊
    A Sensitive and Specific PCR-based Assay to Quantify Hepatitis B Virus Covalently Closed Circular (ccc) DNA while Preserving Cellular DNA
    一种在保留细胞DNA的同时定量检测乙型肝炎病毒共价闭合环状(ccc)DNA的灵敏、特异PCR方法
    作者:Benno Zehnder, Stephan Urban and Thomas Tu日期:04/20/2021,浏览量:2637,Q&A: 0
    [Abstract]

    Hepatitis B virus (HBV) is the major cause of liver diseases and liver cancer worldwide. After infecting hepatocytes, the virus establishes a stable episome (covalently closed circular DNA, or cccDNA) that serves as the template for all viral transcripts. Specific and accurate quantification of cccDNA is difficult because infected cells contain

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    Real-time Base Excision Repair Assay to Measure the Activity of the 8-oxoguanine DNA Glycosylase 1 in Isolated Mitochondria of Human Skin Fibroblasts
    实时碱基切除修复法测定人皮肤纤维细胞的分离线粒体中8-氧鸟嘌呤DNA糖基化酶1的活性
    作者:Daniel Schniertshauer, Daniel Gebhard and Jörg Bergemann日期:03/20/2021,浏览量:1702,Q&A: 0
    [Abstract]

    7,8-dihydro-8-oxoguanine (8-oxoG) is one of the most common and mutagenic oxidative DNA damages induced by reactive oxygen species (ROS). Since ROS is mainly produced in the inner membranes of the mitochondria, these organelles and especially the mitochondrial DNA (mtDNA) contained therein are particularly affected by this damage. Insufficient

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    SMART (Single Molecule Analysis of Resection Tracks) Technique for Assessing DNA end-Resection in Response to DNA Damage
    SMART(切除轨迹单分子分析)技术用于评估DNA末端切除对DNA损伤的反应
    作者:Angela Altieri, Milena Dell’Aquila, Francesca Pentimalli, Antonio Giordano and Alfano Luigi日期:08/05/2020,浏览量:2262,Q&A: 0
    [Abstract] DNA double strand breaks (DSBs) are among the most toxic lesions affecting genome integrity. DSBs are mainly repaired through non-homologous end joining (NHEJ) and homologous recombination (HR). A crucial step of the HR process is the generation, through DNA end-resection, of a long 3′ single-strand DNA stretch, necessary to prime DNA synthesis ...
    Evaluation of the Efficiency of Genome Editing Tools by a Frameshift Fluorescence Protein Reporter
    通过移码荧光蛋白报告基因评估基因组编辑工具的效率
    作者:Balaji T. Moorthy, Akhilesh Kumar, Lauren X. Lotenfoe and Fangliang Zhang日期:05/20/2020,浏览量:2306,Q&A: 0
    [Abstract] In the last decade, genome editing has been the center of attention as a novel tool for mechanistic investigations and for potential clinical applications. Various genome editing tools like meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector-based nucleases (TALEN), and the clustered regularly interspaced short ...
    Ex vivo Analysis of DNA Repair Capacity of Human Peripheral Blood Mononuclear Cells by a Modified Host Cell Reactivation Assay
    利用改进的宿主细胞再活化反应进行人外周血单核细胞的DNA修复能力的离体检测
    作者:Katja Matt and Jörg Bergemann日期:08/05/2019,浏览量:2408,Q&A: 0
    [Abstract] The ability of humans to repair DNA damages decreases with increasing age. In order to be able to repair daily occurring DNA damages, it becomes more and more important to preserve repair capability of cells with aging. The preservation of DNA repair processes contributes to preventing DNA mutations and subsequently the onset of age-related ...
    Viral Chromosome Conformation Capture (V3C) Assays for Identifying Trans-interaction Sites between Lytic Viruses and the Cellular Genome
    病毒染色体构象俘获技术用于裂解病毒和细胞基因组之间反向相互作用位点的检测
    作者:Kinjal Majumder, Maria Boftsi and David J Pintel日期:03/20/2019,浏览量:2990,Q&A: 0
    [Abstract] The folding mechanisms of the mammalian genome package our genetic material into the nucleus, and in doing so, dictate its appropriate replication and expression. Chromosome conformation capture technology has enabled the dissection of the folding principles of the cellular genome. This has led to a better understanding of the role played by ...
    Microbial Mutagenicity Assay: Ames Test
    微生物诱变分析:Ames试验
    作者:Urvashi Vijay, Sonal Gupta, Priyanka Mathur, Prashanth Suravajhala and Pradeep Bhatnagar日期:03/20/2018,浏览量:26473,Q&A: 2
    [Abstract] The Microbial mutagenicity Ames test is a bacterial bioassay accomplished in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. While Ames test is used to identify the revert mutations which are present in strains, it can also be used to detect the mutagenicity of environmental samples such as drugs, dyes, ...
    Determination of DNA Damage in the Retina Photoreceptors of Drosophila
    果蝇视网膜光感受器DNA损伤测定
    作者:Damulewicz Milena and Pyza Elzbieta日期:02/05/2018,浏览量:4787,Q&A: 0
    [Abstract] The retina is sensitive for light damages, because of direct light exposure, especially intense blue and UV light, which increase level of ROS and other toxic phototransduction products in photoreceptor cells. In our previous work (Damulewicz et al., 2017a and 2017b), we used 8-oxo-deoxyguanosine (8-OHdG) as a marker for oxidative stress ...
    In vitro Assay to Measure DNA Polymerase β Nucleotide Insertion Coupled with the DNA Ligation Reaction during Base Excision Repair
    体外实验检测在碱基去除修复中聚合酶β联合DNA连接反应介导的核苷酸插入
    作者:Melike Çağlayan and Samuel H. Wilson日期:06/20/2017,浏览量:5973,Q&A: 0
    [Abstract] We previously reported that oxidized nucleotide insertion by DNA polymerase β (pol β) can confound the DNA ligation step during base excision repair (BER) (Çağlayan et al., 2017). Here, we describe a method to investigate pol β nucleotide insertion coupled with DNA ligation, in the same reaction mixture including dGTP or 8-oxo-dGTP, pol β ...
    Targeted Nucleotide Substitution in Mammalian Cell by Target-AID
    在哺乳动物细胞中通过靶向性AID进行核苷酸靶向置换
    作者:Takayuki Arazoe, Keiji Nishida and Akihiko Kondo日期:06/05/2017,浏览量:12552,Q&A: 0
    [Abstract] Programmable RNA-guided nucleases based on CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated protein) systems have been applied to various type of cells as powerful genome editing tools. By using activation-induced cytidine deaminase (AID) in place of the nuclease activity of the CRISPR/Cas9 system, we have ...