生物化学

分类

    现刊
    In vitro Assay for Bacterial Membrane Protein Integration into Proteoliposomes
    细菌膜蛋白在蛋白质脂质体中的体外整合
    作者:Hanako Nishikawa, Masaru Sasaki and Ken-ichi Nishiyama日期:05/20/2020,浏览量:489,Q&A: 0
    [Abstract] It is important to experimentally determine how membrane proteins are integrated into biomembranes to unveil the roles of the integration factors, and to understand the functions and structures of membrane proteins. We have developed a reconstitution system for membrane protein integration in E. coli using purified factors, in which the ...
    Measurement of Protein-Protein Interactions through Microscale Thermophoresis (MST)
    利用微量热泳动仪 (MST)检测蛋白间相互作用
    作者:Magnez Romain, Bryan Thiroux, Morgane Tardy, Bruno Quesnel and Xavier Thuru日期:04/05/2020,浏览量:511,Q&A: 0
    [Abstract] The binding interactions of PD-1 and PD-L1 have been studied by surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) over the past few years, but these investigations resulted in controversy regarding the values of the dissociation constant (Kd) (Freeman et al., 2000). MST is a powerful new method for the ...
    Contemporaneous Measurement of Outer and Inner Membrane Permeability in Gram-negative Bacteria
    革兰阴性菌中内外膜通透性的同期测定
    作者:Bo Ma, Chao Fang, Jing Zhang, Mingzhi Wang, Xiaoxing Luo and Zheng Hou日期:03/05/2020,浏览量:481,Q&A: 0
    [Abstract] The emergence and rapid spread of multidrug resistance in bacteria have led to the urgent need for novel antibacterial agents. Membrane permeabilization is the mechanism for many antibacterial molecules that are being developed against gram-negative bacteria. Thus, to determine the efficacy of a potential antibacterial molecule, it is important to ...
    Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation
    共免疫沉淀法评价哺乳动物核蛋白的自身相互作用
    作者:Claudia Cattoglio, Iryna Pustova, Xavier Darzacq, Robert Tjian and Anders S. Hansen日期:02/20/2020,浏览量:896,Q&A: 0
    [Abstract] Protein-protein interactions constitute the molecular foundations of virtually all biological processes. Co-immunoprecipitation (CoIP) experiments are probably the most widely used method to probe both heterotypic and homotypic protein-protein interactions. Recent advances in super-resolution microscopy have revealed that several nuclear proteins ...
    Photoactivable Cholesterol as a Tool to Study Interaction of Influenza Virus Hemagglutinin with Cholesterol
    利用光活化胆固醇研究流感病毒血凝素与胆固醇相互作用
    作者:Bodan Hu, Mohamed Rasheed Gadalla, Christoph Thiele and Michael Veit日期:02/20/2020,浏览量:704,Q&A: 0
    [Abstract] Non-covalent binding of cholesterol to the transmembrane region of proteins affect their functionalities, but methods to prove such an interaction are rare. We describe our protocol to label the hemagglutinin (HA) of Influenza virus with a cholesterol derivative in living cells or with immunoprecipitated protein. We synthesized a “clickable” ...
    Proximity Ligation Assay for the Investigation of the Intramolecular Interaction of ELMO1
    邻位连接技术用于ELMO1分子内相互作用研究
    作者:Wai Wa Ray Chan, Dik Long Dennis Chau, Wen Li and Kwok-Fai Lau日期:12/05/2019,浏览量:982,Q&A: 0
    [Abstract] Intramolecular interaction is a common mechanism that regulates protein activities. Conventionally, such interactions are investigated by classical in vitro biochemical assays. Here, we describe a protocol for studying the intramolecular interaction of cell motility and engulfment 1 (ELMO1) in mammalian cells by using proximity ligation ...
    Detection of in vivo Protein Interactions in All Bacterial Compartments by Förster Resonance Energy Transfer with the Superfolder mTurquoise2 ox-mNeongreen FRET Pair
    利用超折叠mTurquoise2 ox-mNeongreen荧光分子共振能量转移法检测细菌体内蛋白相互作用
    作者:Nils Y. Meiresonne, Elisa Consoli, Laureen M.Y. Mertens and Tanneke den Blaauwen日期:12/05/2019,浏览量:1079,Q&A: 0
    [Abstract] This protocol was developed to qualitatively and quantitatively detect protein-protein interactions in all compartments of Escherichia coli by Förster Resonance Energy Transfer (FRET) using the Superfolder mTurquoise2 ox-mNeonGreen FRET pair (sfTq2ox-mNG). This FRET pair has more than twice the detection range for FRET ...
    Measuring Small-molecule Inhibition of Protein Interactions in Live Cells Using FLIM-FRET
    利用FLIM-FRET进行小分子抑制蛋白相互作用的活细胞检测
    作者:James M. Pemberton, Qian Liu and David W. Andrews日期:10/20/2019,浏览量:2366,Q&A: 0
    [Abstract] This protocol was designed to quantitatively measure small-molecule displacement of proteins in live mammalian cells using fluorescence lifetime imaging microscopy–Förster resonance energy transfer (FLIM-FRET). Tumour cell survival is often dependent on anti-apoptotic proteins, which bind to and inhibit pro-apoptotic proteins, thus preventing ...
    Supported Cell Membrane Sheets to Monitor Protein Assembly
    蛋白质组装的细胞膜片监测
    作者:Simon Erlendsson, Thor Seneca Thorsen and Kenneth Lindegaard Madsen日期:09/20/2019,浏览量:1187,Q&A: 0
    [Abstract] Studying protein-protein and protein-lipid interactions in their native environment is highly desirable, yet, the heterogeneity and complexity of cellular systems limits the repertoire of experimental methods available. In cells, interactions are often taking place in confined microenvironments where factors such as avidity, hindered diffusion, ...
    Cell-based Assay for Recruitment of DDR1 to Collagen-coated Beads
    以细胞为基础的DDR1向胶原包被微珠的招募分析
    作者:Victoria Juskaite and Birgit Leitinger日期:08/20/2019,浏览量:1162,Q&A: 0
    [Abstract] The discoidin domain receptors, DDR1 and DDR2, are key signaling receptors for the extracellular matrix protein collagen. The interactions of cells with collagen are difficult to study because of the difficulty to obtain native collagen fibers for in vitro studies. Thus, in vitro studies often use acid-soluble collagens in the ...