微生物学

分类

    现刊
    Isolation and Characterization of Membrane Vesicles from Lactobacillus Species
    乳酸杆菌属膜囊泡中的分离和特性研究
    作者:Julie C. Caruana, Scott N. Dean and Scott A. Walper日期:09/05/2021,浏览量:764,Q&A: 0
    [Abstract]

    Throughout their life cycle, bacteria shed portions of their outermost membrane comprised of proteins, lipids, and a diversity of other biomolecules. These biological nanoparticles have been shown to have a range of highly diverse biological activities, including pathogenesis, community regulation, and cellular defense (among others). In recent

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    A Yeast Chromatin-enriched Fractions Purification Approach, yChEFs, from Saccharomyces cerevisiae
    酿酒酵母的染色质富集组分的纯化方法—yChEFs
    作者:Abel Cuevas-Bermúdez, Ana I. Garrido-Godino, Francisco Gutiérrez-Santiago and Francisco Navarro日期:01/05/2020,浏览量:2219,Q&A: 0
    [Abstract] We have adapted a previous procedure and improved an approach that we named yChEFs (yeast Chromatin Enriched Fractions) for purifying chromatin fractions. This methodology allows the easy, reproducible and scalable recovery of proteins associated with chromatin. By using yChEFs, we bypass subcellular fractionation requirements involved when using ...
    Isolation of Outer Membrane Vesicles from Phytopathogenic Xanthomonas campestris pv. campestris
    从野油菜黄单胞菌野油菜致病变种分离外膜囊泡
    作者:Gideon Mordukhovich and Ofir Bahar日期:03/05/2017,浏览量:9092,Q&A: 5
    [Abstract] Gram-negative bacteria naturally release outer membrane vesicles (OMVs) to the surrounding environment. OMVs contribute to multiple processes, such as cell-cell communication, delivery of enzymes and toxins, resistance to environmental stresses and pathogenesis. Little is known about OMVs produced by plant-pathogenic bacteria, and their ...
    Preparation of Outer Membrane Vesicles from Myxococcus xanthus
    从黄色黏球菌制备外膜囊泡
    作者:James E. Berleman, Marcin Zemla, Jonathan P Remis and Manfred Auer日期:01/20/2016,浏览量:9233,Q&A: 0
    [Abstract] Outer membrane vesicles (OMVs) represent a unique sub-cellular compartment of bacteria that may act as a scaffold for various extracellular activities, including intercellular signaling. Myxococcus xanthus (M. xanthus) is a predatory bacterium that engages in cell-cell behaviors such as fruiting body formation and contact ...
    [14C] Linoleic Acid Uptake and Fractionation Assay in Vibrio cholerae
    霍乱弧菌中[14C] 亚油酸的摄取和分级分离试验
    作者:Sarah C. Plecha and Jeffrey H. Withey日期:12/20/2015,浏览量:5907,Q&A: 0
    [Abstract] The gram-negative curved bacillus Vibrio cholerae (V. cholerae) causes the severe diarrheal illness cholera. The work presented here is to assess whether unsaturated fatty acids (UFAs), such as linoleic acid, have the potential to directly affect proteins involved in DNA binding because they are able to enter the cell. In this ...
    Membrane Flotation Assay
    膜漂浮分析
    作者:Dorothee A Vogt and Melanie Ott日期:04/05/2015,浏览量:11661,Q&A: 0
    [Abstract] Many postitive-stranded RNA viruses, such as Hepatitis C virus (HCV), highjack cellular membranes, including the Golgi, ER, mitchondria, lipid droplets, and utilize them for replication of their RNA genome or assembly of new virions. By investigating how viral proteins associate with cellular membranes we will better understand the roles of ...
    Rapid Preparation of Unsheathed Bacterial Flagella
    未出鞘细菌鞭毛的快速制备
    作者:Soazig Le Guyon, Mikael Rhen and Ute Römling日期:03/20/2015,浏览量:8978,Q&A: 0
    [Abstract] The flagellum is required for bacterial swimming and swarming motility. In the biphasic Salmonella enterica serovar Typhimurium (S. Typhimurium), the flagellar filament is build up by two distinct monomeric subunits, flagellin FliC and FljB. S. Typhimurium has the ability to switch between two flagellins, FliC and FljB, ...
    Differentiation of Naturally Produced Extracellular Membrane Vesicles from Lipid Aggregation by Glucuronoxylomannan Immunogold Transmission Electron Microscopy in Bacillus subtilis
    采用Glucuronoxylomannan免疫胶体金透射电子显微镜法检测枯草杆菌中脂质聚合自然产生的胞外膜囊泡的分化
    作者:Lisa Brown, Geoff Perumal and Arturo Casadevall日期:03/05/2015,浏览量:8575,Q&A: 0
    [Abstract] Recently, membrane vesicle (MV) production was described in Gram-positive bacteria, which harbor a variety of components such as toxins, antibiotic resistance proteins, proteases, DNA, and immune modulators. Free lipids have the ability to form micelles, thus it is important to rule out spontaneous association of lipids into vesicle-like ...
    Vesicle Isolation from Bacillus subtilis Biofilm
    枯草杆菌生物膜的囊泡分离
    作者:Lisa Brown, Anne Kessler and Arturo Casadevall日期:03/05/2015,浏览量:10847,Q&A: 0
    [Abstract] Bacterial biofilms are associated clinically with many bacterial infections including those caused by bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus. In recent years, extracellular vesicles produced by bacteria have been isolated from biofilm communities. Vesicles have been described in depth and can ...
    Fractionation by Ultracentrifugation of Gram Negative Cytoplasmic and Membrane Proteins
    超速离心法分级分离革兰氏阴性菌细胞质和细胞膜蛋白
    作者:Sara M Sandrini, Richard Haigh and Primrose P. E. Freestone日期:11/05/2014,浏览量:17512,Q&A: 1
    [Abstract] Protein fractionation is a useful separation process which divides membrane proteins (including those located in the outer and inner membrane) and cytoplasmic proteins into discrete fractions. Fractionation of proteins can simplify analysis of the numbers of proteins present, and therefore make easier to characterize any environmentally or ...