细胞生物学

分类

    现刊
    High Throughput Traction Force Microscopy for Multicellular Islands on Combinatorial Microarrays
    组合微阵列多细胞岛的高通量牵引力显微镜实验
    作者:Ian C. Berg and Gregory H. Underhill日期:11/05/2019,浏览量:3709,Q&A: 0
    [Abstract] The composition and mechanical properties of the cellular microenvironment along with the resulting distribution of cellular devolved forces can affect cellular function and behavior. Traction Force Microscopy (TFM) provides a method to measure the forces applied to a surface by adherent cells. Numerous TFM systems have been described in ...
    An Image Analysis Pipeline to Quantify Emerging Cracks in Materials or Adhesion Defects in Living Tissues
    分析活体组织中出现的材料裂缝或附着力缺陷的图像分析法
    作者:Stéphane Verger, Guillaume Cerutti and Olivier Hamant日期:10/05/2018,浏览量:4271,Q&A: 0
    [Abstract] Microcracks in materials reflect their mechanical properties. The quantification of the number or orientation of such cracks is thus essential in many fields, including engineering and geology. In biology, cracks in soft tissues can reflect adhesion defects, and the analysis of their pattern can help to deduce the magnitude and orientation of ...
    Estimation of Stomatal Aperture in Arabidopsis thaliana Using Silicone Rubber Imprints
    使用硅橡胶印记估测拟南芥气孔开度
    作者:Telma E. Scarpeci, María I. Zanor and Estela M. Valle日期:06/20/2017,浏览量:9968,Q&A: 2
    [Abstract] Estimation of stomatal aperture using low viscosity silicone-base impression material has the advantage of working with the whole leaf. The developmental stage and the environment strongly affect the stomatal aperture. Therefore, it is mandatory to have accurate estimations of the stomatal aperture of intact leaves under different situations. With ...
    Measurement of Mechanical Tension at cell-cell junctions using two-photon laser ablation
    采用双光子激光烧蚀测定细胞间连接处的机械张力
    作者:Xuan Liang, Magdalene Michael and Guillermo A. Gomez日期:12/20/2016,浏览量:8125,Q&A: 0
    [Abstract] The cortical actomyosin cytoskeleton is found in all non-muscle cells where a key function is to control mechanical force (Salbreux et al., 2012). When coupled to E-cadherin cell-cell adhesion, cortical actomyosin generates junctional tension that influences many aspects of tissue function, organization and morphogenesis (Lecuit and Yap, ...
    Analysis of Myosin II Minifilament Orientation at Epithelial Zonula Adherens
    上皮粘着小带的微球蛋白II微丝排列分析
    作者:Magdalene Michael, Xuan Liang and Guillermo A. Gomez日期:12/05/2016,浏览量:7254,Q&A: 0
    [Abstract] Non-muscle myosin II (NMII) form bipolar filaments, which bind F-actin to exert cellular contractility during physiological processes (Vicente-Manzanares et al., 2009). Using a combinatorial approach to fluorescently label both N- and C-termini of the NMII heavy chain, recent works have demonstrated the ability to visualize NMII bipolar ...
    In vitro Microtubule Binding Assay and Dissociation Constant Estimation
    体外微管结合实验和解离常数估算
    作者:Christopher Kesten, René Schneider and Staffan Persson日期:03/20/2016,浏览量:12240,Q&A: 0
    [Abstract] Microtubules (MTs) support an astonishing set of versatile cellular functions ranging from cell division, vesicle transport, and cell and tissue morphogenesis in various organisms. This versatility is in large mediated by MT-associated proteins (MAPs). The neuronal MAP Tau, for example, is stabilizing MTs in axons of the vertebrate nervous system ...
    Imaging and Measurement of Nanomechanical Properties within Primary Xylem Cell Walls of Broadleaves
    阔叶初生木质部细胞壁内的纳米力学性能的成像和测定
    作者:Jaroslav Ďurkovič, Monika Kardošová and Rastislav Lagaňa日期:12/20/2014,浏览量:10837,Q&A: 0
    [Abstract] A technique of atomic force microscopy (AFM) called PeakForce quantitative nanomechanical mapping (PeakForce QNM) is an efficient tool for the quantitative mechanobiological imaging of fibrillar aggregate, human epidermal cell and woody plant cell wall topography (Sweers et al., 2011; Heu et al., 2012; Ďurkovič et al., ...
    Assay for Adherence of Vibrio cholerae to Eukaryotic Cell Lines
    霍乱弧菌在真核细胞系粘附性的分析
    作者:Amit K. Dey, Abha Bhagat and Rukhsana Chowdhury日期:04/20/2014,浏览量:9740,Q&A: 0
    [Abstract] Vibrio cholerae (V. cholerae) colonizes the intestinal epithelium and secretes cholera toxin (CT), a potent enterotoxin that causes severe fluid loss characteristic of the disease cholera. V. cholerae is a non-invasive Gram-negative bacterium that adheres to intestinal cells as well as a variety of different cell types. ...
    Adhesion of Moraxella catarrhalis to Respiratory Tract Epithelial Cells
    卡他莫拉菌在呼吸道上皮细胞的粘附试验
    作者:Stefan P.W. de Vries and Hester J. Bootsma日期:11/05/2013,浏览量:8198,Q&A: 0
    [Abstract] Moraxella catarrhalis is a human-restricted pathogen that is responsible for respiratory tract infections such as childhood otitis media (OM) and exacerbation of chronic obstructive pulmonary disease (COPD) in adults. Successful colonization and infection by M. catarrhalis depends on its ability to attach to the respiratory tract ...
    Analyzing Inhibitory Effects of Reagents on Mycoplasma Gliding and Adhesion
    试剂对支原体滑脱和粘附作用的抑制效应检测
    作者:Taishi Kasai and Makoto Miyata日期:07/20/2013,浏览量:10123,Q&A: 0
    [Abstract] Dozens of Mycoplasma species bind to solid surfaces and glide in the direction of the membrane protrusion at a pole. In gliding, Mycoplasma legs catch, pull and release sialylated oligosaccharides fixed on a solid surface. The analyses of inhibitory effects of sialylated compounds on gliding of Mycoplasma can determine ...