微生物学

分类

    现刊
    Bacterial Conjugation Protocol for Ruminant Mycoplasmas
    反刍动物支原体细菌接合的实验方法
    作者:Eveline Sagné, Christine Citti and Emilie Dordet-Frisoni日期:01/20/2021,浏览量:857,Q&A: 0
    [Abstract]

    In Mycoplasma agalactiae, two simultaneous processes of DNA transfer have been described that require direct cell-to-cell contact and are similar to conjugation. One involves the self-transmission of an integrative conjugative element (ICE) while the second concerns the horizontal transfer of large and small fragments of chromosomal DNA. Here, we

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    Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
    农杆菌介导的优良籼稻品种Komboka的高效转化
    [Abstract] Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of the world’s population. As a result, numerous transformation methods have been developed for both ...
    Isolation and Characterization of Live Yeast Cells from Ancient Clay Vessels
    从古代陶皿中分离鉴定活酵母细胞
    [Abstract] Ancient fermented food has been studied mainly based on residue analysis and recipes and reconstruction attempts were performed using modern domesticated yeast. Furthermore, microorganisms which participated in fermentation were studied using ancient-DNA techniques. In a recent paper, we presented a novel approach based on the hypothesis that ...
    Development of an Efficient Transformation System for Halotolerant Yeast Debaryomyces hansenii CBS767
    耐盐汉斯德巴氏酵母菌CBS767的一种高效转化体系的建立
    作者:Anu P. Minhas and Dipanwita Biswas日期:09/05/2019,浏览量:1929,Q&A: 0
    [Abstract] Debaryomyces hansenii is one of the most osmotolerant and halotolerant yeasts. Further, its association with traditional cheese and meat products imparting special flavors to these products project this yeast with enormous biotechnological potential in the agrofood sector. However, lack of an efficient transformation system in D. hansenii ...
    Measurement of the Length of the Integrated Donor DNA during Bacillus subtilis Natural Chromosomal Transformation
    枯草芽孢杆菌自然遗传转化过程中完整供体DNA长度的测定
    作者:Ester Serrano and Begoña Carrasco日期:08/20/2019,浏览量:1985,Q&A: 0
    [Abstract] For natural transformation to occur, bacterial cells must first develop a programmed physiological state called competence. Competence in Bacillus subtilis, which occurs only in a fraction of cells, is a transient stress response that allows cells to take up DNA from the environment. During natural chromosomal transformation, the ...
    Quantitative Transformation Efficiency Assay for Bacillus subtilis
    枯草芽孢杆菌转化效率的定量测定
    作者:Christian L. Loyo and Briana M. Burton日期:12/05/2018,浏览量:5257,Q&A: 0
    [Abstract] Bacillus subtilis (B. subtilis) is a model Gram-positive organism used to study a variety of physiological states and stress responses, one of which is the development of competence. Competence refers to the physiological state of a cell which allows it to be transformed naturally. Through induction of competence, the efficiency ...
    Induction of Natural Competence in Genetically-modified Lactococcus lactis
    转基因乳酸乳球菌自然感受态的诱导
    作者:Joyce Mulder, Michiel Wels, Oscar P. Kuipers, Michiel Kleerebezem and Peter A. Bron日期:07/05/2018,浏览量:4670,Q&A: 0
    [Abstract] Natural competence can be activated in Lactoccocus lactis subsp lactis and cremoris upon overexpression of ComX, a master regulator of bacterial competence. Herein, we demonstrate a method to activate bacterial competence by regulating the expression of the comX gene by using a nisin-inducible promoter in an L. ...
    Conjugation Assay for Testing CRISPR-Cas Anti-plasmid Immunity in Staphylococci
    接合实验检测葡萄球菌CRISPR-Cas的抗质粒免疫功能
    作者:Forrest C. Walker and Asma Hatoum-Aslan日期:05/05/2017,浏览量:9336,Q&A: 0
    [Abstract] CRISPR-Cas is a prokaryotic adaptive immune system that prevents uptake of mobile genetic elements such as bacteriophages and plasmids. Plasmid transfer between bacteria is of particular clinical concern due to increasing amounts of antibiotic resistant pathogens found in humans as a result of transfer of resistance plasmids within and between ...
    Polyethylene Glycol-mediated Transformation of Drechmeria coniospora
    聚乙二醇介导的圆锥掘氏梅里霉的转化
    作者:Le D. He and Jonathan J. Ewbank日期:03/05/2017,浏览量:7066,Q&A: 0
    [Abstract] Drechmeria coniospora is a nematophagous fungus and potential biocontrol agent. It belongs to the Ascomycota. It is related to Hirsutella minnesotensis, another nematophagous fungus but, phylogenetically, it is currently closest to the truffle parasite Tolypocladium ophioglossoides. Together with its natural host, ...
    Single-step Marker Switching in Schizosaccharomyces pombe Using a Lithium Acetate Transformation Protocol
    在粟酒裂殖酵母中采用醋酸锂转化方案进行标记基因的单步转换
    作者:Simon David Brown and Alexander Lorenz日期:12/20/2016,浏览量:6878,Q&A: 0
    [Abstract] The ability to utilize different selectable markers for tagging or mutating multiple genes in Schizosaccharomyces pombe is hampered by the historical use of only two selectable markers, ura4+ and kanMX6; the latter conferring resistance to the antibiotic G418 (geneticin). More markers have been described ...