微生物学

分类

    现刊
    Estimation of the Minimum Number of Replication Origins Per Chromosome in any Organism
    任何生物体内每个染色体的最小复制起源点数目的估计
    作者:Marcelo S. da Silva日期:10/20/2020,浏览量:1730,Q&A: 0
    [Abstract] Eukaryote nuclear genomes predominantly replicate through multiple replication origins. The number of replication origins activated per chromosome during the S-phase duration may vary according to many factors, but the predominant one is replication stress. Several studies have applied different approaches to estimate the number and map the ...
    Quantification of HIV-2 DNA in Whole Blood
    全血HIV-2 DNA的定量分析
    作者:Zsófia Szojka, Sara Karlson, Marianne Jansson and Patrik Medstrand日期:10/20/2019,浏览量:2947,Q&A: 0
    [Abstract] Time to AIDS infection is longer with HIV-2, compared to HIV-1, but without antiretroviral therapy both infections will cause AIDS-related mortality. In HIV-2 infection, monitoring of antiretroviral treatment (ART) efficacy is challenging since a large proportion of HIV-2-infected individuals displays low or undetectable plasma RNA levels. Hence, ...
    Strand-specific Single-stranded DNA Sequencing (4S-seq) of E. coli genomes
    大肠杆菌特异性单链DNA测序(4S-seq)
    作者:Takahiro Masuda, Nobuaki Kono, Masaru Tomita and Kazuharu Arakawa日期:08/05/2019,浏览量:4403,Q&A: 0
    [Abstract] Most bacterial genomes have biased nucleotide composition, and the asymmetry is considered to be caused by a single-stranded DNA (ssDNA) deamination arising from the bacterial replication machinery. In order to evaluate the relationship experimentally, the position and frequency of ssDNA formed during replication must be verified clearly. Although ...
    CRISPR/Cas9-mediated ssDNA Recombineering in Corynebacterium glutamicum
    谷氨酸棒状杆菌中CRISPR/Cas9介导的ssDNA重组工程技术
    作者:Jiao Liu, Yu Wang, Ping Zheng and Jibin Sun日期:10/05/2018,浏览量:4883,Q&A: 0
    [Abstract] Corynebacterium glutamicum is a versatile workhorse for industrial bioproduction of many kinds of chemicals and fuels, notably amino acids. Development of advanced genetic engineering tools is urgently demanded for systems metabolic engineering of C. glutamicum. Recently unveiled clustered regularly interspaced short palindromic ...
    ChIP-seq Experiment and Data Analysis in the Cyanobacterium Synechocystis sp. PCC 6803
    蓝藻集胞藻PCC 6803的ChIP-seq实验和数据分析
    作者:Joaquín Giner-Lamia, Miguel A. Hernández-Prieto and Matthias E. Futschik日期:06/20/2018,浏览量:9493,Q&A: 0
    [Abstract] Nitrogen is an essential nutrient for all living organisms. In cyanobacteria, a group of oxygenic photosynthetic bacteria, nitrogen homeostasis is maintained by an intricate regulatory network around the transcription factor NtcA. Although mechanisms controlling NtcA activity appear to be well understood, the sets of genes under its control (i.e. ...
    Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9
    使用CRISPR-Cas9对烈性噬菌体进行靶向基因组编辑
    作者:Marie-Laurence Lemay, Ariane C. Renaud, Geneviève M. Rousseau and Sylvain Moineau日期:01/05/2018,浏览量:12401,Q&A: 1
    [Abstract] This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial host, here the Gram-positive model Lactococcus lactis MG1363. The CRISPR-Cas9 system is ...
    Markerless Gene Editing in the Hyperthermophilic Archaeon Thermococcus kodakarensis
    超嗜热古细菌Thermococcus kodakarensis的无标记基因编辑
    作者:Alexandra M. Gehring, Travis J. Sanders and Thomas J. Santangelo日期:11/20/2017,浏览量:6010,Q&A: 0
    [Abstract] The advent of single cell genomics and the continued use of metagenomic profiling in diverse environments has exponentially increased the known diversity of life. The recovered and assembled genomes predict physiology, consortium interactions and gene function, but experimental validation of metabolisms and molecular pathways requires more ...
    Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences
    酿酒酵母中使用人工靶DNA序列进行多重CRISPR/ Cas9的方法
    作者:Rachael M. Giersch and Gregory C. Finnigan日期:09/20/2017,浏览量:9375,Q&A: 0
    [Abstract] Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that recruits the complex to a corresponding genomic target sequence where it induces a double stranded ...
    Detection of Pathogens and Ampicillin-resistance Genes Using Multiplex Padlock Probes
    使用多重锁式探针检测病原体和氨苄西林耐药基因
    作者:Rick Conzemius and Ivan Barišić日期:08/20/2017,浏览量:6767,Q&A: 0
    [Abstract] Diagnostic assays for pathogen identification and characterization are limited either by the number of simultaneously detectable targets, which rely on multiplexing methods, or by time constraints due to cultivation-based techniques. We recently presented a 100-plex method for human pathogen characterization to identify 75 bacterial and fungal ...
    Selection of Genetically Modified Bacteriophages Using the CRISPR-Cas System
    利用CRISPR-Cas系统筛选基因组编辑的噬菌体
    作者:Miriam Manor and Udi Qimron日期:08/05/2017,浏览量:8721,Q&A: 0
    [Abstract] We present a CRISPR-Cas based technique for deleting genes from the T7 bacteriophage genome. A DNA fragment encoding homologous arms to the target gene to be deleted is first cloned into a plasmid. The T7 phage is then propagated in Escherichia coli harboring this plasmid. During this propagation, some phage genomes undergo homologous ...