神经科学

分类

    现刊
    Dual Color, Live Imaging of Vesicular Transport in Axons of Cultured Sensory Neurons
    培养感觉神经元轴突囊泡运输的双色实时成像
    作者:Yoko Bekku and James L. Salzer日期:06/20/2021,浏览量:1068,Q&A: 0
    [Abstract]

    The function of neurons in afferent reception, integration, and generation of electrical activity relies on their strikingly polarized organization, characterized by distinct membrane domains. These domains have different compositions resulting from a combination of selective targeting and retention of membrane proteins. In neurons, most proteins

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    Generation of Mouse Primary Hypothalamic Neuronal Cultures for Circadian Bioluminescence Assays
    培养小鼠原发性下丘脑神经元用于昼夜生物发光分析
    作者:Cosima X. Schmidt, Anthony H. Tsang and Henrik Oster日期:03/05/2021,浏览量:971,Q&A: 0
    [Abstract]

    An endogenous circadian clock system enables organisms to adapt to time-of-day dependent environmental changes. In consequence, most physiological processes exhibit daily rhythms of, e.g., energy metabolism, immune function, sleep, or hormone production. Hypothalamic circadian clocks have been identified to play a particular role in coordinating

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    Establishing an Adult Mouse Brain Hippocampal Organotypic Slice Culture System that Allows for Tracing and Pharmacological Manipulation of ex vivo Neurogenesis
    用于追踪和离体神经发生药理学操作的成年小鼠脑器官型海马切片培养系统的建立
    作者:Steffen Mayerl and Charles ffrench-Constant日期:01/05/2021,浏览量:2064,Q&A: 0
    [Abstract]

    The function of the hippocampus depends on the process of adult hippocampal neurogenesis which underpins the exceptional neural plasticity of this structure, and is also frequently affected in CNS pathologies. Thus, manipulation of this process represents an important therapeutic goal. To identify potential strategies, organotypic adult brain

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    Co-culture of Murine Neurons Using a Microfluidic Device for The Study of Tau Misfolding Propagation
    利用微流体装置共培养小鼠神经元研究Tau的错误折叠传播
    作者:Grace I Hallinan, Dianne M Lopez, Mariana Vargas-Caballero, Jonathan West and Katrin Deinhardt日期:08/20/2020,浏览量:2059,Q&A: 0
    [Abstract] The deposition of misfolded, aggregated tau protein is a hallmark of several neurodegenerative diseases, collectively termed “tauopathies”. Tau pathology spreads throughout the brain along connected pathways in a prion-like manner. The process of tau pathology propagation across circuits is a focus of intense research and has been investigated in ...
    Generation of Functional Mouse Hippocampal Neurons
    功能性小鼠海马神经元的产生
    作者:Francesco Tomassoni-Ardori, Zhenyi Hong, Gianluca Fulgenzi and Lino Tessarollo日期:08/05/2020,浏览量:3097,Q&A: 1
    [Abstract] Primary culture of mouse hippocampal neurons is a very useful in vitro model for studying neuronal development, axonal and dendritic morphology, synaptic functions, and many other neuronal features. Here we describe a step-by-step process of generating primary neurons from mouse embryonic hippocampi (E17.5/E18.5). Hippocampal neurons ...
    A Simple and Efficient Method for Concomitant Isolation and Culture of Enriched Astroglial and Microglial Cells from the Rat Spinal Cord
    一种简单有效的大鼠脊髓星形胶质细胞和小胶质细胞联合分离培养方法
    作者:Pooja Shree Mishra and Trichur R Raju日期:01/20/2020,浏览量:2543,Q&A: 0
    [Abstract] Investigations into glial biology have contributed substantially in understanding the physiology and pathology of the nervous system. However, intricacies of the neuron-glial and glial-glial interactions in vivo present significant challenges while delineating the individual cell-type contributions, thus making the in vitro ...
    Myelin Oligodendrocyte Glycoprotein 35-55 (MOG 35-55)-induced Experimental Autoimmune Encephalomyelitis: A Model of Chronic Multiple Sclerosis
    髓鞘少突胶质细胞糖蛋白多肽( MOG35-55)诱发实验性自身免疫性脑脊髓炎:一种慢性多发性硬化模型
    作者:Sakie Miyamura, Nagisa Matsuo, Kazuki Nagayasu, Hisashi Shirakawa and Shuji Kaneko日期:12/20/2019,浏览量:3866,Q&A: 0
    [Abstract] Multiple sclerosis (MS) is the common demyelinating disease of human central nervous system. Among mouse models available to study MS, including the cuprizone application and lysolecithin-injection models, experimental autoimmune encephalomyelitis (EAE) model is widely used so that chronic EAE model of C57BL/6J can reflect the autoimmune ...
    Live-cell Migration Assays to Study Motility of Neural and Glial (Oligodendrocyte) Progenitor Cells
    利用活细胞运动分析研究神经和胶质(少突胶质细胞)祖细胞运动
    作者:Chu-Yen Chen, Fu-Sheng Chou and Pei-Shan Wang日期:06/20/2019,浏览量:3211,Q&A: 0
    [Abstract] Cell motility has been extensively studied in in vitro models using fibroblasts and keratocytes, but the cell type-specific mechanisms underlying migration of lineage- or disease-specific cells, such as neural and glial progenitor cells, remain an active field for investigation. The migrating neural and glial progenitor cells contribute ...
    Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons
    人诱导多能干细胞 (iPSCs) 分化为前脑神经祖细胞和成熟神经元的有效模型
    [Abstract] Induced Pluripotent Stem Cells (iPSCs) are pluripotent stem cells that can be generated from somatic cells, and provide a way to model the development of neural tissues in vitro. One particularly interesting application of iPSCs is the development of neurons analogous to those found in the human forebrain. Forebrain neurons play a central ...
    Collagenase-based Single Cell Isolation of Primary Murine Brain Endothelial Cells Using Flow Cytometry
    利用流式细胞技术进行原代小鼠脑内皮细胞的基于胶原酶的单细胞分离
    作者:Cathrin J. Czupalla, Hanadie Yousef, Tony Wyss-Coray and Eugene C. Butcher日期:11/20/2018,浏览量:7608,Q&A: 0
    [Abstract] The brain endothelium is a highly specialized vascular structure that maintains the activity and integrity of the central nervous system (CNS). Previous studies have reported that the integrity of the brain endothelium is compromised in a plethora of neuropathologies. Therefore, it is of particular interest to establish a method that enables ...