微生物学

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    现刊
    ODELAM: Rapid Sequence-independent Detection of Drug Resistance in Mycobacterium tuberculosis Isolates
    ODELAM:结核分枝杆菌耐药性的快速序列独立检测
    作者:Thurston Herricks, Magdalena Donczew, David R. Sherman and John D. Aitchison日期:05/20/2021,浏览量:1650,Q&A: 0
    [Abstract]

    Antimicrobial-resistant Mycobacterium tuberculosis (Mtb) causes over 200,000 deaths globally each year. Current assays of antimicrobial resistance require knowledge of the mutations that confer drug resistance or long periods of culture time to test growth under drug pressure. We present ODELAM (One-cell Doubling Evaluation of Living Arrays of

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    Screening for Lysogen Activity in Therapeutically Relevant Bacteriophages
    筛选与治疗相关的噬菌体的溶原活性
    作者:Fernando L. Gordillo Altamirano and Jeremy J. Barr日期:04/20/2021,浏览量:2686,Q&A: 1
    [Abstract]

    Lysogenic phages can integrate into their bacterial host’s genome, potentially transferring any genetic information they possess including virulence or resistance genes, and are therefore routinely excluded from therapeutic applications. Lysogenic behavior is typically seen in phages that create turbid plaques or possess subpar bactericidal

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    Determination of Antibacterial Activity of Film Coatings against Four Clinically Relevant Bacterial Strains
    薄膜敷层对4种与临床相关菌株的抑菌活性测定
    [Abstract]

    Antibacterial coatings have currently gained great importance in biomedical technology investigations. Because of the spatial arrangement of the film coatings, evaluation of antibacterial activity presents a new challenge regarding traditional bacterial counting methods. In this protocol, four clinically relevant pathogens, Salmonella typhimurium,

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    Antimicrobial Sensitivity Assay for Bdellovibrio bacteriovorus
    噬菌蛭弧菌的药敏试验
    作者:Emanuele Marine and Klaas M. Pos日期:12/20/2020,浏览量:1173,Q&A: 0
    [Abstract]

    Bdellovibrio bacteriovorus, an obligate predatory bacterium [i.e., bacteria that kill and feed on other bacteria (prey)], has the potential to be used as a probiotic for the disinfection of surfaces or for the treatment of bacterial infections. One option is to use this organism in combination with antimicrobials to potentiate the effectiveness of

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    Rapid Isolation and Purification of Secreted Bacteriocins from Streptococcus mutans and Other Lactic Acid Bacteria
    变形链球菌及其他乳酸菌分泌细菌素的快速分离纯化
    作者:Marcus Cheng, Siew-Ging Gong and Céline M. Lévesque日期:11/20/2020,浏览量:1905,Q&A: 0
    [Abstract] Bacteriocins are small ribosomally synthesized antimicrobial peptides produced by some microorganisms including lactic acid bacteria (LAB), a group of Gram-positive bacteria (cocci, rods) expressing high tolerance for low pH. Bacteriocins kill bacteria rapidly and are biologically active at very low concentrations. Bacteriocins produced by LAB ...
    Construction and Validation of A Low-cost, Small-scale, Multiplex Continuous Culturing System for Microorganisms
    低成本、小规模、复合微生物连续培养系统的构建与验证
    作者:Lilit Tonoyan, Freddy Guihéneuf, Ruari Friel and Vincent O’Flaherty日期:11/05/2020,浏览量:1202,Q&A: 0
    [Abstract]

    This protocol details the construction of a simple, low-cost, small-scale, multiplex chemostat system designed for the continuous cultivation of microorganisms in suspension (i.e., bacteria, yeast, microalgae). The continuous culture device can operate at a working volume of 25 ml and allows the run of 8 chemostats in parallel by a single person.

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    Contemporaneous Measurement of Outer and Inner Membrane Permeability in Gram-negative Bacteria
    革兰阴性菌中内外膜通透性的同期测定
    作者:Bo Ma, Chao Fang, Jing Zhang, Mingzhi Wang, Xiaoxing Luo and Zheng Hou日期:03/05/2020,浏览量:2324,Q&A: 0
    [Abstract] The emergence and rapid spread of multidrug resistance in bacteria have led to the urgent need for novel antibacterial agents. Membrane permeabilization is the mechanism for many antibacterial molecules that are being developed against gram-negative bacteria. Thus, to determine the efficacy of a potential antibacterial molecule, it is important to ...
    Rapid Detection of Proliferative Bacteria by Electrical Stimulation
    利用电刺激快速检测细菌增殖
    作者:Conor LA Edwards, Dmitry Malyshev, James P Stratford and Munehiro Asally日期:02/05/2020,浏览量:3124,Q&A: 0
    [Abstract] Detecting live bacteria is an important task for antimicrobial susceptibility testing (AST) in the medical sector and for quality-monitoring in biological industries. Current methods for live-bacteria detection suffer limitations in speed or sensitivity. In a recent paper, we reported that electrical response dynamics in membrane potential enable ...
    Assessing Membrane Fluidity and Visualizing Fluid Membrane Domains in Bacteria Using Fluorescent Membrane Dyes
    利用荧光膜染料评估膜流动性以及观察细菌中的流体膜结构区域
    作者:Michaela Wenzel, Norbert O. E. Vischer, Henrik Strahl and Leendert W. Hamoen日期:10/20/2018,浏览量:7015,Q&A: 0
    [Abstract] Membrane fluidity is a key parameter of bacterial membranes that undergoes quick adaptation in response to environmental challenges and has recently emerged as an important factor in the antibacterial mechanism of membrane-targeting antibiotics. The specific level of membrane fluidity is not uniform across the bacterial cell membrane. Rather, ...
    Bacterial Aggregation Assay in the Presence of Cyclic Lipopeptides
    环脂肽存在情况下细菌聚集实验
    作者:Pengyuan Xiu, Rui Liu, Dechao Zhang and Chaomin Sun日期:01/05/2018,浏览量:6136,Q&A: 0
    [Abstract] Lipopeptides is an important class of biosurfactants having antimicrobial and anti-adhesive activity against pathogenic bacteria. These include surfactin, fengycin, iturin, bacillomycin, mycosubtilin, lichenysin, and pumilacidin (Arima et al., 1968; Naruse et al., 1990; Yakimov et al., 1995; Steller and Vater, 2000; ...