植物科学

分类

    现刊
    Histochemical Staining of Suberin in Plant Roots
    植物根部软木脂的组织化学染色
    作者:Peter Marhavý and Shahid Siddique日期:02/05/2021,浏览量:2163,Q&A: 0
    [Abstract]

    Histological stains are useful tools for characterizing cell shape, arrangement and the material they are made from. Stains can be used individually or simultaneously to mark different cell structures or polymers within the same cells, and to visualize them in different colors. Histological stains can be combined with genetically-encoded

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    Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa
    大麻种子快速高效发芽规程的建立与标准化
    作者:Aleksei Sorokin, Narendra Singh Yadav, Daniel Gaudet and Igor Kovalchuk日期:01/05/2021,浏览量:1806,Q&A: 0
    [Abstract]

    Cannabis seed germination is an important process for growers and researchers alike. Many biotechnological applications require a reliable sterile method for seed germination. This protocol outlines a seed germination procedure for Cannabis sativa using a hydrogen peroxide (H2O2) solution as liquid germination media. In this protocol, all three

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    Transient Expression Assay in Strawberry Fruits
    草莓果实中的瞬时表达检测
    作者:Mengting Pi, Qi Gao and Chunying Kang日期:06/05/2019,浏览量:4733,Q&A: 0
    [Abstract] Strawberry, including the woodland strawberry Fragaria vesca (2x) and the cultivated strawberry (Fragaria × ananassa, 8x), has emerged as a model system for studying fruit development and ripening. Transient expression provides a quick assay for gene functions or gene interactions. In strawberry, ...
    Micro-computed Tomography to Visualize Vascular Networks in Maize Stems
    玉米茎杆中维管网络的微型计算机断层摄影术观察
    作者:Akiteru Maeno and Katsutoshi Tsuda日期:01/05/2018,浏览量:7443,Q&A: 0
    [Abstract] Plant vascular systems in the stem connect roots with aerial organs to move solutes containing minerals, nutrients as well as signaling molecules, and therefore, they play pivotal roles in plant growth and development. However, stem vascular systems, especially in crop species, have been poorly described since they are deeply embedded in the ...
    Investigating the Shape of the Shoot Apical Meristem in Bamboo Using a Superellipse Equation
    采用超椭圆方程研究竹顶端分生组织的形态
    作者:Qiang Wei and Peijian Shi日期:12/05/2017,浏览量:4481,Q&A: 0
    [Abstract] The shoot apical meristem is the origin of bamboo wood. Its structure and morphology are important for maintaining the normal development of bamboo wood. However, the traditional method to describe the morphology of the shoot apical meristem in bamboo or other plants only depends on qualitative approaches. Here we present a protocol for precisely ...
    Non-invasive Protocol for Kinematic Monitoring of Root Growth under Infrared Light
    红外线下根生长运动学的无创监测实验方案
    [Abstract] Phenotyping the dynamics of root responses to environmental cues is necessary to understand plant acclimation to their environment. Continuous monitoring of root growth is challenging because roots normally grow belowground and are very sensitive to their growth environment. This protocol combines infrared imaging with hydroponic cultivation for ...
    Multiplexed GuideRNA-expression to Efficiently Mutagenize Multiple Loci in Arabidopsis by CRISPR-Cas9
    多重向导RNA表达通过CRISPR Cas9对拟南芥中的多个基因座进行高效诱变
    作者:Julia Schumacher, Kerstin Kaufmann and Wenhao Yan日期:03/05/2017,浏览量:9516,Q&A: 0
    [Abstract] Since the discovery of the CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein (Cas) as an efficient tool for genome editing in plants (Li et al., 2013; Shan et al., 2013; Nekrasov et al., 2013), a large variety of applications, such as gene knock-out, knock-in or transcriptional ...
    Electro-fusion of Gametes and Subsequent Culture of Zygotes in Rice
    水稻配子的电融合及后续合子培养
    作者:Erika Toda, Yukinosuke Ohnishi and Takashi Okamoto日期:12/20/2016,浏览量:8791,Q&A: 0
    [Abstract] Electro-fusion system with isolated gametes has been utilized to dissect fertilization-induced events in angiosperms, such as egg activation, zygote development and early embryogenesis, since the female gametophytes of plants are deeply embedded within ovaries. In this protocol, procedures for isolation of rice gametes, electro-fusion of gametes, ...
    Strategies for Performing Dynamic Gene Perturbation Experiments in Flowers
    在花朵中进行动态基因扰动的实验策略
    作者:Diarmuid S. Ó’Maoiléidigh, Emmanuelle Graciet and Frank Wellmer日期:04/05/2016,浏览量:6995,Q&A: 0
    [Abstract] Dissecting the gene regulatory networks (GRNs) underlying developmental processes is a central goal in biology. The characterization of the GRNs underlying flower development has received considerable attention, however, novel approaches are required to reveal temporal and spatial aspects of these GRNs. Here, we provide an overview of the options ...
    In vitro Microtubule Binding Assay and Dissociation Constant Estimation
    体外微管结合实验和解离常数估算
    作者:Christopher Kesten, René Schneider and Staffan Persson日期:03/20/2016,浏览量:12138,Q&A: 0
    [Abstract] Microtubules (MTs) support an astonishing set of versatile cellular functions ranging from cell division, vesicle transport, and cell and tissue morphogenesis in various organisms. This versatility is in large mediated by MT-associated proteins (MAPs). The neuronal MAP Tau, for example, is stabilizing MTs in axons of the vertebrate nervous system ...