微生物学

分类

    现刊
    Primer ID Next-Generation Sequencing for the Analysis of a Broad Spectrum Antiviral Induced Transition Mutations and Errors Rates in a Coronavirus Genome
    用于分析冠状病毒基因组中广谱抗病毒药物诱导的过渡突变和错误率的引物ID下一代测序
    作者:Shuntai Zhou, Collin S. Hill, Michael U. Clark, Timothy P. Sheahan, Ralph Baric and Ronald Swanstrom日期:03/05/2021,浏览量:3066,Q&A: 0
    [Abstract]

    Next generations sequencing (NGS) has become an important tool in biomedical research. The Primer ID approach combined with the MiSeq platform overcomes the limitation of PCR errors and reveals the true sampling depth of population sequencing, making it an ideal tool to study mutagenic effects of potential broad-spectrum antivirals on RNA viruses.

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    Analysis of the Effect of Sphingomyelinase on Rubella Virus Infectivity in Two Cell Lines
    在两细胞系中分析鞘磷脂酶对于风疹病毒感染力的影响
    作者:Noriyuki Otsuki, Masafumi Sakata, Yoshio Mori, Kiyoko Okamoto and Makoto Takeda日期:09/05/2018,浏览量:3523,Q&A: 0
    [Abstract] Rubella is a mildly contagious disease characterized by low-grade fever and a morbilliform rash caused by the rubella virus (RuV). Viruses often use cellular phospholipids for infection. We studied the roles of cellular sphingomyelin in RuV infection. Treatment of cells with sphingomyelinase (SMase) inhibited RuV infection in rabbit kidney-derived ...
    Virucidal and Neutralizing Activity Tests for Antiviral Substances and Antibodies
    抗病毒物质和抗体的杀灭病毒活性及中和活性检测
    作者:Chie Aoki-Utsubo, Ming Chen and Hak Hotta日期:05/20/2018,浏览量:9145,Q&A: 0
    [Abstract] In a narrow definition, virucidal activity represents the activity by which to interact with and physically disrupt viral particles. In a broad definition, it includes the activity by which to functionally inhibit (neutralize) viral infectivity without apparent morphological alterations of the viral particles. The viral infectivity can be measured ...
    Time-of-addition and Temperature-shift Assays to Determine Particular Step(s) in the Viral Life Cycle that is Blocked by Antiviral Substance(s)
    改变药物添加时间及变温分析实验确定抗病毒物质所作用的病毒生命周期中的步骤
    作者:Chie Aoki-Utsubo, Ming Chen and Hak Hotta日期:05/05/2018,浏览量:6101,Q&A: 0
    [Abstract] Viruses infect their host cells to produce progeny virus particles through the sequential steps of the viral life cycle, such as viral attachment, entry, penetration and post-entry events. This protocol describes time-of-addition and temperature-shift assays that are employed to explore which step(s) in the viral life cycle is blocked by an ...
    Nab Escaping AAV Mutants Isolated from Mouse Muscles
    从小鼠肌肉中分离Nab逃脱AAV突变体
    作者:Zheng Chai, R. Jude Samulski and Chengwen Li日期:05/05/2018,浏览量:4765,Q&A: 0
    [Abstract] Neutralizing antibodies (Nabs) are a major challenge in clinical trials of adeno-associated virus (AAV) vector gene therapy, because Nabs are able to inhibit AAV transduction in patients. We have successfully isolated several novel Nab-escaped AAV chimeric capsids in mice by administrating a mixture of AAV shuffled library and patient serum. These ...
    In vitro Assay to Assess Efficacy of Potential Antiviral Compounds against Enterovirus D68
    体外实验评估候选的抗病毒化合物对肠道病毒D68的疗效
    作者:Liang Sun, Leen Delang, Carmen Mirabelli and Johan Neyts日期:03/20/2017,浏览量:7681,Q&A: 0
    [Abstract] In 2014 enterovirus D68 (EV-D68) caused the largest outbreak in the United States since the discovery of the virus. Distinct from before, the 2014 infections were associated with more severe respiratory disease and occasional neurological complications. So far, there are no available vaccines or antivirals for the prophylaxis or treatment of ...
    In vitro Studies: Inhibition of Nevirapine Metabolism by Nortriptyline in Hepatic Microsomes
    体外研究:肝脏微粒体中去甲替林对奈韦拉平新陈代谢的抑制
    作者:Iris Usach and José-Esteban Peris日期:10/05/2015,浏览量:6527,Q&A: 0
    [Abstract] One of the most prevalent and interfering psychosocial comorbidities of HIV infection is clinical depression (22 to 45%). For this reason, a study of a possible interaction between the nonnucleoside reverse transcriptase inhibitor nevirapine (NVP) and the tricyclic antidepressant nortriptyline (NT) was carried out. In vitro studies with ...
    RNase H Polymerase-independent Cleavage Assay for Evaluation of RNase H Activity of Reverse Transcriptase Enzymes
    RNase H 聚合酶依赖性分裂试验评估逆转录酶中Rnase H的活性
    作者:Angela Corona and Enzo Tramontano日期:08/20/2015,浏览量:10558,Q&A: 0
    [Abstract] The ribonuclease H (RNase H) polymerase-independent cleavage assay allows detection and quantification of RNase H activity of reverse transcriptase (RT) enzymes with a hybrid substrate formed by a fluorescein labeled RNA annealed with Dabcyl DNA (Figure 1). Here we describe a protocol that we have adapted for HIV-1 RT expressed from a ...
    In vitro Drug Susceptibility Assay for HBV Using Southern Blotting
    采用Southern蛋白印迹法进行HBV体外药敏性试验
    作者:Sung Hyun Ahn, Yong Kwang Park and Kyun-Hwan Kim日期:04/20/2015,浏览量:9009,Q&A: 0
    [Abstract] Antiviral agents for the suppression of hepatitis B virus (HBV) have been used for treating chronic hepatitis B. However, the emergence of drug-resistant HBV is still a major problem for antiviral treatment. To identify and characterize the drug-resistant HBV, the construction of HBV replicon and in vitro drug susceptibility assay are ...
    Introduction and Sequencing of Patient-isolated HBV RT Sequences into the HBV 1.2-mer Replicon
    把亲体分离的HBV RT序列引入HBV 1.2-mer复制子中并测序
    作者:Sung Hyun Ahn, Yong Kwang Park and Kyun-Hwan Kim日期:04/20/2015,浏览量:7456,Q&A: 0
    [Abstract] Antiviral agents for the suppression of hepatitis B virus (HBV) have been used for treating chronic hepatitis B. However, the emergence of drug-resistant HBV is still a major problem for antiviral treatment. To identify and characterize the drug-resistant HBV, the construction of HBV replicon and in vitro drug susceptibility assay are ...