免疫学

分类

    现刊
    Super-resolution Imaging of the T cell Central Supramolecular Signaling Cluster Using Stimulated Emission Depletion Microscopy
    利用受激发射损耗显微镜对T细胞中央超分子信号簇的超分辨成像
    作者:Sin Lih Tan, Dominic Alibhai, Stephen J. Cross, Harry Thompson and Christoph Wülfing日期:11/05/2020,浏览量:1232,Q&A: 0
    [Abstract]

    Supramolecular signaling assemblies are of interest for their unique signaling properties. A µm scale signaling assembly, the central supramolecular signaling cluster (cSMAC), forms at the center interface of T cells activated by antigen presenting cells (APC). The adaptor protein linker for activation of T cells (LAT) is a key cSMAC component.

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    Site-specific Labeling of B Cell Receptor and Soluble Immunoglobulin
    B细胞受体和可溶性免疫球蛋白的定位标记
    作者:Yu Wang, Zhixun Shen, Zhengpeng Wan and Wanli Liu日期:09/20/2020,浏览量:2488,Q&A: 0
    [Abstract] B lymphocyte activation is regulated by its membrane-bound B cell receptors (BCRs) upon recognizing diverse antigens. It is hypothesized that antigen binding would trigger conformational changes within BCRs, followed by a series of downstream signaling activation. To measure the BCR conformational changes in live cells, a fluorescent site-specific ...
    Characterization of Mouse Adult Testicular Macrophage Populations by Immunofluorescence Imaging and Flow Cytometry
    免疫荧光成像和流式细胞术鉴定小鼠成人睾丸巨噬细胞群体
    作者:Noushin Mossadegh-Keller and Michael H. Sieweke日期:03/05/2019,浏览量:3911,Q&A: 0
    [Abstract] Testicular macrophages (tMΦ) are the most abundant immune cells residing in the testis, an immune-privileged organ. TMΦ are known to exhibit different functions, such as protecting spermatozoa from auto-immune attack by producing immunosuppressive cytokines and trophic roles in supporting spermatogenesis and male sex hormone production. They also ...
    Immunohistochemical Staining of TLR4 in Human Skeletal Muscle Samples
    人类骨骼肌样本中TLR4的免疫组化染色
    作者:Daniela Verzola, Samantha Milanesi, Francesca Costigliolo and Giacomo Garibotto日期:01/20/2019,浏览量:3735,Q&A: 0
    [Abstract] Growing evidence suggests the involvement of TLR4, a receptor in the innate immune system, in muscle loss in uremia. Recently, we have evaluated TLR4 in human skeletal muscle from chronic kidney disease patients, by immunohistochemistry and image analysis. Unlike the commonly-used Western blot method, immunohistochemistry allows for the ...
    Quantification of Extracellular Double-stranded RNA Uptake and Subcellular Localization Using Flow Cytometry and Confocal Microscopy
    流式细胞术和共聚焦显微镜检查定量测定细胞外双链RNA摄取和亚细胞定位
    作者:Tan A Nguyen, Lachlan Whitehead and Ken C Pang日期:06/20/2018,浏览量:4758,Q&A: 0
    [Abstract] Double-stranded RNA is a potent pathogen-associated molecular pattern (PAMP) produced as a by-product of viral replication and a well-known hallmark of viral infection. Viral dsRNAs can be released from infected cells into the extracellular space and internalized by neighboring cells via endocytosis. Mammals possess multiple pattern recognition ...
    Proximal Ligation Assay (PLA) on Lung Tissue and Cultured Macrophages to Demonstrate Protein-protein Interaction
    对肺组织和培养的巨噬细胞进行邻位连接分析 (PLA) 以验证蛋白质 - 蛋白质相互作用
    作者:Roberto Mendez and Santanu Banerjee日期:11/05/2017,浏览量:8109,Q&A: 0
    [Abstract] In this protocol, we describe proximal ligation assay (PLA), an antibody-based detection method for protein-protein interaction. This method relies on specific binding of individual primary antibodies to the two putative interacting proteins. The primary antibodies need to have different hosts. The secondary antibodies against the two hosts have ...
    In vitro Detection of Neutrophil Traps and Post-attack Cell Wall Changes in Candida Hyphae
    念珠菌菌丝中嗜中性粒细胞诱捕和攻击后细胞壁变化的体外检测
    作者:Alex Hopke and Robert T. Wheeler日期:04/05/2017,浏览量:7240,Q&A: 0
    [Abstract] In this protocol we describe how to visualize neutrophil extracellular traps (NETs) and fungal cell wall changes in the context of the coculture of mouse neutrophils with fungal hyphae of Candida albicans. These protocols are easily adjusted to test a wide array of hypotheses related to the impact of immune cells on fungi and the cell ...
    Ear Inflammation and Whole-mount Ear Staining
    耳部炎症和耳部整体染色
    作者:Jia Tong Loh, Merry Gunawan and I-hsin Su日期:10/20/2016,浏览量:8673,Q&A: 0
    [Abstract] The recruitment of circulating neutrophils from the bloodstream to the site of inflammation represents one of the earliest events during an innate immune response. During this response, neutrophils tether and roll along the vessel walls before transmigrating across the endothelium into the interstitial space to exert their functions. Here, we ...
    Imaging Thick Lymph Node Tissue Sections
    淋巴结组织厚切片的成像技术
    作者:Jyh Liang Hor and Scott N. Mueller日期:09/20/2016,浏览量:10504,Q&A: 0
    [Abstract] Our protocol describes a simple procedure for imaging thick lymph node sections by 2-photon microscopy. Lymph nodes are sectioned using a vibratome (vibrating microtome) to produce slices of tissue that can then be stained with fluorescently labeled antibodies. The thick tissue sections (150-200 μm depth) allow for the detection of cell clustering ...
    Confocal Imaging of Myeloid Cells in the Corneal Stroma of Live Mice
    小鼠角膜基质中骨髓细胞的活体共聚焦成像
    作者:Matilda F. Chan and Zena Werb日期:07/05/2015,浏览量:10040,Q&A: 0
    [Abstract] The accessibility and transparency of the cornea makes it a good tissue model for monitoring immunological responses using in vivo real time imaging analysis (Lee et al., 2010; Tan et al., 2013). These corneal qualities have also allowed for high-resolution in vivo imaging of non-ocular tissue transplanted into ...