发育生物学

分类

    现刊
    Isolation of Stem Cells, Endothelial Cells and Pericytes from Human Infantile Hemangioma
    人婴幼儿血管瘤中干细胞、内皮细胞和周细胞的分离
    作者:Lan Huang and Joyce Bischoff日期:01/20/2020,浏览量:2033,Q&A: 0
    [Abstract] Infantile hemangioma (IH) is a vascular tumor noted for its excessive blood vessel formation during infancy, glucose-transporter-1 (GLUT1)-positive staining of the blood vessels, and its slow spontaneous involution over several years in early childhood. For most children, IH poses no serious threat because it will eventually involute, but a subset ...
    Human Endothelial Cell Spheroid-based Sprouting Angiogenesis Assay in Collagen
    在胶原蛋白中分析人内皮细胞基于球体的出芽式血管生成
    作者:Fabian Tetzlaff and Andreas Fischer日期:09/05/2018,浏览量:11977,Q&A: 0
    [Abstract] Angiogenesis, the formation of new blood vessels from pre-existing ones plays an important role during organ development, regeneration and tumor progression. The spheroid-based sprouting assay is a well-established and robust method to study the influence of genetic alterations or pharmacological compounds on capillary-like tube formation of ...
    In vitro Co-culture of Mesenchymal Stem Cells and Endothelial Colony Forming Cells
    间充质干细胞和内皮集落形成细胞的体外共培养
    作者:Abbas Shafiee and Kiarash Khosrotehrani日期:10/20/2017,浏览量:11230,Q&A: 0
    [Abstract] The discovery of endothelial colony forming cells (ECFCs) with robust self-renewal and de novo vessel formation potentials suggests that ECFCs can be an excellent cell source for cardiovascular diseases treatment through improving neovascularization in the ischemic tissues. However, their engraftment after transplantation resulted to be low. ...
    Vascular Smooth Muscle Cell Isolation and Culture from Mouse Aorta
    小鼠主动脉血管平滑肌细胞的分离和培养
    作者:Callie S. Kwartler, Ping Zhou, Shao-Qing Kuang, Xue-Yan Duan, Limin Gong and Dianna M. Milewicz日期:12/05/2016,浏览量:17392,Q&A: 0
    [Abstract] Vascular smooth muscle cells (SMC) in the ascending thoracic aorta arise from neural crest cells, whereas SMCs in the descending aorta are derived from the presomitic mesoderm. SMCs play important roles in cardiovascular development and aortic aneurysm formation. This protocol describes the detailed process for explanting ascending and descending ...
    Aorta Ring Assay
    主动脉环试验
    作者:Jing Jin, Xinyang Hu, Ling Zhang and Jian'an Wang日期:07/05/2016,浏览量:11614,Q&A: 2
    [Abstract] Angiogenesis is the nature and pathological process of blood vessel growth from pre-existing vascular buds. It plays an important role in cancer and cardiovascular disease. The aorta ring assay is an approach to study angiogenesis. In this experiment, we used the aorta of rat as the study material, cleaned the surrounding tissue of aorta and cut ...
    Mouse Retinal Whole Mounts and Quantification of Vasculature Protocol
    小鼠视网膜整装和脉管系统量化的实验方案
    作者:Irit Adini and Kaustabh Ghosh日期:08/05/2015,浏览量:14056,Q&A: 1
    [Abstract] Angiogenesis is the formation of new blood vessels from a pre-existing vascular bed. It is a multi-step process beginning with enzymatic degradation of the capillary basement membrane, followed by endothelial cell (EC) proliferation, migration, tube formation, assembly of a new basement membrane, and pericyte stabilization. Aberrant angiogenesis ...