微生物学

分类

    现刊
    Characterize the Interaction of the DNA Helicase PriA with the Stalled DNA Replication Fork Using Atomic Force Microscopy
    用原子力显微镜描述DNA解旋酶PriA与停止的DNA复制叉的相互作用
    作者:Yaqing Wang, Zhiqiang Sun, Piero R. Bianco and Yuri L. Lyubchenko日期:03/05/2021,浏览量:1348,Q&A: 0
    [Abstract]

    In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. ssDNA-binding protein (SSB) is typically present at the abandoned forks,

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    Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids
    受荧光标记和易于固化质粒影响的假单胞菌快速基因组工程
    作者:Daniel C. Volke, Nicolas T. Wirth and Pablo I. Nikel日期:02/20/2021,浏览量:2213,Q&A: 0
    [Abstract]

    Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted methods, homologous recombination, or λ

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    Combining Gel Retardation and Footprinting to Determine Protein-DNA Interactions of Specific and/or Less Stable Complexes
    结合凝胶阻滞和足印法测定特异性和/或不稳定复合物的蛋白质-DNA相互作用
    作者:Meng-Lun Hsieh, Alice Boulanger, Leslie G. Knipling and Deborah M. Hinton日期:12/05/2020,浏览量:853,Q&A: 0
    [Abstract]

    DNA footprinting is a classic technique to investigate protein-DNA interactions. However, traditional footprinting protocols can be unsuccessful or difficult to interpret if the binding of the protein to the DNA is weak, the protein has a fast off-rate, or if several different protein-DNA complexes are formed. Our protocol differs from traditional

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    Site-specific DNA Mapping of Protein Binding Orientation Using Azidophenacyl Bromide (APB)
    叠氮苯酰溴(APB)定位蛋白质结合方向的DNA图谱
    作者:Himasha M. Perera and Michael A. Trakselis日期:06/20/2020,浏览量:1848,Q&A: 0
    [Abstract] The orientation of a DNA-binding protein bound on DNA is determinative in directing the assembly of other associated proteins in the complex for enzymatic action. As an example, in a replisome, the orientation of the DNA helicase at the replication fork directs the assembly of the other associated replisome proteins. We have recently determined ...
    Quantification of Hepatitis B Virus Covalently Closed Circular DNA in Infected Cell Culture Models by Quantitative PCR
    qPCR定量分析感染细胞培养模型中乙型肝炎病毒共价闭合环状DNA
    作者:Bingqian Qu and Stephan Urban日期:04/05/2019,浏览量:4269,Q&A: 0
    [Abstract] Persistence of the human hepatitis B virus (HBV) requires the maintenance of covalently closed circular (ccc)DNA, the episomal genome reservoir in nuclei of infected hepatocytes. cccDNA elimination is a major aim in future curative therapies currently under development. In cell culture based in vitro studies, both hybridization- and ...
    Analysis of DNA Exchange Using Thymidine Analogs (ADExTA) in Trypanosoma cruzi
    克氏锥虫中利用胸苷类似物进行DNA交换分析
    作者:Marcelo S. da Silva, Paula A. Marin, Bruno M. Repolês, Maria C. Elias and Carlos R. Machado日期:12/20/2018,浏览量:2662,Q&A: 0
    [Abstract] Trypanosoma cruzi is a protozoan parasite belonging to the Trypanosomatidae family. Although the trypanosomatids multiply predominantly by clonal generation, the presence of DNA exchange in some of them has been puzzling researchers over the years, mainly because it may represent a novel form that these organisms use to gain variability. ...
    Advanced Design of Minimalistic Dumbbell-shaped Gene Expression Vectors
    极简化哑铃形基因表达载体的优化设计
    作者:Xiaoou Jiang and Volker Patzel日期:08/05/2017,浏览量:7051,Q&A: 0
    [Abstract] Minimal DNA vectors exclusively comprising therapeutically relevant sequences hold great promise for the development of novel therapeutic regimen. Dumbbell-shaped vectors represent non-viral non-integrating DNA minimal vectors which have entered an advanced stage of clinical development (Hardee et al., 2017). Spliceable introns and DNA ...
    Conjugation Assay for Testing CRISPR-Cas Anti-plasmid Immunity in Staphylococci
    接合实验检测葡萄球菌CRISPR-Cas的抗质粒免疫功能
    作者:Forrest C. Walker and Asma Hatoum-Aslan日期:05/05/2017,浏览量:9089,Q&A: 0
    [Abstract] CRISPR-Cas is a prokaryotic adaptive immune system that prevents uptake of mobile genetic elements such as bacteriophages and plasmids. Plasmid transfer between bacteria is of particular clinical concern due to increasing amounts of antibiotic resistant pathogens found in humans as a result of transfer of resistance plasmids within and between ...
    MNase Digestion for Nucleosome Mapping in Neurospora
    微球菌核酸酶消化法分析脉孢菌中核小体图谱
    作者:Cigdem Sancar, Gencer Sancar and Michael Brunner日期:06/05/2016,浏览量:7671,Q&A: 0
    [Abstract] Digestion of chromatin by micrococcal nuclease MNase followed by high throughput sequencing allows us to determine the location and occupancy of nucleosomes on the genome. Here in this protocol we have described optimized conditions of MNase digestion of filamentous fungus Neurospora crassa chromatin without a requirement of a nuclear ...
    Characterization of HBV Isolates from Patient Serum Samples and Cloning
    病人血清样本和克隆中HBV分离体的特性描述
    作者:Liang Cao, Chunchen Wu, Kaitao Zhao and Xinwen Chen日期:12/20/2015,浏览量:7564,Q&A: 0
    [Abstract] Hepatitis B virus (HBV) mutants can lead to vaccine failure, diagnostic failure of HBV detection, increase viral replication and resistance to antiviral agents. To study the biological characteristics of these mutations may contribute to our knowledge on viral pathogenesis. Therefore, it is essential to isolate and characterize HBV strains from ...