神经科学

分类

    现刊
    Ex vivo Tissue Culture Protocols for Studying the Developing Neocortex
    研究发育中的新皮质的体外组织培养方案
    作者:Takashi Namba, Christiane Haffner and Wieland B. Huttner日期:05/20/2021,浏览量:1897,Q&A: 0
    [Abstract]

    The size of the neocortex and its morphology are highly divergent across mammalian species. Several approaches have been utilized for the analysis of neocortical development and comparison among different species. In the present protocol (Note: This protocol requires basic knowledge of brain anatomy), we describe three ex vivo neocortical

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    Organotypic Slice Culture of the Embryonic Mouse Brain
    胚胎小鼠大脑的器官型切片培养
    作者:James M. Clegg and Thomas Pratt日期:07/05/2020,浏览量:1749,Q&A: 0
    [Abstract] Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.
    Explant Culture of the Embryonic Mouse Spinal Cord and Gene Transfer by ex vivo Electroporation
    小鼠胚胎脊髓的组织块培养和体外电穿孔介导的基因转移
    [Abstract] Developing axons change responsiveness to guidance cues during the journey to synapse with target cells. Axon crossing at the ventral midline serves as a model for studying how axons accomplish such a switch in their response. Although primary neuron culture has been a versatile technique for elucidating various developmental mechanisms, many in ...
    Organotypic Explants of the Embryonic Rodent Hippocampus: An Accessible System for Transgenesis
    啮齿动物胚胎海马器官型外植体:一个可进行转基因的系统
    作者:Archana Iyer , Lakshmi Subramanian and Shubha Tole日期:03/20/2018,浏览量:5195,Q&A: 0
    [Abstract] This protocol describes the technique of ex-vivo electroporation to target embryonic hippocampal progenitors in an organotypic slice preparation. This technique allows gene perturbation for examining developmental processes in the embryonic hippocampus while retaining the environment and connectivity of the cells. Gene perturbation can ...
    Mandibular Explant Assay for Investigating Extrinsic Stimuli on Bone and Cartilage Development
    研究骨及软骨外源刺激的下颌外植体实验
    作者:Jamie Jiang, Jessica W. Bertol and Walid D. Fakhouri日期:12/05/2017,浏览量:5635,Q&A: 0
    [Abstract] A major issue in developmental biology is to determine how embryonic tissues respond to molecular signals in a timely manner and given the position-restricted instructions during morphogenesis, of which Meckel’s cartilage is a classical example. The ex-vivo explant model is a practical and convenient system that allows investigation of ...
    Dissection and Staining of Mouse Brain Ventricular Wall for the Analysis of Ependymal Cell Cilia Organization
    小鼠脑室管膜细胞纤毛组织分析:脑室的解剖和染色
    作者:Paul Labedan, Cédric Matthews, Laurent Kodjabachian, Harold Cremer, Fadel Tissir and Camille Boutin日期:03/20/2016,浏览量:11777,Q&A: 0
    [Abstract] In the developing and mature central nervous system (CNS) the ventricular lumen is lined by the neuroepithelium and ependymal, respectively. These ventricular epithelia perform important functions related to the development, morphogenesis and physiology of the brain. In the mature CNS, ependyma constitutes a barrier between brain parenchyma and ...
    Chick Neural Tube Explant Culture
    鸡胚胎神经管外植体培养
    作者:Zhanna Alekseenko, Elisabet Andersson and José M. Dias日期:10/05/2015,浏览量:8681,Q&A: 0
    [Abstract] The neural tube explant culture technique allows in vitro culturing of small pieces of neural tissue isolated from e.g., chick or mouse embryonic tissue in a matrix of collagen for defined periods of time. This method can be used to study the effects of defined molecules on developmental processes such as neural progenitor ...