癌症生物学

分类

    现刊
    Generation of the Compression-induced Dedifferentiated Adipocytes (CiDAs) Using Hypertonic Medium
    使用高渗培养基压缩诱导的去分化脂肪细胞(CiDAs)的产生
    [Abstract]

    Current methods to obtain mesenchymal stem cells (MSCs) involve sampling, culturing, and expanding of primary MSCs from adipose, bone marrow, and umbilical cord tissues. However, the drawbacks are the limited numbers of total cells in MSC pools, and their decaying stemness during in vitro expansion. As an alternative resource, recent ceiling

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    Carboxyfluorescein Dye Uptake to Measure Connexin-mediated Hemichannel Activity in Cultured Cells
    用羧荧光素染料上染率测定培养细胞中连接蛋白介导的半通道活性
    作者:Joe A. Potter, Gareth W. Price, Chelsy L. Cliff, Bethany M. Williams, Claire E. Hills and Paul E. Squires日期:02/05/2021,浏览量:2762,Q&A: 0
    [Abstract]

    Connexins are membrane bound proteins that facilitate direct and local paracrine mediated cell-to-cell communication through their ability to oligomerise into hexameric hemichannels. When neighbouring channels align, they form gap-junctions that provide a direct route for information transfer between cells. In contrast to intact gap junctions,

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    A Transient Transfection-based Cell Adhesion Assay with 293T Cells
    基于瞬时转染的293T细胞粘附实验
    作者:Rohit Singh and Beom K. Choi日期:01/05/2021,浏览量:2571,Q&A: 0
    [Abstract]

    The in vitro cell adhesion assay is a quantitative method for measuring selective cell adhesion to specific proteins. Traditionally, cell adhesion assays employ purified protein immobilized on a solid glass or plastic surface. Here, we describe a transient 293T cell transfection-based cell adhesion assay to study selective cell adhesion of a

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    A Rigorous Quantitative Approach to Analyzing Phagocytosis Assays
    吞噬试验的严格定量分析方法
    作者:Michael D. Caponegro, Kaitlyn Koenig Thompson, Maryam Tayyab and Stella E. Tsirka日期:01/05/2021,浏览量:2360,Q&A: 0
    [Abstract] Studying monocytic cells in isolated systems in vitro contributes significantly to the understanding of innate immune physiology. Functional assays produce read outs which can be used to measure responses to selected stimuli, such as pathogen exposure, antigen loading, and cytokine stimulation. Integration of these results with high ...
    Triacylglycerol Measurement in HeLa Cells
    HeLa细胞中三酰甘油的测定
    作者:Ximing Du and Hongyuan Yang日期:12/20/2020,浏览量:1577,Q&A: 0
    [Abstract]

    Lipid droplets store triacylglycerols (triglycerides) and sterol esters to regulate lipid and energy homeostasis. Triacylglycerol measurement is often performed during the investigation of lipid droplet formation and growth. This protocol describes a reliable method using a fluorometric lipid quantification kit to measure triacylglycerols

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    Automated Analysis of Cell Surface Ruffling: Ruffle Quantification Macro
    细胞表面Ruffling的自动分析:Ruffle的宏观量化
    作者:Nicholas D. Condon, Jennifer L. Stow and Adam A. Wall日期:01/20/2020,浏览量:2662,Q&A: 1
    [Abstract] Cell surface protrusions include F-actin rich, wave-like ruffles that are erected transiently in response to stimuli and during cell migration. Macrophages are innate immune cells that ruffle constitutively and more dramatically in cells activated by pathogens. Dorsal ruffles and their resulting macropinosomes are key sites for environmental ...
    Immunofluorescence-based Determination of Centrosome Number in Tissue Samples
    基于免疫荧光法的组织样本中心体数目确定
    作者:Mengdie Wang, Gregory C. Rogers and Anne E. Cress日期:10/20/2019,浏览量:2887,Q&A: 0
    [Abstract] Centrosome numerical abnormalities have been reported in a variety of tumors. Centrosome numbers in cancer cells display both inter-tumor and intra-tumor heterogeneity. The over production of centrosomes (centrosome amplification) is unique in cancer cells and is a promising target for therapy. Thus, a method to quantify centrosome numbers on a ...
    RNA Interference Screening to Identify Proliferation Determinants in Breast Cancer Cells
    RNA干扰筛查鉴定乳腺癌细胞增殖的决定因素
    [Abstract] RNAi screening technology has revealed unknown determinants of various biological signaling pathways in biomedical studies. This protocol provided detailed information about how to use RNAi screening to identify proliferation determinants in breast tumor cells. siRNA-based libraries targeting against Estrogen receptor (ER)-network, including 631 ...
    Measurement of the Intracellular Calcium Concentration with Fura-2 AM Using a Fluorescence Plate Reader
    使用荧光读板仪通过钙指示剂Fura-2 AM 测定细胞内钙浓度
    作者:Magdiel Martínez, Namyr A. Martínez and Walter I. Silva日期:07/20/2017,浏览量:27016,Q&A: 1
    [Abstract] Intracellular calcium elevation triggers a wide range of cellular responses. Calcium responses can be affected or modulated by membrane receptors mutations, localization, exposure to agonists/antagonists, among others (Burgos et al., 2007; Martínez et al., 2016). Changes in intracellular calcium concentration can be measured ...
    Soft Agar Colony Formation Assay as a Hallmark of Carcinogenesis
    软琼脂集落形成实验测定作为致癌性标志的集落形成
    作者:Feng Du, Xiaodi Zhao and Daiming Fan日期:06/20/2017,浏览量:24636,Q&A: 0
    [Abstract] Soft agar colony formation assay is established to estimate the anchorage-independent growth ability of cells. In this assay, a bottom layer of agar with complete media is poured and solidified first, followed by an upper layer containing a specified number of cells suspended in medium-agar mixture. After two weeks of incubation, the number of ...