癌症生物学

分类

    现刊
    An Optimized Method to Isolate Human Fibroblasts from Tissue for Ex Vivo Analysis
    从组织中分离人成纤维细胞用于体外分析的优化方法
    [Abstract] Despite their involvement in many physiological and pathological processes, fibroblasts remain a poorly-characterized cell type. Analysis of primary fibroblasts while maintaining their in vivo phenotype is challenging: standard methods for fibroblast isolation require cell culture in vitro, which is known to alter phenotypes. ...
    A Cell Culture Model that Mimics Physiological Tissue Oxygenation Using Oxygen-permeable Membranes
    一种利用透氧膜模拟生理组织氧合的细胞培养模式
    作者:Chloe-Anne Martinez, Peter A. Cistulli and Kristina M. Cook日期:09/20/2019,浏览量:3562,Q&A: 0
    [Abstract] Dissolved oxygen and its availability to cells in culture is an overlooked variable which can have significant consequences on experimental research outcomes, including reproducibility. Oxygen sensing pathways play key roles in cell growth and behavior and pericellular oxygen levels should be controlled when establishing in vitro models. ...
    Generation of Tumour-stroma Minispheroids for Drug Efficacy Testing
    用于检测药物疗效的肿瘤基质小球体的生成
    作者:Mark Watters and Eva Szegezdi日期:01/05/2017,浏览量:6920,Q&A: 0
    [Abstract] The three-dimensional organisation of cells in a tissue and their interaction with adjacent cells and extracellular matrix is a key determinant of cellular responses, including how tumour cells respond to stress conditions or therapeutic drugs (Elliott and Yuan, 2011). In vivo, tumour cells are embedded in a stroma formed primarily by ...
    Preparation of Recombinant Galectin-3 for Cancer Studies
    用于癌症研究的重组半乳糖凝集素-3的制备
    作者:Kari Tyler, Sok-Hyong Lee and Erwin G. Van Meir日期:01/05/2016,浏览量:7555,Q&A: 0
    [Abstract] Galectin-3 is a member of a class of proteins termed Galectins, characterized by their ability to bind glycans containing β-galactose (Cummings and Liu, 2009). Galectin-3 binds preferentially to proteoglycans terminating with N-acetyllactosamine (LacNAc) chains (i.e., tandem repeats of galactose) (Newlaczyl and Yu, 2011). Galectin-3 is ...
    Three Dimensional Spheroid Co-culture Invasion Assay
    三维球状体共培养侵袭研究
    作者:Eldo T Verghese and Thomas A Hughes日期:01/05/2015,浏览量:11241,Q&A: 0
    [Abstract] The assay was developed to investigate the impact of stromal cells of different types (in our case breast cancer associated fibroblasts stably manipulated to modify expression of genes of interest) on the invasive capacity of epithelial cancer cells (in our case breast cancer cell lines) (Verghese et al., 2013). Typical two dimensional ...
    Three-dimensional Invasion Assay
    三维侵袭实验
    作者:Wen-Hao Yang and Muh-Hwa Yang日期:09/05/2013,浏览量:10307,Q&A: 0
    [Abstract] The invasive ability of cancer cells is a crucial function for cancer metastasis and the surrounding microenvironment of cancer cells in living tissues is three-dimension (3D). Therefore, to establish an in vitro invasion assay in a 3D system to predict cancer invasive ability is valuable in the research for cancer metastasis. Here, we ...