癌症生物学

分类

    现刊
    Measuring Cell Growth and Junction Development in Epithelial Cells Using Electric Cell-Substrate Impedance Sensing (ECIS)
    使用电子细胞基质阻抗传感(ECIS)测量上皮细胞生长和连接发育
    作者:Shaista Anwer and Katalin Szaszi日期:08/20/2020,浏览量:2474,Q&A: 0
    [Abstract] Electric Cell-substrate Impedance Sensing (ECIS) is an automated method that can be used to quantify processes such as cell attachment, growth, migration and barrier functions (i.e., the properties of tight junctions). The method provides simultaneous information on cell number and tight junction function by detecting electric parameters ...
    Imaging VIPER-labeled Cellular Proteins by Correlative Light and Electron Microscopy
    利用关联光学和电子显微镜技术进行VIPER标记细胞蛋白成像
    作者:Julia K. Doh, Young Hwan Chang, Caroline A. Enns, Claudia S. Lόpez and Kimberly E. Beatty日期:11/05/2019,浏览量:3343,Q&A: 0
    [Abstract] Advances in fluorescence microscopy (FM), electron microscopy (EM), and correlative light and EM (CLEM) offer unprecedented opportunities for studying diverse proteins and nanostructures involved in fundamental cell biology. It is now possible to visualize and quantify the spatial organization of cellular proteins and other macromolecules by FM, ...
    Implementing VIPER for Imaging Cellular Proteins by Fluorescence Microscopy
    VIPER用于荧光显微镜中细胞蛋白成像
    作者:Julia K. Doh, Caroline A. Enns and Kimberly E. Beatty日期:11/05/2019,浏览量:2982,Q&A: 0
    [Abstract] Genetically-encoded tags are useful tools for multicolor and multi-scale cellular imaging. Versatile Interacting Peptide (VIP) tags, such as VIPER, are new genetically-encoded tags that can be used in various imaging applications. VIP tags consist of a coiled-coil heterodimer, with one peptide serving as the genetic tag and the other (“probe ...
    Generation of CoilR Probe Peptides for VIPER-labeling of Cellular Proteins
    用于细胞蛋白VIPER标记的CoilR肽探针的制备
    作者:Julia K. Doh, Savannah J. Tobin and Kimberly E. Beatty日期:11/05/2019,浏览量:2872,Q&A: 0
    [Abstract] Versatile Interacting Peptide (VIP) tags are a new class of genetically-encoded tag designed for imaging cellular proteins by fluorescence and electron microscopy. In 2018, we reported the VIPER tag (Doh et al., 2018), which contains two elements: a genetically-encoded peptide tag (i.e., CoilE) and a probe peptide (i.e., ...
    Propidium Iodide Staining of Cells for FACS Analysis
    流式细胞术检测细胞增殖与凋亡(碘化丙啶法)
    作者:Hui Zhu日期:06/05/2012,浏览量:52235,Q&A: 2
    [Abstract] Fluorescence Activated Cell Sorting (FACS) is used to study DNA cell content. Propidium iodide (PI) intercalates into double-stranded nucleic acids and fluoresces. It is excluded by viable cells but can penetrate cell membranes of dying or dead cells. Thus PI staining is included in immunofluorescent staining protocols to identify dead cells. DNA ...