植物科学

分类

    现刊
    Analysis of Soluble Sugar Content in Minute Quantities of Rice Tissues by GC-MS
    气质联用技术分析水稻组织中微量可溶性糖含量
    作者:Li-he Xu, Li-ying Xiao, Xue-qiong Xiao, Yan-nong Xiao and Gao-feng Wang日期:07/05/2021,浏览量:1260,Q&A: 0
    [Abstract]

    Soluble sugars play key roles in plant growth, development, and adaption to the environment. Characterizing sugar content profiling of plant tissues promotes our understanding of the mechanisms underlying these plant processes. Several technologies have been developed to quantitate soluble sugar content in plant tissues; however, it is difficult

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    Pipecolic Acid Quantification Using Gas Chromatography-coupled Mass Spectrometry
    哌啶酸的气相色谱-质谱定量分析
    作者:Keshun Yu, Huazhen Liu and Pradeep Kachroo日期:12/05/2020,浏览量:1381,Q&A: 0
    [Abstract]

    Pipecolic acid (Pip), a non-proteinacious product of lysine catabolism, is an important regulator of immunity in plants and humans alike. For instance, Pip accumulation is associated with the genetic disorder Zellweger syndrome, chronic liver diseases, and pyridoxine-dependent epilepsy in humans. In plants, Pip accumulates upon pathogen infection

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    Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana
    甜菜夜蛾对拟南芥的取食试验
    作者:Yanrong You, Chunpeng An and Chuanyou Li日期:03/05/2020,浏览量:2277,Q&A: 0
    [Abstract] Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model ...
    Tracking Root Interactions System (TRIS) Experiment and Quality Control
    根相互作用系统的示踪实验和质量控制
    作者:Hassan Massalha, Elisa Korenblum, Orr H. Shapiro and Asaph Aharoni日期:04/20/2019,浏览量:4272,Q&A: 0
    [Abstract] Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high ...
    Quantification of the Humidity Effect on HR by Ion Leakage Assay
    离子渗漏实验定量分析湿度对超敏反应的影响
    作者:Musoki Mwimba and Xinnian Dong日期:04/05/2019,浏览量:3296,Q&A: 0
    [Abstract] We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the ...
    Peptide Feeding and Mechanical Wounding for Tomato Seedlings
    番茄幼苗的肽饲喂和机械伤害
    作者:Lei Wang, Yan Wang and Georg Felix日期:03/20/2019,浏览量:3699,Q&A: 0
    [Abstract] Plants need to respond appropriately to wounding and herbivorous insects. Peptide signals have been implicated in local and systemic induction of appropriate plant defense responses. To study these peptide signals and their perception in host plants, it is important to have reproducible bioassays. Several assays, such as treatment of peptide ...
    Real-time PCR Analysis of PAMP-induced Marker Gene Expression in Nicotiana benthamiana
    本塞姆氏烟草中PAMP诱导标记基因表达的实时定量PCR分析
    作者:Fan Liu, Yuanpeng Xu, Yan Wang and Yuanchao Wang日期:10/05/2018,浏览量:5316,Q&A: 0
    [Abstract] Perception of pathogen-associated molecular patterns (PAMPs) often triggers various innate immune responses in plants. The transcriptional changes of defense-related genes are often used as a marker to assay PAMP-triggered plant immune response. Here we described a protocol to monitor the relative expression level of marker genes in Nicotiana ...
    Transient Expression Assay in NahG Arabidopsis Plants Using Agrobacterium tumefaciens
    使用根癌农杆菌在NahG拟南芥植株中进行瞬时表达分析
    作者:Pepe Cana-Quijada, Eduardo R. Bejarano, Rosa Lozano-Durán and Tábata Rosas-Díaz日期:06/20/2018,浏览量:6357,Q&A: 1
    [Abstract] Agrobacterium-mediated transient expression has greatly contributed to research in molecular plant biology but has low efficiency and inconsistency in Arabidopsis thaliana (Arabidopsis). Here, we describe a simple, efficient and fast protocol to make transient gene expression in NahG Arabidopsis plants using ...
    Quantification of Thrips Damage Using Ilastik and ImageJ Fiji
    用Ilastik及ImageJ Fiji定量蓟马危害
    作者:Isabella G. S. Visschers, Nicole M. van Dam and Janny L. Peters日期:04/20/2018,浏览量:7124,Q&A: 0
    [Abstract] Quantification of insect damage is an essential measurement for identifying resistance in plants. In screening for host plant resistance against thrips, the total damaged leaf area is used as a criterion to determine resistance levels. Here we present an objective novel method for analyzing thrips damage on leaf disc using the freely available ...
    Haustorium Induction Assay of the Parasitic Plant Phtheirospermum japonicum
    寄生植物日本松蒿的吸器诱导测定
    作者:Juliane K. Ishida, Satoko Yoshida and Ken Shirasu日期:05/05/2017,浏览量:7167,Q&A: 0
    [Abstract] Phtheirospermum japonicum is a facultative root parasitic plant in the Orobanchaceae family used as a model parasitic plant. Facultative root parasites form an invasive organ called haustorium on the lateral parts of their roots. To functionally characterize parasitic abilities, quantification of haustorium numbers is required. However, ...