细胞生物学

分类

    现刊
    A Versatile Protocol to Quantify BCR-mediated Phosphorylation in Human and Murine B Cell Subpopulations
    量化人类和小鼠B细胞亚群中BCR介导的磷酸化的通用实验方法
    作者:Jasper Rip, Rudi W. Hendriks and Odilia B. J. Corneth日期:02/05/2021,浏览量:805,Q&A: 0
    [Abstract]

    Signal transduction is the process by which molecular signals are transmitted from the cell surface to its interior, resulting in functional changes inside the cell. B cell receptor (BCR) signaling is of crucial importance for B cells, as it regulates their differentiation, selection, survival, cellular activation and proliferation. Upon BCR

    ...
    Quantification of the Surface Expression of G Protein-coupled Receptors Using Intact Live-cell Radioligand Binding Assays
    利用完整活细胞放射配体结合分析定量G蛋白偶联受体的表面表达
    作者:Xin Xu and Guangyu Wu日期:09/20/2020,浏览量:2153,Q&A: 0
    [Abstract] G protein-coupled receptors (GPCRs) are the most structurally diverse family of signaling proteins and regulate a variety of cell function. For most GPCRs, the cell surface is their functional destination where they are able to respond a wide range of extracellular stimuli, leading to the activation of intracellular signal transduction cascades. ...
    Flow-cytometric Detection of Low-level Reactive Oxygen Species in Cell Lines and Primary Immune Cells
    细胞系和原代免疫细胞中的低水平活性氧的流式细胞仪检测
    作者:Kevin Bode, Corinna Link, Peter H. Krammer and Heiko Weyd日期:09/05/2020,浏览量:2919,Q&A: 0
    [Abstract] Depending on its concentration and cellular origin the production of reactive oxygen species (ROS) in the organism serves a variety of functions. While high concentrations during an oxidative burst are used to fight pathogens, low to moderate amounts of ROS act as signaling molecules important for several physiological processes such as regulation ...
    Assessing Gαq/15-signaling with IP-One: Single Plate Transfection and Assay Protocol for Cell-Based High-Throughput Assay
    使用IP-One评估Gαq/ 15信号:基于细胞高通量测定的单板转染和测定方案
    作者:Élie Besserer-Offroy, Rebecca L Brouillette, Jean-Michel Longpré and Philippe Sarret日期:08/20/2020,浏览量:1816,Q&A: 0
    [Abstract] Cell-based functional assays are an important part of compound screening and drug lead optimization, and they can also play a crucial role in the determination of the residues involved in ligand binding and signaling for a particular G-protein-coupled receptor. Conventional methods used for Gαq/15-coupled receptors rely on the use of ...
    An ex vivo Approach to Assess Mitochondrial ROS by Flow Cytometry in AAV-tagged Astrocytes in Adult Mice
    一种利用流式细胞术进行成年大鼠腺相关病毒标记星型胶质细胞中线粒体ROS水平的体外检测方法
    作者:Carlos Vicente-Gutierrez and Juan P. Bolaños日期:03/20/2020,浏览量:2627,Q&A: 0
    [Abstract] Mitochondrial reactive oxygen species (mROS) are naturally produced signalling molecules extremely relevant for understanding both health- and disease-associated biological processes. The study of mROS in the brain is currently underway to decipher their physiopathological roles and contributions in neurological diseases. Recent advances in this ...
    Tandem Affinity Purification of SBP-CBP-tagged Type Three Secretion System Effectors
    SBP-CBP-tagged三型分泌系统效应因子的串联亲和纯化
    作者:Laura Berneking, Marie Schnapp, Theresa Nauth and Moritz Hentschke日期:06/20/2019,浏览量:2954,Q&A: 0
    [Abstract] Identification of protein-protein interactions of bacterial effectors and cellular targets during infection is at the core to understand how bacteria manipulate the infected host to overcome the immune response. Potential interacting proteins might be identified by genetic methods, i.e., two hybrid screens and could be verified by ...
    Cell Synchronization by Double Thymidine Block
    用胸腺嘧啶核苷双阻断法同步细胞
    作者:Guo Chen and Xingming Deng日期:09/05/2018,浏览量:24162,Q&A: 1
    [Abstract] Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. Here, we present the protocol to synchronize cells at ...
    An in vitro Co-culture System for the Activation of CD40 by Membrane-presented CD40 Ligand versus Soluble Agonist
    细胞膜呈递CD40配体与可溶性激动剂激活CD40的体外共培养系统
    [Abstract] One fundamental property of the TNR receptor (TNFR) family relates to how ‘signal quality’ (the extent of receptor ligation or cross-linking) influences the outcome of receptor ligation, for instance the induction of death in tumour cells. It is unequivocal that membrane-presented ligand (delivered to target cells via cell-surface presentation by ...
    3D Culture Protocol for Testing Gene Knockdown Efficiency and Cell Line Derivation
    用于检测基因敲减效率和细胞系建立3D培养方法
    作者:Jan Strnadel, Sang Myung Woo, Sunkyu Choi, Huawei Wang, Marian Grendar and Ken Fujimura日期:06/05/2018,浏览量:7219,Q&A: 0
    [Abstract] Traditional 2D cell cultures with cells grown as monolayers on solid surface still represent the standard method in cancer research for drug testing. Cells grown in 2D cultures, however, lack relevant cell-matrix and cell-cell interactions and ignore the true three-dimensional anatomy of solid tumors. Cells cultured in 2D can also undergo ...
    Measurement of Intracellular ROS in Caenorhabditis elegans Using 2’,7’-Dichlorodihydrofluorescein Diacetate
    使用2',7'-二氯二氢荧光素二乙酸盐测定秀丽隐杆线虫细胞内ROS
    作者:Dong Suk Yoon, Myon-Hee Lee and Dong Seok Cha日期:03/20/2018,浏览量:10254,Q&A: 1
    [Abstract] Reactive oxygen species (ROS) are generated during normal metabolic processes under aerobic conditions. Since ROS production initiates harmful radical chain reactions on cellular macromolecules, including lipid peroxidation, DNA mutation, and protein denaturation, it has been implicated in a wide spectrum of diseases such as cancer, ...