分子生物学

分类

    现刊
    Immunohistochemistry of Kidney a-SMA, Collagen 1, and Collagen 3, in A Novel Mouse Model of Reno-cardiac Syndrome
    心肾综合征新型小鼠模型中肾脏a-SMA,胶原1和胶原3的免疫组化研究
    [Abstract] Cardiorenal syndrome defines a synergistic pathology of the heart and kidneys where failure of one organ causes failure in the other. The incidence of cardiovascular mortality caused by this syndrome, is 20 fold higher in the end stage renal disease (ESRD) population compared to the population as a whole thus necessitating the need for improved ...
    Electrophoretic Mobility Shift Assay of in vitro Phosphorylated RNA Polymerase II Carboxyl-terminal Domain Substrates
    体外磷酸化的RNA聚合酶II羧基末端域底物的电泳迁移率测定
    作者:Joshua E. Mayfield, Seema Irani and Yan Zhang日期:06/20/2020,浏览量:2123,Q&A: 0
    [Abstract] Eukaryotic RNA polymerase II transcribes all protein-coding mRNAs and is highly regulated. A key mechanism directing RNA polymerase II and facilitating the co-transcriptional processing of mRNAs is the phosphorylation of its highly repetitive carboxyl-terminal domain (CTD) of its largest subunit, RPB1, at specific residues. A variety of techniques ...
    An in vitro Assay to Screen for Substrates of PKA Using Phospho-motif-specific Antibodies
    利用磷酸基团特异性抗体体外筛选PKA底物
    作者:David M. Gau and Partha Roy日期:04/20/2020,浏览量:1646,Q&A: 0
    [Abstract] Kinases function as regulators of many cellular processes such as cell migration. These enzymes typically phosphorylate target motif sequences. Mass spec or phospho-specific antibody detection can be used to determine whether a kinase can phosphorylate proteins of interest, however, mass spec can be expensive and phospho-antibodies for the protein ...
    A Radioactive in vitro ERK3 Kinase Assay
    体外ERK3激酶的放射性分析
    作者:Lobna Elkhadragy and Weiwen Long日期:08/20/2019,浏览量:3508,Q&A: 0
    [Abstract] Mitogen-activated protein kinases (MAPKs) are serine/threonine kinases that have an important role in signal transduction. Extracellular signal-regulated kinase 3 (ERK3), also known as MAPK6, is an atypical MAPK. Here, we describe in detail an in vitro assay for the kinase activity of ERK3 using myelin basic protein (MBP) or steroid ...
    Unbiased Screening of Activated Receptor Tyrosine Kinases (RTKs) in Tumor Extracts Using a Mouse Phospho-RTK Array Kit
    利用小鼠Phospho-RTK Array试剂盒无偏筛选肿瘤提取物中激活受体酪氨酸激酶
    作者:Julian Naipauer, Lucas E. Cavallin and Enrique A. Mesri日期:04/20/2019,浏览量:3150,Q&A: 0
    [Abstract] Kaposi’s sarcoma (KS) herpesvirus (KSHV) is a virus that causes KS, an angiogenic AIDS-associated spindle-cell neoplasm, by activating host oncogenic signaling cascades through autocrine and paracrine mechanisms. Many host signaling cascades co-opted by KSHV including PI3K/AKT/mTORC, NFkB and Notch are critical for cell-specific mechanisms of ...
    Protocol for in situ Proximity Ligation Assay (PLA) and Microscopy Analysis of Epidermal Growth Factor Receptor (EGFR) Homodimerization
    原位邻位连接检测和显微镜分析表皮生长因子的同源二聚化的实验方法
    作者:Keiichi Ota and Taishi Harada日期:11/05/2018,浏览量:5277,Q&A: 0
    [Abstract] Oncogenic drivers play central roles in tumorigenesis as well as in tumor cell survival and proliferation. Mutations of the epidermal growth factor receptor gene (EGFR) that result in constitutive activation of the receptor tyrosine kinase have been identified as oncogenic drivers in a subset of non-small cell lung cancer (NSCLC). ...
    In vitro Autophosphorylation and Phosphotransfer Assay of Cyanobacterial Histidine Kinase 2
    蓝藻组胺酸激酶2的体外自磷酸化和磷酸根传递转分析
    作者:Iskander M. Ibrahim日期:12/05/2016,浏览量:8834,Q&A: 0
    [Abstract] This is a detailed protocol of an autophosphorylation and phosphotransfer activities of Synechocystis sp. PCC 6803 full-length Histidine Kinase 2 (Hik2) protein described by Ibrahim et al., 2016. In this protocol, radioactively labelled ATP was used to study an autophosphorylation and phosphotransfer activity of the full-length ...