植物科学

分类

    现刊
    Tethered Chromosome Conformation Capture Sequencing in Triticeae: A Valuable Tool for Genome Assembly
    小麦族系中染色体构象捕获测序:一个有价值的基因组组装工具
    作者:Axel Himmelbach, Ines Walde, Martin Mascher and Nils Stein日期:08/05/2018,浏览量:3576,Q&A: 0
    [Abstract] Chromosome conformation capture sequencing (Hi-C) is a powerful method to comprehensively interrogate the three-dimensional positioning of chromatin in the nucleus. The development of Hi-C can be traced back to successive increases in the resolution and throughput of chromosome conformation capture (3C) (Dekker et al., 2002). The basic ...
    DNA-free Genome Editing of Chlamydomonas reinhardtii Using CRISPR and Subsequent Mutant Analysis
    利用CRISPR技术对莱茵衣藻进行DNA-free的基因组编并对产生突变进行分析
    作者:Jihyeon Yu, Kwangryul Baek, EonSeon Jin and Sangsu Bae日期:06/05/2017,浏览量:8206,Q&A: 0
    [Abstract] We successfully introduced targeted knock-out of gene of interest in Chlamydomonas reinhardtii by using DNA-free CRISPR. In this protocol, the detailed procedures of an entire workflow cover from the initial target selection of CRISPR to the mutant analysis using next generation sequencing (NGS) technology. Furthermore, we introduce a ...
    Chromosome Dosage Analysis in Plants Using Whole Genome Sequencing
    采用全基因组测序法进行染色体剂量分析
    作者:Ek Han Tan, Luca Comai and Isabelle M. Henry日期:07/05/2016,浏览量:7197,Q&A: 0
    [Abstract] Relative chromosome dosage, i.e., increases or decreases in the number of copies of specific chromosome regions in one sample versus another, can be determined using aligned read-counts from Illumina sequencing (Henry et al., 2010). The following protocol was used to identify the different classes of aneuploids that ...
    Protocol for the Generation of a Transcription Factor Open Reading Frame Collection (TFome)
    转录因子开放阅读框收集(Tfome)的生成方法
    [Abstract] The construction of a physical collection of open reading frames (ORFeomes) for genes of any model organism is a useful tool for the exploration of gene function, gene regulation, and protein-protein interaction. Here we describe in detail a protocol that has been used to develop the first collection of transcription factor (TF) and co-regulator ...
    Whole Genome Bisulfite Sequencing and DNA Methylation Analysis from Plant Tissue
    植物组织全基因组的亚硫酸盐测序和DNA甲基化分析
    作者:Daniela Pignatta, George W. Bell and Mary Gehring日期:02/20/2015,浏览量:14439,Q&A: 0
    [Abstract] This protocol describes whole genome bisulfite-sequencing library preparation from plant tissue and subsequent data analysis. Allele-specific methylation analysis and genome-wide identification of differentially methylated regions are additional features of the analysis procedure.
    Plant Sequence Capture Optimised for Illumina Sequencing
    优化用于Illumina测序的植物序列捕获技术
    作者:Axel Himmelbach, Manuela Knauft and Nils Stein日期:07/05/2014,浏览量:11123,Q&A: 0
    [Abstract] Plant Sequence Capture is used for targeted resequencing of whole exomes (all exons of a genome) of complex genomes e.g. barley and its relatives (Mascher et al., 2013). Sequencing and computing costs are significantly reduced since only the greatly enriched and gene-coding part of the barley genome is targeted, that corresponds ...
    Library Construction for Genome-wide Bisulfite Sequencing in Plants
    拟南芥Bs-seq文库的构建
    作者:David Moraga Amador, Chenggang Wang, Kevin H. Holland and Zhonglin Mou日期:12/20/2013,浏览量:10521,Q&A: 0
    [Abstract] DNA methylation is the most studied epigenetic modification, which involves the addition of a methyl group to the carbon-5 position of cytosine residues in DNA. DNA methylation is important for the regulation of gene expression. Bisulfite sequencing is the gold standard technique for determining genome-wide DNA methylation profiles in eukaryotes. ...