微生物学

分类

    现刊
    Generation and Implementation of Reporter BHK-21 Cells for Live Imaging of Flavivirus Infection
    黄病毒感染活体显像报告细胞BHK-21的制备与实施
    作者:Jorge L. Arias-Arias and Rodrigo Mora-Rodríguez日期:03/05/2021,浏览量:1116,Q&A: 0
    [Abstract]

    The genus Flavivirus within the family Flaviviridae includes many viral species of medical importance, such as yellow fever virus (YFV), Zika virus (ZIKV), and dengue virus (DENV), among others. Presently, the identification of flavivirus-infected cells is based on either the immunolabeling of viral proteins, the application of recombinant

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    TZA, a Sensitive Reporter Cell-based Assay to Accurately and Rapidly Quantify Inducible, Replication-competent Latent HIV-1 from Resting CD4+ T Cells
    TZA, 一种基于敏感报告因子细胞学检测方法, 可用于准确快速量化静息CD4+ T细胞中可诱导的且复制能力强的潜伏HV-1
    作者:Anwesha Sanyal, Vatsala S. Rangachar and Phalguni Gupta日期:05/20/2019,浏览量:4964,Q&A: 1
    [Abstract] The latent HIV-1 viral reservoir in resting CD4+ (rCD4+) T cells represents a major barrier to an HIV-1 cure. There is an ongoing effort to identify therapeutic approaches that will eliminate or reduce the size of this reservoir. However, clinical investigators lack an assay to determine whether or not a decrease in the ...
    In vivo Analysis of Cyclic di-GMP Cyclase and Phosphodiesterase Activity in Escherichia coli Using a Vc2 Riboswitch-based Assay
    使用基于Vc2 Riboswitch的测定法体内分析大肠杆菌中环状di-GMP环化酶和磷酸二酯酶活性
    作者:Ying Liu, Hyunhee Kim and Ute Römling日期:03/05/2018,浏览量:5450,Q&A: 0
    [Abstract] Cyclic di-guanosine monophosphate (c-di-GMP) is a ubiquitous second messenger that regulates distinct aspects of bacterial physiology. It is synthesized by diguanylate cyclases (DGCs) and hydrolyzed by phosphodiesterases (PDEs). To date, the activities of DGC and PDE are commonly assessed by phenotypic assays, mass spectrometry analysis of ...
    Isolation, BODIPY Labeling and Uptake of Exosomes in Hepatic Stellate Cells
    肝星状细胞中外泌体的分离、BODIPY标记和摄取
    作者:Shubham Shrivastava日期:12/05/2017,浏览量:8679,Q&A: 1
    [Abstract] Exosomes have emerged as an important mediator of intercellular communication. They are present in extracellular milieu and therefore, easily accessible by neighboring or distant cells. They carry mRNA, microRNAs and proteins within their vesicles and once internalized by recipient cells; they can modulate multiple signaling pathways with ...
    In vitro Assays for Measuring Endothelial Permeability by Transwells and Electrical Impedance Systems
    利用Transwell试验和电阻抗系统体外测量内皮通透性
    作者:Hong-Ru Chen and Trai-Ming Yeh日期:05/05/2017,浏览量:21433,Q&A: 2
    [Abstract] Vascular leakage is an important feature in several diseases, such as septic shock, viral hemorrhagic fever, cancer metastasis and ischemia-reperfusion injuries. Thus establishing assays for measuring endothelial permeability will provide insight into the establishment or progression of such diseases. Here, we provide transwell permeability assay ...
    Reporter Assay for Semen-mediated Enhancement of HIV-1 Infection
    报告基因试验法研究精液介导增强HIV-1感染
    作者:Janis A. Müller and Jan Münch日期:07/20/2016,浏览量:8480,Q&A: 0
    [Abstract] Semen contains amyloid fibrils that enhance HIV-1 infection (Münch et al., 2007; Kim et al., 2010; Roan et al., 2011; Arnold et al., 2012; Usmani et al., 2014; Roan et al., 2014). Positively charged semen amyloids capture negatively charged viral particles and increase their attachment rates to ...
    High-throughput β-galactosidase and β-glucuronidase Assays Using Fluorogenic Substrates
    利用荧光底物高通量分析β半乳糖苷酶和β葡萄糖醛酸酶
    作者:Joshua P. Ramsay日期:07/20/2013,浏览量:13517,Q&A: 0
    [Abstract] β-galactosidase and β-glucuronidase enzymes are commonly used as reporters for gene expression from gene promoter-lacZ or uidA fusions (respectively). The protocol described here is a high-throughput alternative to the commonly used Miller assay (Miller, 1972) that utilises a fluorogenic substrate (Fiksdal et al., 1994) ...