癌症生物学

分类

    现刊
    Measuring Protein Synthesis during Cell Cycle by Azidohomoalanine (AHA) Labeling and Flow Cytometric Analysis
    利用叠氮丙氨酸标记和流式细胞分析测定细胞周期中蛋白的合成
    作者:Koshi Imami and Tomoharu Yasuda日期:04/20/2019,浏览量:5621,Q&A: 0
    [Abstract] Protein synthesis is one of the most fundamental biological processes to maintain cellular proteostasis. Azidohomoalaine (AHA) is a non-radioactive and “clickable” amino acid analog of methionine which can be incorporated into newly synthesized proteins. Thus, AHA-labeled nascent proteins can be detected and quantified through fluorescent labeling ...
    Analyzing the Properties of Murine Intestinal Mucins by Electrophoresis and Histology
    电泳和组织学检查分析小鼠肠黏蛋白的性质
    作者:Ran Wang and Sumaira Z. Hasnain日期:07/20/2017,浏览量:15559,Q&A: 0
    [Abstract] Specialized secretory cells known as goblet cells in the intestine and respiratory epithelium are responsible for the secretion of mucins. Mucins are large heavily glycosylated proteins and typically have a molecular mass higher than 106 Da. These large proteins are densely substituted with short glycan chains, which have many important ...
    In vitro Assessment of RNA Polymerase I Activity
    RNA聚合酶I活性的体外评估
    作者:Marzia Govoni日期:02/05/2017,浏览量:6577,Q&A: 0
    [Abstract] In eukaryotic cells transcriptional processes are carried out by three different RNA polymerases: RNA polymerase I which specifically transcribes ribosomal RNA (rRNA), RNA polymerase II which transcribes protein-coding genes to yield messenger RNAs (mRNAs) and small RNAs, while RNA polymerase III transcribes the genes for transfer RNAs and for the ...
    Total Histone Acid Extraction of Colon Cancer HCT116 Cells
    酸性法提取结肠癌细胞HCT116的组蛋白
    作者:Lin-Lin Cao and Wei-Guo Zhu日期:11/20/2016,浏览量:8853,Q&A: 0
    [Abstract] Histone acid extraction assay is a popular method to determine histone modification levels in mammalian cells. It includes three steps: first, histones are released from chromatin by sulfuric acid; trichloroacetate (TCA) is then added to precipitate histones; and finally, histones are dissolved in double-distilled H2O (ddH2 ...
    Cell Surface Protein Detection to Assess Receptor Internalization
    细胞表面蛋白检测法检测受体的内化
    作者:Magdalena Czarnecka and Joanna Kitlinska日期:10/20/2016,浏览量:8159,Q&A: 0
    [Abstract] The migration of membrane receptors upon exposure to different stimulants/inhibitors is of great importance. Among others, the internalization of membrane receptors affects their accessibility to ligands and cell responsiveness to environmental cues. Experimentally, receptor internalization can be used as a measure of their activation. In our ...
    Preparation of Recombinant Galectin-3 for Cancer Studies
    用于癌症研究的重组半乳糖凝集素-3的制备
    作者:Kari Tyler, Sok-Hyong Lee and Erwin G. Van Meir日期:01/05/2016,浏览量:7694,Q&A: 0
    [Abstract] Galectin-3 is a member of a class of proteins termed Galectins, characterized by their ability to bind glycans containing β-galactose (Cummings and Liu, 2009). Galectin-3 binds preferentially to proteoglycans terminating with N-acetyllactosamine (LacNAc) chains (i.e., tandem repeats of galactose) (Newlaczyl and Yu, 2011). Galectin-3 is ...
    Proximity Ligation Assay (PLA) to Detect Protein-protein Interactions in Breast Cancer Cells
    邻位连接技术(PLA)检测乳腺癌细胞中蛋白质的相互作用
    作者:Mike Z Lin, Janet L Martin and Robert C Baxter日期:05/20/2015,浏览量:27676,Q&A: 0
    [Abstract] Protein-protein interaction networks provide a global picture of cellular function and biological processes, and the dysfunction of some interactions causes many diseases, including cancer. The in situ proximity ligation assay (PLA) is a powerful technology capable of detecting the interactions among proteins in fixed tissue and cell ...
    Detection of Phospho-KRAS by Electrophoretic Mobility Change in Human Cell Lines and in Tumor Samples from Nude Mice Grafts
    利用电泳迁移率的变化检测人源细胞系和裸小鼠移植肿瘤样本中的KRAS磷酸化
    作者:Carles Barceló, Noelia Paco, Debora Cabot, Eduard Garrido, Neus Agell and Montserrat Jaumot日期:03/20/2015,浏览量:8340,Q&A: 0
    [Abstract] KRAS is the oncogene most frequently mutated in human solid tumors especially in pancreas, colon, small intestine, biliary tract and lung. We have recently demonstrated that oncogenic KRAS needs S181 phosphorylation to fully display its oncogenic features suggesting its inhibition as a therapeutic treatment against KRAS-driven tumors. Due to the ...
    Analysis of Protein Stability by the Cycloheximide Chase Assay
    放线菌酮追踪检测分析蛋白质的稳定性
    [Abstract] Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly used in cell biology in terms of determining the half-life of a given protein and has gained much popularity in cancer research. Here we present a ...
    Hypoxia Studies with Pimonidazole in vivo
    体内哌莫硝唑缺氧研究
    作者:Kristina Y. Aguilera and Rolf A. Brekken日期:10/05/2014,浏览量:22784,Q&A: 0
    [Abstract] Therapy-induced hypoxia drives changes in the tumor microenvironment that contribute to the poor response to therapy. Hypoxia is capable of driving the expression and/or activation of specific signaling cascades (e.g., c-Met, Axl, CTGF), the recruitment of tumor promoting immune cells, and the induction of cell survival pathways including ...