生物化学

分类

    现刊
    Chromatin Immunoprecipitation (ChIP) Assay for Detecting Direct and Indirect Protein – DNA Interactions in Magnaporthe oryzae
    利用染色质免疫共沉淀(ChIP)技术检测稻瘟病菌中蛋白质-DNA的直接与间接相互作用
    作者:Gang Li, Margarita Marroquin-Guzman and Richard A. Wilson日期:11/05/2015,浏览量:12194,Q&A: 0
    [Abstract] Chromatin immunoprecipitation (ChIP) is a powerful technology for analyzing protein-DNA interactions in cells. Robust ChIP procedures have been established for investigating direct interactions between protein and DNA. However, detecting indirect protein-DNA interactions in vivo is challenging. Recently, we used ChIP to analyze an ...
    RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans
    秀丽隐杆线虫的RNA染色质免疫共沉淀(RNA-ChIP)
    作者:Germano Cecere and Alla Grishok日期:12/20/2014,浏览量:12387,Q&A: 0
    [Abstract] The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA–protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA–protein complexes. Here we describe the RNA–ChIP protocol that we have adapted for Caenorhabditis elegans (C. elegans ...
    ChIP-Seq in Candida albicans
    白色念珠菌的染色质免疫共沉淀测序(ChIP-Seq)
    [Abstract] Systems biology approaches can be used to study the regulatory interactions occurring between many components of the biological system at the whole-genome level and decipher the circuitries implicated in the regulation of cellular processes, including those imparting virulence to opportunistic fungi. Candida albicans (C. albicans ...
    Identification of Proteins Interacting with Genomic Regions of Interest in vivo Using Engineered DNA-binding Molecule-mediated Chromatin Immunoprecipitation (enChIP)
    体内使用DNA结合分子介导染色质免疫沉淀法(enChIP)对于与目的基因组相互作用的蛋白质进行识别
    作者:Toshitsugu Fujita and Hodaka Fujii日期:05/20/2014,浏览量:12255,Q&A: 0
    [Abstract] Elucidation of molecular mechanisms of genome functions requires identification of molecules interacting with genomic regions of interest in vivo. To this end, it is useful to isolate the target regions retaining molecular interactions. We established locus-specific chromatin immunoprecipitation (ChIP) technologies consisting of ...
    Chromatin Immunoprecipitation (ChIP), Streptavidin and ATP-agarose Mediated Pull-down Analyses
    染色质免疫共沉淀、链酶亲和素和琼脂糖三磷酸腺苷介导的Pull-down检测技术
    [Abstract] Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) induces expression of both viral and cellular genes in virus infected B cells by mimicking activated Notch receptors (Notch-IC) that mediate transcription activation through binding to the repressing domain of the recombining binding protein suppressor of hairless (RBP-Jκ). In general, chromatin ...
    Estradiol Receptor (ER) Chromatin Immunoprecipitation in MCF-7 Cells
    MCF-7 细胞中的雌激素受体(ER)染色质免疫共沉淀
    作者:Pia Giovannelli, Gabriella Castoria and Antimo Migliaccio日期:07/20/2013,浏览量:8944,Q&A: 0
    [Abstract] Steroid hormone receptors, for example estradiol receptor, act like transcription factors. In the cell, steroids bind to a specific receptor. Upon ligand binding, many steroid receptors dimerize and enter nuclei where they bind specific DNA sequences called Hormone Responsive Elements (HRE) and regulate gene transcription. ER is able to bind DNA ...
    p65 Chromatin Immunoprecipitation Protocol
    p65染色质免疫共沉淀法
    作者:Crissy Dudgeon日期:04/20/2013,浏览量:9951,Q&A: 0
    [Abstract] Chromatin Immunoprecipitation (ChIP) is an important procedure that allows you to verify if a certain protein is physically located at a regulatory region. This information, taken together with other procedures such as luciferase assays and EMSAs, will give definitive proof that the query protein is involved in the transcription of a protein. ...
    Illumina Sequencing Library Construction from ChIP DNA
    将ChIP方法获取的DNA用来构建Illumina测序文库
    作者:Wei Zheng日期:02/20/2012,浏览量:18660,Q&A: 0
    [Abstract] The Illumina sequencing platform is very popular among next-generation sequencing platforms. However, the DNA sequencing library construction kit provided by Illumina is considerably expensive. The protocol described here can be used to construct high-quality sequencing libraries from chromatin immunoprecipitated DNA. It uses key reagents from ...