微生物学

分类

    现刊
    Differential Fractionation of Erythrocytes Infected by Plasmodium berghei
    感染伯氏疟原虫红细胞的差异分离
    作者:Bénédicte Gnangnon, Véronique Peucelle and Christine Pierrot日期:06/05/2020,浏览量:1574,Q&A: 0
    [Abstract] The study of host/pathogen interactions at the cellular level during Plasmodium intra-erythrocytic cycle requires differential extraction techniques aiming to analyze the different compartments of the infected cell. Various protocols have been proposed in the literature to study specific compartments and/or membranes in the infected ...
    Isolation of Thylakoid Membranes from the Cyanobacterium Synechocystis sp. PCC 6803 and Analysis of Their Photosynthetic Pigment-protein Complexes by Clear Native-PAGE
    从蓝藻集胞藻sp. PCC 6803中分离类囊体膜并利用CN-PAGE非变性聚丙烯酰胺凝胶电泳分析光合色素蛋白复合物
    作者:Josef Komenda, Vendula Krynická and Tomas Zakar日期:01/05/2019,浏览量:4670,Q&A: 0
    [Abstract] Cyanobacteria represent a frequently used model organism for the study of oxygenic photosynthesis. They belong to prokaryotic microorganisms but their photosynthetic apparatus is quite similar to that found in algal and plant chloroplasts. The key players in light reactions of photosynthesis are Photosystem I and Photosystem II complexes (PSI and ...
    Selective Isolation of Retroviruses from Extracellular Vesicles by Intact Virion Immunoprecipitation
    通过完整病毒免疫沉淀技术从细胞外囊泡选择性分离逆转录病毒
    作者:Tyler Milston Renner, Kasandra Bélanger and Marc-André Langlois日期:09/05/2018,浏览量:4375,Q&A: 0
    [Abstract] There exists a wide variety of techniques to isolate and purify viral particles from cell culture supernatants. However, these techniques vary greatly in ease of use, purity, yield and impact on viral structural integrity. Most importantly, it is becoming evident that secreted extracellular vesicles (EVs) co-purify with retroviruses using nearly ...
    Easy and Efficient Permeabilization of Cyanobacteria for in vivo Enzyme Assays Using B-PER
    使用B-PER简单有效地透化蓝藻以进行体内酶分析
    作者:Simon Matthé Erstad and Yumiko Sakuragi日期:01/05/2018,浏览量:5168,Q&A: 0
    [Abstract] Cyanobacteria are photosynthetic bacteria that thrive in diverse ecosystems and play major roles in the global carbon cycle. The abilities of cyanobacteria to fix atmospheric CO2 and to allocate the fixed carbons to chemicals and biofuels have attracted growing attentions as sustainable microbial cell factories. A better understanding ...
    Expression and Purification of the Cas10-Csm Complex from Staphylococci
    表达并纯化葡萄球菌的Cas10-Csm复合体
    作者:Lucy Chou-Zheng and Asma Hatoum-Aslan日期:06/05/2017,浏览量:8139,Q&A: 0
    [Abstract] CRISPR-Cas (Clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) is a class of prokaryotic immune systems that degrade foreign nucleic acids in a sequence-specific manner. These systems rely upon ribonucleoprotein complexes composed of Cas nucleases and small CRISPR RNAs (crRNAs). Staphylococcus epidermidis ...
    Isolation of Ribosomal Particles from the Unicellular Cyanobacterium Synechocystis sp. PCC 6803
    从单细胞蓝细菌集胞藻PCC 6803 中分离核糖体颗粒
    作者:Carla V. Galmozzi and M. Isabel Muro-Pastor 日期:03/20/2017,浏览量:6053,Q&A: 0
    [Abstract] Isolation of ribosomal particles is an essential step in the study of ribosomal components as well as in the analysis of trans-acting factors that interact with the ribosome to regulate protein synthesis and modulate the expression profile of the cell in response to different environmental conditions. In this protocol, we describe a ...
    Expression and Purification of Cyanobacterial Circadian Clock Protein KaiC and Determination of Its Auto-phosphatase Activity
    蓝细菌生物钟蛋白KaiC的表达和纯化及其自磷酸化酶活性测定
    作者:Qiang Chen, Lingling Yu, Xiao Tan and Sen Liu日期:02/20/2017,浏览量:6138,Q&A: 0
    [Abstract] Circadian rhythms are biological processes displaying an endogenous oscillation with a period of ~24 h. They allow organisms to anticipate and get prepared for the environmental changes caused mainly by the rotation of Earth. Circadian rhythms are driven by circadian clocks that consist of proteins, DNA, and/or RNA. Circadian clocks of ...
    Production, Purification and Crystallization of a Prokaryotic SLC26 Homolog for Structural Studies
    生成、纯化和结晶原核生物中SLC26同源蛋白用于结构研究
    作者:Yung-Ning Chang, Farooque R. Shaik, Yvonne Neldner and Eric R. Geertsma日期:02/05/2017,浏览量:6872,Q&A: 0
    [Abstract] The SLC26 or SulP proteins constitute a large family of anion transporters that are ubiquitously expressed in pro- and eukaryotes. In human, SLC26 proteins perform important roles in ion homeostasis and malfunctioning of selected members is associated with diseases. This protocol details the production and crystallization of a prokaryotic SLC26 ...
    Protein Expression Protocol for an Adenylate Cyclase Anchored by a Vibrio Quorum Sensing Receptor
    由弧菌群体感应受体锚定的腺苷酸环化酶膜的催化结构域的蛋白表达方案
    作者:Stephanie Beltz and Joachim E. Schultz日期:01/20/2017,浏览量:6862,Q&A: 0
    [Abstract] The direct regulation of a mycobacterial adenylate cyclase (Rv1625c) via exchange of its membrane anchor by the quorum sensing receptor CqsS (Vibrio harveyi) has recently been reported (Beltz et al., 2016). This protocol describes the expression and membrane preparation for these chimeric proteins.
    Heterologous Expression and Purification of the Magnesium Transporter A (MgtA) in Escherichia coli
    在大肠杆菌中异源表达镁离子转运蛋白(MgtA)并纯化
    作者:Saranya Subramani and Jens Preben Morth日期:11/20/2016,浏览量:7816,Q&A: 0
    [Abstract] The magnesium transporter A (MgtA) is a magnesium transporting P-type ATPase present in prokaryotes and plants (Subramani et al., 2016). In Salmonella typhimurium and Escherichia coli (E. coli), MgtA is expressed only in magnesium limiting conditions and plays an important role in Mg2+ homeostasis ...