哺乳纲

分类

    现刊
    Skin Transplantation and Lymphoid Organ Analysis in Mice
    小鼠中皮肤移植和淋巴器官分析
    作者:Julie Ruer-Laventie, Rajesh Jayachandran, Mathias Schmaler, Simona W. Rossi and Jean Pieters日期:02/20/2020,浏览量:180,Q&A: 0
    [Abstract] Skin transplantation in mice is an important procedure to evaluate immune responses generated against heterologous grafts, especially given its highly immunogenic nature. In fact, skin is one of the most challenging organs in terms of allograft retention. In this protocol, we provide a detailed procedure for skin grafting using the tail skin as ...
    Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands
    小鼠乳腺单个上皮细胞悬液制备
    作者:Heng Sun, Xiaoling Xu and Chuxia Deng日期:02/20/2020,浏览量:74,Q&A: 0
    [Abstract] Single cell RNA sequencing is a very powerful means for cellular heterogeneous studies and so becoming wildly utilized nowadays. To guarantee the success of such analysis, it is very important, though sometimes difficult, to obtain single cells suspension with high quality, especially from the primary solid organs like mammary glands. Digestion of ...
    Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer’s disease
    阿尔茨海默症幼鼠模型中海马单细胞记录和海马依赖先天性行为
    [Abstract] Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task evaluations provides a reliable experimental approach to detect fine changes during early phases of ...
    RNA Sequencing of Single Myofibers from Mus musculus
    小鼠单肌纤维的RNA序列分析
    作者:Darren M. Blackburn, Felicia Lazure and Vahab D. Soleimani日期:02/20/2020,浏览量:319,Q&A: 0
    [Abstract] Whole transcriptome analysis is a key method in biology that allows researchers to determine the effect a condition has on gene regulation. One difficulty in RNA sequencing of muscle is that traditional methods are performed on the whole muscle, but this captures non-myogenic cells that are part of the muscle. In order to analyze only the ...
    Induction of Temporal Lobe Epilepsy in Mice with Pilocarpine
    毛果芸香碱诱导小鼠颞叶癫痫
    作者:Muhammad Nauman Arshad and Janice R Naegele日期:02/20/2020,浏览量:164,Q&A: 0
    [Abstract] In the pilocarpine model of temporal lobe epilepsy (TLE) in rodents, systemic injections of pilocarpine induce continuous, prolonged limbic seizures, a condition termed “Status Epilepticus” (SE). With appropriate doses, many inbred strains of mice show behavioral seizures within an hour after pilocarpine is injected. With the behavioral scoring ...
    Method for Primary Epithelial Cell Culture from the Rat Choroid Plexus
    大鼠脉络丛原代上皮细胞培养方法
    作者:Valeria Lallai, Amina Ahmed and Christie D. Fowler日期:02/20/2020,浏览量:76,Q&A: 0
    [Abstract] The choroid plexus consists of a network of secretory epithelial cells localized throughout the lateral, third and fourth ventricles of the brain. Cerebrospinal fluid (CSF) is generated by the choroid plexus and released into the ventricular environment. This biofluid contains an enriched source of proteins, ions, and other signaling molecules for ...
    Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation
    骨髓和脂肪间充质干细胞:分离、培养、鉴定和分化
    [Abstract] Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and regenerative medicine. In this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and ...
    Transcervical Mouse Infections with Chlamydia trachomatis and Determination of Bacterial Burden
    小鼠宫颈沙眼衣原体感染及细菌负荷测定
    作者:Karthika Rajeeve and Rajeeve Sivadasan日期:02/05/2020,浏览量:232,Q&A: 0
    [Abstract] Chlamydia trachomatis is an obligate human pathogen. It infects the genital tract of humans ascending into the fallopian tube, exacerbated by chronic pelvic pain, pelvic inflammatory disease, and fallopian tube scaring resulting in infertility and other malignancies. The major hurdle in controlling chlamydial spread is that the ...
    Implanting and Recycling Neuropixels Probes for Recordings in Freely Moving Mice
    自由运动小鼠 Neuropixels 探针的植入与回收
    作者:Ashley L. Juavinett, George Bekheet and Anne K. Churchland日期:02/05/2020,浏览量:426,Q&A: 0
    [Abstract] Recording neural activity in unrestricted animals is necessary to unravel the neural basis of ethological behaviors. Recently, Neuropixels probes have made important strides in improving yield and lowering noise, but have limited use cases in freely moving animals. Although there are a number of studies demonstrating the use of these probes in ...
    Rapid Nickel-based Isolation of Extracellular Vesicles from Different Biological Fluids
    从不同的生物体液中进行胞外囊泡的快速镍基分离
    [Abstract] Extracellular vesicles (EVs) are membranous structures that cells massively release in extracellular fluids. EVs are cargo of cellular components such as lipids, proteins, and nucleic acids that can work as a formidable source in liquid biopsy studies searching for disease biomarkers. We recently demonstrated that nickel-based isolation (NBI) is a ...