免疫学

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    现刊
    Quantitation of TLR4 Internalization in Response to LPS in Thioglycollate Elicited Peritoneal Mouse Macrophages by Flow Cytometry
    利用流式细胞术对巯基乙醇酸盐诱导的小鼠腹腔巨噬细胞中LPS-TLR4复合物内化的定量分析
    作者:Katharina Richard, Stefanie N. Vogel and Darren J. Perkins日期:09/20/2019,浏览量:207,Q&A: 0
    Internalization of cell surface Toll-like Receptor 4 (TLR4) is a convenient read-out to measure LPS dependent activation of the TRIF adaptor pathway. We here provide a protocol to quantify the LPS dependent internalization of TLR4 using ...
    Immunohistochemical Staining of CD8α in Diabetic Mouse Kidney
    糖尿病小鼠肾脏CD8α的免疫组化染色
    作者:Ming-Zhi Zhang and Raymond C. Harris日期:09/20/2019,浏览量:197,Q&A: 0
    Immune cell infiltration, particularly cytotoxic CD8α lymphocyte infiltration, plays an important role in development of diabetic nephropathy. Although CD8α infiltration can be evaluated by its production of cytokines, its localization in the kidney ...
    A Refined Protocol for Identifying Citrulline-specific Monoclonal Antibodies from Single Human B Cells from Rheumatoid Arthritis Patient Material
    类风湿性关节炎患者单个B细胞中瓜氨酸特异单克隆抗体的改进鉴定方案
    We describe here a detailed, refined protocol for the generation of citrulline-specific monoclonal antibodies from single human B cells from rheumatoid arthritis (RA) patients. This protocol provides a detailed guide of the procedure starting from ...
    Isolation of Neutrophil Nuclei for Use in NETosis Assays
    分离中性粒细胞核用于 NETosis分析
    作者:Dave Boucher日期:09/05/2019,浏览量:424,Q&A: 0
    Neutrophils are critical immune cells that protect our body against invading pathogens. They generate antibacterial DNA structures called neutrophil extracellular traps (NET). Recently we identified a new mechanism that enables NET formation. We ...
    In vitro Differentiation of Thymic Treg Cell Progenitors to Mature Thymic Treg Cells
    体外胸腺调节性T细胞祖细胞向成熟胸腺调节性T细胞的分化
    作者:David L. Owen and Michael A. Farrar日期:08/20/2019,浏览量:318,Q&A: 0
    Thymic Treg cell differentiation occurs via a two-step process. Step one generates Treg cell progenitors (TregP) and is driven by strong TCR interactions with antigens presented in the thymus. Step two is initiated ...
    Fibroblast Gap-closure Assay-Microscopy-based in vitro Assay Measuring the Migration of Murine Fibroblasts
    基于成纤维细胞间隙封闭显微成像的鼠成纤维细胞的体外迁移分析
    作者:Agnieszka P. Looney and Mallar Bhattacharya日期:08/20/2019,浏览量:519,Q&A: 0
    Pulmonary fibrosis is characterized by pathological scaring of the lung. Similar to other fibrotic diseases, scar formation is driven by excessive extracellular matrix deposition by activated, proliferative, and migratory fibroblasts.

    ...
    Quantitation of TLR4 Internalization in Response to LPS in Thioglycollate Elicited Peritoneal Mouse Macrophages by Flow Cytometry
    利用流式细胞术对巯基乙醇酸盐诱导的小鼠腹腔巨噬细胞中LPS-TLR4复合物内化的定量分析
    作者:Katharina Richard, Stefanie N. Vogel and Darren J. Perkins日期:09/20/2019,浏览量:207,Q&A: 0
    [Abstract] Internalization of cell surface Toll-like Receptor 4 (TLR4) is a convenient read-out to measure LPS dependent activation of the TRIF adaptor pathway. We here provide a protocol to quantify the LPS dependent internalization of TLR4 using thioglycollate-elicited peritoneal macrophages by flow cytometry.
    Immunohistochemical Staining of CD8α in Diabetic Mouse Kidney
    糖尿病小鼠肾脏CD8α的免疫组化染色
    作者:Ming-Zhi Zhang and Raymond C. Harris日期:09/20/2019,浏览量:197,Q&A: 0
    [Abstract] Immune cell infiltration, particularly cytotoxic CD8α lymphocyte infiltration, plays an important role in development of diabetic nephropathy. Although CD8α infiltration can be evaluated by its production of cytokines, its localization in the kidney is of particular importance. The current protocol describes CD8α immunostaining using a Vectastain ...
    A Refined Protocol for Identifying Citrulline-specific Monoclonal Antibodies from Single Human B Cells from Rheumatoid Arthritis Patient Material
    类风湿性关节炎患者单个B细胞中瓜氨酸特异单克隆抗体的改进鉴定方案
    [Abstract] We describe here a detailed, refined protocol for the generation of citrulline-specific monoclonal antibodies from single human B cells from rheumatoid arthritis (RA) patients. This protocol provides a detailed guide of the procedure starting from single B cells of your choice and followed by amplification of the variable region of immunoglobulin ...
    Isolation of Neutrophil Nuclei for Use in NETosis Assays
    分离中性粒细胞核用于 NETosis分析
    作者:Dave Boucher日期:09/05/2019,浏览量:424,Q&A: 0
    [Abstract] Neutrophils are critical immune cells that protect our body against invading pathogens. They generate antibacterial DNA structures called neutrophil extracellular traps (NET). Recently we identified a new mechanism that enables NET formation. We observed that following recognition of lipopolysaccharides, inflammatory caspases cleave Gasdermin D ...
    In vitro Differentiation of Thymic Treg Cell Progenitors to Mature Thymic Treg Cells
    体外胸腺调节性T细胞祖细胞向成熟胸腺调节性T细胞的分化
    作者:David L. Owen and Michael A. Farrar日期:08/20/2019,浏览量:318,Q&A: 0
    [Abstract] Thymic Treg cell differentiation occurs via a two-step process. Step one generates Treg cell progenitors (TregP) and is driven by strong TCR interactions with antigens presented in the thymus. Step two is initiated by activation of STAT5 via IL-2, or IL-15, leading to mature Treg cells capable of ...
    Fibroblast Gap-closure Assay-Microscopy-based in vitro Assay Measuring the Migration of Murine Fibroblasts
    基于成纤维细胞间隙封闭显微成像的鼠成纤维细胞的体外迁移分析
    作者:Agnieszka P. Looney and Mallar Bhattacharya日期:08/20/2019,浏览量:519,Q&A: 0
    [Abstract] Pulmonary fibrosis is characterized by pathological scaring of the lung. Similar to other fibrotic diseases, scar formation is driven by excessive extracellular matrix deposition by activated, proliferative, and migratory fibroblasts.

    Currently, the two most widely used chemotaxis and cell migration assays are the scratch assay and the ...
    Visualizing Hypoxia in a Murine Model of Candida albicans Infection Using in vivo Biofluorencence
    用活体生物荧光法观察小鼠白念珠菌感染模型中的缺氧情况
    作者:José Pedro Lopes and Constantin F. Urban日期:08/05/2019,浏览量:518,Q&A: 0
    [Abstract] Candida albicans is a leading human fungal pathogen that uses several metabolic adaptations to escape immune cells and causes systemic disease. Here, we describe a protocol for measuring one of these adaptations, the ability to thrive in hypoxic niches. Hypoxia was generated after successful subdermal infection with C. albicans ...
    Precision Technique for Splenectomy Limits Mouse Stress Responses for Accurate and Realistic Measurements for Investigating Inflammation and Immunity
    脾切除术限制小鼠应激反应的精准技术用于精确现实检测炎症和免疫
    作者:Shengwen Calvin Li, Anthony D. Rangel and Mustafa H. Kabeer日期:08/05/2019,浏览量:502,Q&A: 0
    [Abstract] Splenectomy in an animal model requires a standardized technique utilizing best practice to avoid variability which can result in adverse impact to the animal resulting in flawed physiologic responses simply due to technique rather than to the studied variables. In the case of the spleen, often investigators are analyzing the animal immune or ...
    Isolation and Long-term Cultivation of Mouse Alveolar Macrophages
    小鼠肺泡巨噬细胞的分离与长期培养
    作者:Clara Jana-Lui Busch, Jérémy Favret, Laufey Geirsdóttir, Kaaweh Molawi and Michael H. Sieweke日期:07/20/2019,浏览量:1170,Q&A: 0
    [Abstract] Alveolar macrophages (AM) are tissue-resident macrophages that colonize the lung around birth and can self-maintain long-term in an adult organism without contribution of monocytes. AM are located in the pulmonary alveoli and can be harvested by washing the lungs using the method of bronchoalveolar lavage (BAL). Here, we compared different ...
    Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri
    庆大霉素保护试验确定福氏志贺菌侵染人TC7肠上皮细胞的胞内寄生数目
    作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:776,Q&A: 0
    [Abstract] Shigella flexneri is an intracellular bacterial pathogen that gains access to the gut epithelium using a specialized Type III Secretion System (T3SS). Various determinants mediating this invasive infection have been experimentally verified using the classical gentamicin protection assay presented here. In this assay epithelial cell lines ...