免疫学

分类

    现刊
    Ex vivo Assessment of Mitochondrial Function in Human Peripheral Blood Mononuclear Cells Using XF Analyzer
    使用XF分析仪体外评估人外周血单个核细胞线粒体功能
    作者:Alica Schöller-Mann, Katja Matt, Barbara Hochecker and Jörg Bergemann日期:04/05/2021,浏览量:160,Q&A: 0
    [Abstract]

    Cellular health and function, as we know today, depend on a large extent on mitochondrial function. The essential function of mitochondria is the energy production, more precisely ATP production, via oxidative phosphorylation. Mitochondrial energy production parameters therefore represent important biomarkers. Studies on human cells have mainly

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    Using the Cecal Ligation and Puncture Model of Sepsis to Induce Rats to Multiple Organ Dysfunction
    采用败血症盲肠结扎穿刺模型诱导大鼠多脏器功能障碍
    [Abstract]

    Sepsis is a dysregulated hyperinflammatory disease caused by infection. Sepsis leads to multiple organ dysfunction syndrome (MODS), which is associated with high rates of mortality. The cecal ligation and puncture (CLP) model has been widely used in animals and has become the gold-standard method of replicating features of sepsis in humans.

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    Brain-localized and Intravenous Microinjections in the Larval Zebrafish to Assess Innate Immune Response
    对斑马鱼幼体进行脑局部和静脉显微注射以评估先天免疫反应
    作者:Alison M. Rojas and Celia E. Shiau日期:04/05/2021,浏览量:350,Q&A: 0
    [Abstract]

    Creating a robust and controlled infection model is imperative for studying the innate immune response. Leveraging the particular strengths of the zebrafish model system, such as optical transparency, ex utero development, and large clutch size, allows for the development of methods that yield consistent and reproducible results. We created a

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    A Potent Vaccine Delivery System
    一个有效的疫苗输送系统
    作者:Guangzu Zhao, Armira Azuar, Istvan Toth and Mariusz Skwarczynski日期:04/05/2021,浏览量:197,Q&A: 0
    [Abstract]

    Most vaccines require co-delivery of an adjuvant in order to generate the desired immune responses. However, many currently available adjuvants are non-biodegradable, have limited efficacy, and/or poor safety profile. Thus, new adjuvants, or self-adjuvanting vaccine delivery systems, are required. Here, we proposed a self-adjuvanting delivery

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    An Image-based Dynamic High-throughput Analysis of Adherent Cell Migration
    一种基于图像的动态高通量分析黏附细胞的迁移
    [Abstract]

    In this protocol, we describe a method to monitor cell migration by live-cell imaging of adherent cells. Scratching assay is a common method to investigate cell migration or wound healing capacity. However, achieving homogenous scratching, finding the optimal time window for end-point analysis and performing an objective image analysis imply, even

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    Quantitative Measurement of Mucolytic Enzymes in Fecal Samples
    粪便粘液溶解酶的定量测定
    作者:Shahanshah Khan and Hasan Zaki日期:03/20/2021,浏览量:753,Q&A: 0
    [Abstract]

    The mucus layer in the gastrointestinal tract covers the apical surface of intestinal epithelial cells, protecting the mucosal tissue from enteric pathogen and commensal microorganisms. The mucus is primarily composed of glycosylated protein called mucins, which are produced by goblet cells, a type of columnar epithelial cells in the intestinal

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    Liposomal Clodronate-mediated Macrophage Depletion in the Zebrafish Model
    在斑马鱼模型中脂质体氯膦酸盐介导的巨噬细胞耗竭
    作者:Linlin Yang, Alison M. Rojas and Celia E. Shiau日期:03/20/2021,浏览量:678,Q&A: 0
    [Abstract]

    The ability to conduct in vivo macrophage-specific depletion remains an effective means to uncover functions of macrophages in a wide range of physiological contexts. Compared to the murine model, zebrafish offer superior imaging capabilities due to their optical transparency starting from a single-cell stage to throughout larval development.

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    Murine Monocyte and Macrophage Culture
    小鼠单核细胞和巨噬细胞培养
    作者:Simone M. Haag and Aditya Murthy日期:03/20/2021,浏览量:1334,Q&A: 0
    [Abstract]

    Myeloid progenitors in the bone marrow generate monocytes, macrophages, granulocytes and most dendritic cells. Even though these innate immune cells are part of the same lineage, each cell type plays a specific and critical role in tissue development, host defense and the generation of adaptive immunity. Protocols have been developed in the past

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    An Imaging Flow Cytometry Method to Measure Citrullination of H4 Histone as a Read-out for Neutrophil Extracellular Traps Formation
    一种成像流式细胞术测定组蛋白H4瓜氨酸化的方法,作为中性粒细胞胞外陷阱形成的信号通路
    作者:Emilia A. Barbu, Venina M. Dominical, Laurel Mendelsohn and Swee Lay Thein日期:02/20/2021,浏览量:862,Q&A: 0
    [Abstract]

    The formation of neutrophil extracellular traps (NETs) is thought to play a critical role in infections and propagating sterile inflammation. Histone citrullination is an essential and early step in NETs formation, detectable prior to the formation of the hallmark extracellular DNA-scaffolded strands. In addition to the classical microscopy

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    Phytophthora infestans (Late blight) Infection Assay in a Detached Leaf of Potato
    在离体叶片中的马铃薯疫霉(晚疫病)感染实验
    作者:Hari S. Karki and Dennis A. Halterman日期:02/20/2021,浏览量:1125,Q&A: 0
    [Abstract]

    Phytophthora infestans is a hemibiotroph oomycete that primarily infects potato and tomato. It infects stems, leaves, and tubers and fruits of potato and tomato. High throughput and reproducible infection assays are prerequisites to find sources of resistance in any crop. In this protocol, we describe a detached leaf assay (DLA) for conducting the

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