免疫学

分类

    现刊
    Flow-cytometric Detection of Low-level Reactive Oxygen Species in Cell Lines and Primary Immune Cells
    细胞系和原代免疫细胞中的低水平活性氧的流式细胞仪检测
    作者:Kevin Bode, Corinna Link, Peter H. Krammer and Heiko Weyd日期:09/05/2020,浏览量:904,Q&A: 0
    Depending on its concentration and cellular origin the production of reactive oxygen species (ROS) in the organism serves a variety of functions. While high concentrations during an oxidative burst are used to fight pathogens, low to moderate ...
    Optogenetic Tuning of Protein-protein Binding in Bilayers Using LOVTRAP
    LOVTRAP对双层膜蛋白质结合的光遗传学调控
    作者:Doug Tischer and Orion D. Weiner日期:09/05/2020,浏览量:660,Q&A: 0
    Modern microscopy methods are powerful tools for studying live cell signaling and biochemical reactions, enabling us to observe when and where these reactions take place from the level of a cell down to single molecules. With microscopy, each cell ...
    Multiplication and Growth Inhibition Activity Assays for the Zoonotic Malaria Parasite, Plasmodium knowlesi
    人畜共患病疟原虫——诺氏疟原虫增殖和生长抑制活性测定
    作者:Franziska Mohring, Thomas A. Rawlinson, Simon J. Draper and Robert W. Moon日期:09/05/2020,浏览量:445,Q&A: 0
    Malaria remains a major cause of morbidity and mortality globally. Clinical symptoms of the disease arise from the growth and multiplication of Plasmodium parasites within the blood of the host. Thus in vitro assays to determine ...
    Flow-cytometric Detection of Low-level Reactive Oxygen Species in Cell Lines and Primary Immune Cells
    细胞系和原代免疫细胞中的低水平活性氧的流式细胞仪检测
    作者:Kevin Bode, Corinna Link, Peter H. Krammer and Heiko Weyd日期:09/05/2020,浏览量:904,Q&A: 0
    [Abstract] Depending on its concentration and cellular origin the production of reactive oxygen species (ROS) in the organism serves a variety of functions. While high concentrations during an oxidative burst are used to fight pathogens, low to moderate amounts of ROS act as signaling molecules important for several physiological processes such as regulation ...
    Optogenetic Tuning of Protein-protein Binding in Bilayers Using LOVTRAP
    LOVTRAP对双层膜蛋白质结合的光遗传学调控
    作者:Doug Tischer and Orion D. Weiner日期:09/05/2020,浏览量:660,Q&A: 0
    [Abstract] Modern microscopy methods are powerful tools for studying live cell signaling and biochemical reactions, enabling us to observe when and where these reactions take place from the level of a cell down to single molecules. With microscopy, each cell or molecule can be observed both before and after a given perturbation, facilitating better inference ...
    Multiplication and Growth Inhibition Activity Assays for the Zoonotic Malaria Parasite, Plasmodium knowlesi
    人畜共患病疟原虫——诺氏疟原虫增殖和生长抑制活性测定
    作者:Franziska Mohring, Thomas A. Rawlinson, Simon J. Draper and Robert W. Moon日期:09/05/2020,浏览量:445,Q&A: 0
    [Abstract] Malaria remains a major cause of morbidity and mortality globally. Clinical symptoms of the disease arise from the growth and multiplication of Plasmodium parasites within the blood of the host. Thus in vitro assays to determine how drug, antibody and genetic perturbations affect the growth rate of Plasmodium parasites ...
    Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-κB Reporter Mice
    LPS诱导NF-κB报告小鼠局部炎症及药物沉积的实时体内显像研究
    作者:Artur Schmidtchen and Manoj Puthia日期:08/20/2020,浏览量:804,Q&A: 0
    [Abstract] Wound, biomaterial, and surgical infections are all characterized by a localized and excessive inflammation, motivating the development of in vivo methods focused on the analysis of local immune events. However, current inflammation models, such as the commonly used in vivo models of endotoxin-induced inflammation are based on ...
    Neutrophil Extracellular Trap Killing Assay of Candida albicans
    白念珠菌中性粒细胞胞外诱杀试验
    作者:Sheng-Yang Wu and Betty A. Wu-Hsieh日期:08/20/2020,浏览量:695,Q&A: 0
    [Abstract] Fungal pathogen Candida albicans is one of the top leading causes of overall healthcare-associated bloodstream infections worldwide. Neutrophil is the major effector cell to clear C. albicans infection. Our study showed that mouse neutrophils utilize two independent mechanisms to kill C. albicans: one is CR3 downstream ...
    Isolation and Quantification of Extracellular DNA from Biofluids
    生物流体中细胞外DNA的分离和定量
    作者:Ľubica Janovičová, Barbora Konečná, Barbora Vlková and Peter Celec日期:08/20/2020,浏览量:619,Q&A: 0
    [Abstract] Extracellular DNA is studied as a diagnostic biomarker, but also as a factor involved in the pathophysiology of several diseases due to its pro-inflammatory properties. Extracellular DNA can be extracted from plasma, urine, saliva or other biofluids using standard DNA isolation procedures and specialized commercial kits. Sample preparation for ...
    Flow Cytometry of CD14, VDR, Cyp27 and Cyp24 and TLR4 in U937 Cells
    流式细胞术检测U937细胞CD14、VDR、Cyp27、Cyp24和TLR4
    [Abstract] Chronic Kidney Disease (CKD) patients present a micro inflammation state due to failure renal function. The calcitriol has been described as an anti-inflammatory factor that might modulates the inflammatory response in CKD patients. However, these patients have deficiency of Calcitriol due to failure renal function. But, synthesis of this vitamin ...
    Metabolomic and Lipidomic Analysis of Bone Marrow Derived Macrophages
    骨髓衍生巨噬细胞的代谢组学和脂质组学分析
    作者:Gretchen L. Seim, Steven V. John and Jing Fan日期:07/20/2020,浏览量:647,Q&A: 0
    [Abstract] Macrophages are highly plastic immune cells that are capable of adopting a wide array of functional phenotypes in response to environmental stimuli. The changes in macrophage function are often supported and regulated by changes in cellular metabolism. Capturing a comprehensive picture of metabolism is vital for understanding the role of metabolic ...
    Intestinal Enteroid Culture for Human Astroviruses
    人类星状病毒的肠样培养
    作者:Irene A. Owusu, Carmen Mirabelli and Abimbola O. Kolawole日期:07/20/2020,浏览量:376,Q&A: 0
    [Abstract] Human astroviruses (HAstV) are non-enveloped, positive-sense single stranded RNA viruses that typically cause gastroenteritis in children, the elderly and among immunocompromised individuals. Some HAstV species have also been implicated in neurological diseases. It is important to study these viruses to understand the pathogenesis and develop ...
    Microscopy-based Methods for Rosetting Assay in Malaria Research
    基于显微技术的疟疾玫瑰花环试验
    作者:Wenn-Chyau Lee and Laurent Rénia日期:07/05/2020,浏览量:882,Q&A: 0
    [Abstract] In malaria, rosetting phenomenon is a condition where a Plasmodium-infected erythrocyte stably adheres to at least an uninfected erythrocyte. This phenomenon that occurs in all species of human malaria parasite is likely to be an immune escape mechanism for the parasite. However, it has been associated with malaria pathogenesis, possibly ...