免疫学

分类

    现刊
    Skin Transplantation and Lymphoid Organ Analysis in Mice
    小鼠中皮肤移植和淋巴器官分析
    作者:Julie Ruer-Laventie, Rajesh Jayachandran, Mathias Schmaler, Simona W. Rossi and Jean Pieters日期:02/20/2020,浏览量:165,Q&A: 0
    Skin transplantation in mice is an important procedure to evaluate immune responses generated against heterologous grafts, especially given its highly immunogenic nature. In fact, skin is one of the most challenging organs in terms of allograft ...
    Skin Transplantation and Lymphoid Organ Analysis in Mice
    小鼠中皮肤移植和淋巴器官分析
    作者:Julie Ruer-Laventie, Rajesh Jayachandran, Mathias Schmaler, Simona W. Rossi and Jean Pieters日期:02/20/2020,浏览量:165,Q&A: 0
    [Abstract] Skin transplantation in mice is an important procedure to evaluate immune responses generated against heterologous grafts, especially given its highly immunogenic nature. In fact, skin is one of the most challenging organs in terms of allograft retention. In this protocol, we provide a detailed procedure for skin grafting using the tail skin as ...
    Transcervical Mouse Infections with Chlamydia trachomatis and Determination of Bacterial Burden
    小鼠宫颈沙眼衣原体感染及细菌负荷测定
    作者:Karthika Rajeeve and Rajeeve Sivadasan日期:02/05/2020,浏览量:226,Q&A: 0
    [Abstract] Chlamydia trachomatis is an obligate human pathogen. It infects the genital tract of humans ascending into the fallopian tube, exacerbated by chronic pelvic pain, pelvic inflammatory disease, and fallopian tube scaring resulting in infertility and other malignancies. The major hurdle in controlling chlamydial spread is that the ...
    Automated Analysis of Cell Surface Ruffling: Ruffle Quantification Macro
    细胞表面Ruffling的自动分析:Ruffle的宏观量化
    作者:Nicholas D. Condon, Jennifer L. Stow and Adam A. Wall日期:01/20/2020,浏览量:303,Q&A: 0
    [Abstract] Cell surface protrusions include F-actin rich, wave-like ruffles that are erected transiently in response to stimuli and during cell migration. Macrophages are innate immune cells that ruffle constitutively and more dramatically in cells activated by pathogens. Dorsal ruffles and their resulting macropinosomes are key sites for environmental ...
    Quantifying HIV-1-Mediated Gut CD4+ T Cell Death in the Lamina Propria Aggregate Culture (LPAC) Model
    固有层聚集培养模式中HIV-1介导的Gut CD4+ T细胞凋亡的量化分析
    作者:Stephanie M. Dillon, Kejun Guo, Moriah J. Castleman, Mario L. Santiago and Cara C. Wilson日期:01/20/2020,浏览量:327,Q&A: 0
    [Abstract] Gut CD4 T cells are major targets of HIV-1 and are massively depleted early during infection. To better understand the mechanisms governing HIV-1-mediated CD4 T cell death, we developed the physiologically-relevant Lamina Propria Aggregate Culture (LPAC) model. The LPAC model is ideal for studying CD4 T cell death induced by clinically-relevant ...
    Isolation and Stimulation of Peritoneal Macrophages with Apoptotic Jurkat Cells to Produce IL-10
    提取和利用凋亡Jurkat细胞诱导腹腔巨噬细胞分泌白介素-10
    作者:Mei Song and Xiaojing Ma日期:12/20/2019,浏览量:486,Q&A: 0
    [Abstract] Clearance of apoptotic cells by macrophages is critical to ensuring cellular homeostasis and suppression of autoimmunity. Macrophage recognition of apoptotic cells triggers an anti-inflammatory response, which is mediated by the release of IL-10, TGF-β etc. with concurrent inhibition of pro-inflammatory cytokines (such as TNFα, IL-12, ...
    Propagation and Purification of Chlamydia trachomatis Serovar L2 Transformants and Mutants
    沙眼衣原体血清型L2转化子和突变体的繁殖及纯化
    作者:Robert Faris and Mary M. Weber日期:12/20/2019,浏览量:464,Q&A: 0
    [Abstract] Chlamydia trachomatis (C.t.) is an obligate intracellular pathogen that cannot be cultured axenically and must be propagated within eukaryotic host cells. There are at least 15 distinct chlamydial serovariants that belong to 2 major biovars commonly referred to as trachoma and lymphogranuloma venereum (LGV). The invasive ...
    Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells
    溶酶体靶向红绿试验
    作者:Victoria Riccio, Miluska Vissa, Angus McQuibban and Peter Kijun Kim日期:12/20/2019,浏览量:799,Q&A: 0
    [Abstract] The process of autophagy is an essential cellular mechanism, required to maintain general cell health through the removal of dysfunctional organelles, such as the ER, peroxisomes and mitochondria, as well as protein aggregates, and bacteria. Autophagy is an extremely dynamic process, and tools are constantly being developed to study the various ...
    Preparation of Red Palm Weevil Rhynchophorus Ferrugineus (Olivier) (Coleoptera: Dryophthoridae) Germ-free Larvae for Host-gut Microbes Interaction Studies
    用于宿主肠道微生物相互作用研究的红棕象甲(稻铁甲虫)(鞘翅目:椰象鼻虫科)无菌幼虫的制备
    作者:Abrar Muhammad, Prosper Habineza, Youming Hou and Zhanghong Shi日期:12/20/2019,浏览量:518,Q&A: 0
    [Abstract] Red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, is a devastating pest of palm trees worldwide. RPW gut is colonized by diverse bacterial species which profoundly influence host development and nutritional metabolism. However, the molecular mechanisms behind the interactions between RPW and its gut microbiota remain mostly ...
    cDNA Display Mediated Immuno-PCR (cD-IPCR): A Novel PCR-based Antigen Detection Method
    cDNA免疫PCR:一种新的基于PCR的抗原检测方法
    作者:Chathuni Jayathilake, Takuya Terai and Naoto Nemoto日期:12/20/2019,浏览量:712,Q&A: 0
    [Abstract] Immuno-PCR (IPCR) is a powerful method in antigen detection where a PCR-amplifiable DNA reporter is conjugated to a specific antibody or an aptamer for the target molecule. In the development and application of IPCR, successful conjugation of a protein (an antibody) with a reporter DNA becomes challenging. To address this issue, we recently ...
    Quantification of Nitric Oxide and Reactive Oxygen Species in Leishmania-infected J774A.1 Macrophages as a Response to the in vitro Treatment with a Natural Product Compound
    感染利什曼原虫J774A.1的巨噬细胞响应体外天然化合物处理的一氧化氮和活性氧定量分析
    作者:Kalliopi Karampetsou, Olga S. Koutsoni and Eleni Dotsika日期:12/05/2019,浏览量:493,Q&A: 0
    [Abstract] Leishmaniasis is a parasitic disease caused by the obligatory intracellular protozoa Leishmania spp. Current therapeutic options are limited and thus, drug discovery against leishmaniasis is very important. Nevertheless, there is a great difficulty to develop therapeutic strategies against the disease because the parasite deploys various ...