发育生物学

分类

    现刊
    En masse DNA Electroporation for in vivo Transcriptional Assay in Ascidian Embryos
    海鞘胚胎中DNA电穿孔的体内转录分析
    作者:Sébastien Darras日期:09/20/2021,浏览量:528,Q&A: 0

    Ascidian embryos are powerful models for functional genomics, in particular, due to the ease of generating a large number of transgenic embryos by electroporation. In addition, the small size of their genome makes them an attractive model for

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    Inter-species Transplantation of Blastocysts between Medaka and Zebrafish
    青鳉与斑马鱼间囊胚的种间移植
    作者:Jana F. Fuhrmann, Jasmin Onistschenko and Lázaro Centanin日期:09/20/2021,浏览量:461,Q&A: 0

    Transplantation of blastocysts from a donor to a host blastula constitutes a powerful experimental tool to tackle major developmental biology questions. The technique is widely implemented in diverse biological models including teleost fish, where

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    Quantitative Analysis of RNA Editing at Specific Sites in Plant Mitochondria or Chloroplasts Using DNA Sequencing
    利用DNA测序对植物线粒体或叶绿体特定位点RNA编辑的定量分析
    作者:Yang Yang and Weixing Shan日期:09/20/2021,浏览量:265,Q&A: 0

    Cytidine-to-uridine (C-to-U) RNA editing is one of the most important post-transcriptional RNA processing in plant mitochondria and chloroplasts. Several techniques have been developed to detect the RNA editing efficiency in plant mitochondria and

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    En masse DNA Electroporation for in vivo Transcriptional Assay in Ascidian Embryos
    海鞘胚胎中DNA电穿孔的体内转录分析
    作者:Sébastien Darras日期:09/20/2021,浏览量:528,Q&A: 0
    [Abstract]

    Ascidian embryos are powerful models for functional genomics, in particular, due to the ease of generating a large number of transgenic embryos by electroporation. In addition, the small size of their genome makes them an attractive model for studying cis-regulatory elements that control gene expression during embryonic development. Here, I

    ...
    Inter-species Transplantation of Blastocysts between Medaka and Zebrafish
    青鳉与斑马鱼间囊胚的种间移植
    作者:Jana F. Fuhrmann, Jasmin Onistschenko and Lázaro Centanin日期:09/20/2021,浏览量:461,Q&A: 0
    [Abstract]

    Transplantation of blastocysts from a donor to a host blastula constitutes a powerful experimental tool to tackle major developmental biology questions. The technique is widely implemented in diverse biological models including teleost fish, where it is typically used for intra-species blastula transplantations – i.e., labeled blastocysts

    ...
    Quantitative Analysis of RNA Editing at Specific Sites in Plant Mitochondria or Chloroplasts Using DNA Sequencing
    利用DNA测序对植物线粒体或叶绿体特定位点RNA编辑的定量分析
    作者:Yang Yang and Weixing Shan日期:09/20/2021,浏览量:265,Q&A: 0
    [Abstract]

    Cytidine-to-uridine (C-to-U) RNA editing is one of the most important post-transcriptional RNA processing in plant mitochondria and chloroplasts. Several techniques have been developed to detect the RNA editing efficiency in plant mitochondria and chloroplasts, such as poisoned primer extension (PPE) assays, high-resolution melting (HRM) analysis,

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    In situ Hybridization of miRNAs in Human Embryonic Kidney and Human Pluripotent Stem Cell-derived Kidney Organoids
    在人胚肾和人多能干细胞衍生的肾类器官中微小RNA的原位杂交
    作者:Filipa M. Lopes, Susan J. Kimber and Ioannis Bantounas日期:09/05/2021,浏览量:421,Q&A: 0
    [Abstract]

    MicroRNAs are small RNAs that negatively regulate gene expression and play an important role in fine-tuning molecular pathways during development. There is increasing interest in studying their function in the kidney, but the majority of studies to date use kidney cell lines and assess the total amounts of miRNAs of interest either by qPCR or by

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    Isolation of Myofibres and Culture of Muscle Stem Cells from Adult Zebrafish
    成年斑马鱼肌纤维的分离和肌肉干细胞的培养
    作者:Massimo Ganassi, Peter S. Zammit and Simon M. Hughes日期:09/05/2021,浏览量:1255,Q&A: 0
    [Abstract]

    Skeletal muscles generate force throughout life and require maintenance and repair to ensure efficiency. The population of resident muscle stem cells (MuSCs), termed satellite cells, dwells beneath the basal lamina of adult myofibres and contributes to both muscle growth and regeneration. Upon exposure to activating signals, MuSCs proliferate to

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    Direct-TRI: High-throughput RNA-extracting Method for all Stages of Zebrafish Development
    Direct-TRI: 斑马鱼发育各阶段高通量RNA提取方法
    作者:Kota Ujibe, Kanako Nishimura, Makoto Kashima and Hiromi Hirata日期:09/05/2021,浏览量:483,Q&A: 0
    [Abstract]

    Recent popularization of next-generation sequencing enables conducting easy transcriptome analysis. Nevertheless, substantial RNA isolation work prior to RNA sequencing, as well as the high cost involved, still makes the routine use of large-scale transcriptome analysis difficult. For example, conventional phenol-chloroform RNA extraction cannot

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    Preparation of Drosophila Larval Blood Cells for Single-cell RNA Sequencing
    果蝇幼虫血细胞单细胞RNA测序的制备
    作者:Sudhir Gopal Tattikota and Norbert Perrimon日期:08/20/2021,浏览量:546,Q&A: 0
    [Abstract]

    Recent advances in single-cell RNA-sequencing (scRNA-seq) technologies provide unprecedented opportunities to identify new cell types and characterize cell states. One of the most important requirements for performing scRNA-seq is to obtain high-quality single cells in suspension. Recently, we used this approach to characterize Drosophila blood

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    Measuring DNA Damage Using the Alkaline Comet Assay in Cultured Cells
    培养细胞DNA损伤的碱性彗星试验
    作者:Elena Clementi, Zuzana Garajova and Enni Markkanen日期:08/20/2021,浏览量:983,Q&A: 0
    [Abstract]

    Maintenance of DNA integrity is of pivotal importance for cells to circumvent detrimental processes that can ultimately lead to the development of various diseases. In the face of a plethora of endogenous and exogenous DNA-damaging agents, cells have evolved a variety of DNA repair mechanisms that are responsible for safeguarding genetic

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    A Genetically Engineered Mouse Model of Venous Anomaly and Retinal Angioma-like Vascular Malformation
    静脉畸形和视网膜血管瘤样血管畸形的基因工程小鼠模型
    作者:Xudong Cao, Beibei Xu, Xiao Li, Taotao Li and Yulong He日期:08/05/2021,浏览量:795,Q&A: 0
    [Abstract]

    Characterization of key regulators in vein development will advance our understanding of mechanisms underlying venous anomalies and provide therapeutic targets for the treatment of vascular malformations. Here, we provide a detailed protocol for the generation of genetically engineered mouse models targeting the Tek gene for the analysis of vein

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    Fe-NTA Microcolumn Purification of Phosphopeptides from Immunoprecipitation (IP) Eluates for Mass Spectrometry Analysis
    Fe-NTA微柱纯化免疫沉淀(IP)洗脱物中磷酸肽的质谱分析
    作者:Ethan J. Sanford and Marcus B. Smolka日期:08/05/2021,浏览量:535,Q&A: 0
    [Abstract]

    Protein phosphorylation is a nearly universal signaling mechanism. To date, a number of proteomics tools have been developed to analyze phosphorylation. Phosphoproteome-wide analyses using whole cell extracts suffer from incomplete coverage, often missing phosphorylation events from low-abundance proteins. In order to increase coverage of

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