发育生物学

分类

    现刊
    Fluorescent Polysome Profiling in Caenorhabditis elegans
    秀丽隐杆线虫荧光多聚体分析
    作者:Dan Shaffer and Jarod A Rollins日期:09/05/2020,浏览量:545,Q&A: 0
    An important but often overlooked aspect of gene regulation occurs at the level of protein translation. Many genes are regulated not only by transcription but by their propensity to be recruited to actively translating ribosomes (polysomes). ...
    Fluorescent Polysome Profiling in Caenorhabditis elegans
    秀丽隐杆线虫荧光多聚体分析
    作者:Dan Shaffer and Jarod A Rollins日期:09/05/2020,浏览量:545,Q&A: 0
    [Abstract] An important but often overlooked aspect of gene regulation occurs at the level of protein translation. Many genes are regulated not only by transcription but by their propensity to be recruited to actively translating ribosomes (polysomes). Polysome profiling allows for the separation of unbound 40S and 60S subunits, 80S monosomes, and actively ...
    A Reproducible Protocol to Measure the Critical Swimming Speed of Adult Zebrafish
    一种可重复性测定成年斑马鱼临界游泳速度的方法
    作者:Yuma Wakamatsu, Makoto Kashima and Hiromi Hirata日期:08/20/2020,浏览量:563,Q&A: 0
    [Abstract] The quantitative measurement of water flow-induced swimming of fish species using a swimmill is a powerful method to evaluate motor ability of individual fish. Zebrafish is a commonly used vertebrate that enables the study of morphological, physiological and behavioral characteristics associated with genes. We here established a reproducible ...
    Mechanical Tissue Compression and Whole-mount Imaging at Single Cell Resolution for Developing Murine Epididymal Tubules
    小鼠附睾小管发育过程中的组织机械压缩和单细胞分辨率全胚胎成像
    作者:Tsuyoshi Hirashima日期:08/05/2020,浏览量:724,Q&A: 0
    [Abstract] Cells inside the body are subjected to various mechanical stress, such as stretch or compression provided by surrounding cells, shear stresses by blood or lymph flows, and normal stresses by luminal liquids. Force loading to the biological tissues is a fundamental method to better understand cellular responses to such mechanical stimuli. There ...
    Isolation, Culture, and Differentiation of Primary Myoblasts Derived from Muscle Satellite Cells
    肌卫星细胞原代成肌细胞的分离、培养和分化
    作者:Kun Ho Kim, Jiamin Qiu and Shihuan Kuang日期:07/20/2020,浏览量:467,Q&A: 0
    [Abstract] The skeletal muscle is key for body mobility and motor performance, but aging and diseases often lead to progressive loss of muscle mass due to wasting or degeneration of muscle cells. Muscle satellite cells (MuSCs) represent a population of tissue stem cells residing in the skeletal muscles and are responsible for homeostatic maintenance and ...
    In vitro Induction and Detection of Acrosomal Exocytosis in Human Spermatozoa
    人精子中顶体胞吐作用的体外诱导和检测
    作者:Shenae L. Cafe, Amanda L. Anderson and Brett Nixon日期:07/20/2020,浏览量:380,Q&A: 0
    [Abstract] The acrosome reaction is a highly regulated exocytotic event that primes spermatozoa for successful fertilization. Upon induction, acrosomal exocytosis proceeds via a wave of vesiculation that radiates across the sperm head, destabilizing the acrosomal vesicle and resulting in the release of the acrosomal contents. Having shed their acrosome, ...
    Organotypic Slice Culture of the Embryonic Mouse Brain
    胚胎小鼠大脑的器官型切片培养
    作者:James M. Clegg and Thomas Pratt日期:07/05/2020,浏览量:560,Q&A: 0
    [Abstract] Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.
    Primed Track: Reliable Volumetric Single-cell Tracking and Lineage Tracing of Living Specimen with Dual-labeling Approaches
    引物轨迹:可靠的单细胞体积追踪和双标记活标本的谱系追踪
    作者:Maaike Welling, Konstantinos Kalyviotis and Periklis Pantazis日期:06/05/2020,浏览量:841,Q&A: 0
    [Abstract] Mammalian embryonic development starts with a single fertilized zygote that develops into a blastocyst embryo consisting of three cell types that evolve into either embryonic or extra-embryonic tissues. Lineage tracing of these cells can provide important information about the molecular and cellular dynamics contributing to fate allocation during ...
    Ex vivo Culture Assay Using Human Hair Follicles to Study Circadian Characteristics
    通过人类毛囊体外培养研究昼夜节律特征
    作者:Atsuhiro Nishida, Yoshiki Miyawaki, Koichi Node and Makoto Akashi日期:06/05/2020,浏览量:756,Q&A: 0
    [Abstract] Ex vivo culture assays of biopsy specimens are advantageous for the experimental evaluation of human circadian characteristics. We developed a simple and non-invasive experimental evaluation method for monitoring the expression of circadian clock genes in an ex vivo culture assay using human hair follicles. This method imposes ...
    FRET Reporter Assays for cAMP and Calcium in a 96-well Format Using Genetically Encoded Biosensors Expressed in Living Cells
    利用活细胞表达的基因编码生物传感器对96孔甲酸钙和cAMP的FRET检测
    作者:Brandon T. Milliken, Robert P. Doyle, George G. Holz and Oleg G. Chepurny日期:06/05/2020,浏览量:1170,Q&A: 0
    [Abstract] Stimulation of G protein-coupled receptors (GPCR) by hormones and neurotransmitters elicits cellular responses, many of which result from alterations in the concentrations of cytosolic cAMP and Ca2+. Here, we describe a microplate reader fluorescence resonance energy transfer (FRET) assay that uses the genetically encoded biosensors ...
    Direct Reprogramming of Mouse Embryonic Fibroblasts to Conventional Type 1 Dendritic Cells by Enforced Expression of Transcription Factors
    转录因子强化表达直接将小鼠胚胎成纤维细胞重编程为常规1型树突状细胞
    作者:Fábio F. Rosa, Cristiana F. Pires, Olga Zimmermannova and Carlos-Filipe Pereira日期:05/20/2020,浏览量:1281,Q&A: 0
    [Abstract] Ectopic expression of transcription factor combinations has been recently demonstrated to reprogram differentiated somatic cells towards the dendritic cell (DC) lineage without reversion to a multipotent state. DCs have the ability to induce potent and long-lasting adaptive immune responses. In particular, conventional type 1 DCs (cDC1s) excel on ...