发育生物学

分类

    现刊
    Production of Phenotypically Uniform Human Cerebral Organoids from Pluripotent Stem Cells
    从多能干细胞生产表型一致的人类大脑类器官
    作者:Adam Sivitilli, Parisa Ghiasi and Liliana Attisano日期:04/20/2021,浏览量:22,Q&A: 0

    Recent advances in stem cell technology have allowed researchers to generate 3D cerebral organoids (COs) from human pluripotent stem cells (hPSCs). Indeed, COs have provided an unprecedented opportunity to model the developing human brain in a 3D

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    Development of a Chemical Reproductive Aging Model in Female Rats
    雌性大鼠化学生殖衰老模型的建立

    Women are born with an abundant but finite pool of ovarian follicles, which naturally and progressively decreased during their reproductive years until menstrual periods stop permanently (menopause). Perimenopause represents the transition from

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    Development of a Chemical Reproductive Aging Model in Female Rats
    雌性大鼠化学生殖衰老模型的建立
    [Abstract]

    Women are born with an abundant but finite pool of ovarian follicles, which naturally and progressively decreased during their reproductive years until menstrual periods stop permanently (menopause). Perimenopause represents the transition from reproductive to non-reproductive life. It is usually characterized by neuroendocrine, metabolic and

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    Production of Phenotypically Uniform Human Cerebral Organoids from Pluripotent Stem Cells
    从多能干细胞生产表型一致的人类大脑类器官
    作者:Adam Sivitilli, Parisa Ghiasi and Liliana Attisano日期:04/20/2021,浏览量:22,Q&A: 0
    [Abstract]

    Recent advances in stem cell technology have allowed researchers to generate 3D cerebral organoids (COs) from human pluripotent stem cells (hPSCs). Indeed, COs have provided an unprecedented opportunity to model the developing human brain in a 3D context, and in turn, are suitable for addressing complex neurological questions by leveraging

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    A Workflow for High-pressure Freezing and Freeze Substitution of the Caenorhabditis elegans Embryo for Ultrastructural Analysis by Conventional and Volume Electron Microscopy
    用常规和体积电子显微镜对秀丽隐杆线虫胚胎进行超微结构分析的高压冷冻和冷冻替代的工作流程
    作者:Mohammad M. Rahman, Irene Y. Chang, Orna Cohen-Fix and Kedar Narayan日期:04/05/2021,浏览量:226,Q&A: 0
    [Abstract]

    The free-living nematode Caenorhabditis elegans is a popular model system for studying developmental biology. Here we describe a detailed protocol to high-pressure freeze the C. elegans embryo (either ex vivo after dissection, or within the intact worm) followed by quick freeze substitution. Processed samples are suitable for ultrastructural

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    Analysis of TORC1-body Formation in Budding Yeast
    芽殖酵母TORC1-body形成的分析
    作者:Ryan L. Wallace, Eric Lu, Arron Sullivan, James E. Hughes Hallett and Andrew P. Capaldi日期:04/05/2021,浏览量:192,Q&A: 0
    [Abstract]

    The Target of Rapamycin kinase Complex I (TORC1) is the master regulator of cell growth and metabolism in eukaryotes. In the presence of pro-growth hormones and abundant nutrients, TORC1 is active and drives protein, lipid, and nucleotide synthesis by phosphorylating a wide range of proteins. In contrast, when nitrogen and/or glucose levels fall,

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    Ligand and Carbohydrate Engagement (LACE) Assay and Fluorescence Quantification on Murine Neural Tissue
    小鼠神经组织中配体和碳水化合物结合(LACE)实验和荧光定量测定
    作者:James M. Clegg and Thomas Pratt日期:03/20/2021,浏览量:425,Q&A: 0
    [Abstract]

    The interaction between cell surface heparan sulphate and diffusible ligands such as FGFs is of vital importance for downstream signaling, however, there are few techniques that can be used to investigate this binding event. The ligand and carbohydrate engagement (LACE) assay is a powerful tool which can be used to probe the molecular interaction

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    Live Intravital Intestine with Blood Flow Visualization in Neonatal Mice Using Two-photon Laser Scanning Microscopy
    用双光子激光扫描显微镜观察新生小鼠活体肠内血流
    [Abstract]

    This protocol describes a novel technique to investigate the microcirculation dynamics underlying the pathology in the small intestine of neonatal mice using two-photon laser-scanning microscopy (TPLSM). Recent technological advances in multi-photon microscopy allow intravital analysis of different organs such as the liver, brain and intestine.

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    Automated Analysis of Cerebrospinal Fluid Flow and Motile Cilia Properties in The Central Canal of Zebrafish Embryos
    斑马鱼胚胎中央管中脑脊液流动和运动纤毛特性的自动分析
    作者:Olivier Thouvenin, Yasmine Cantaut-Belarif, Ludovic Keiser, François Gallaire and Claire Wyart日期:03/05/2021,浏览量:773,Q&A: 0
    [Abstract]

    Circulation of cerebrospinal fluid (CSF) plays an important role during development. In zebrafish embryo, the flow of CSF has been found to be bidirectional in the central canal of the spinal cord. In order to compare conditions and genetic mutants across each other, we recently automated the quantification of the velocity profile of exogenous

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    Molecular and Phenotypic Characterization Following RNAi Mediated Knockdown in Drosophila
    RNAi介导的基因敲除后果蝇的分子和表型特征
    作者:Saurabh Jayesh Kumar Mehta, Pradyumna A. Joshi and Ram Kumar Mishra日期:02/20/2021,浏览量:921,Q&A: 0
    [Abstract]

    Loss of function studies shed significant light on the involvement of a gene or gene product in different cellular processes. Short hairpin RNA (shRNA) mediated RNA interference (RNAi) is a classical yet straightforward technique frequently used to knock down a gene for assessing its function. Similar perturbations in gene expression can be

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    Extraction and Quantification of Sphingolipids from Hemiptera Insects by Ultra-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry
    超高效液相色谱-串联质谱联用法提取和定量半翅目昆虫鞘脂质
    作者:Ni Wang, Xiaoxiao Shi, Chao Zhang, Wenwu Zhou and Zeng-Rong Zhu日期:02/20/2021,浏览量:811,Q&A: 0
    [Abstract]

    Sphingolipids are major structural components of endomembranes and have also been described as an intracellular second messenger involved in various biological functions in all eukaryotes and a few prokaryotes. Ceramides (Cer), the central molecules of sphingolipids, have been depicted in cell growth arrest, cell differentiation, and apoptosis.

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    Ecdysone Quantification from Whole Body Samples of Drosophila melanogaster Larvae
    黑腹果蝇幼虫全身蜕皮激素的定量分析
    作者:Takashi Koyama and Christen K. Mirth日期:02/05/2021,浏览量:484,Q&A: 0
    [Abstract]

    Steroid hormones strictly control the timing of sexual maturation and final body size both in vertebrates and invertebrates. In insects, the steroid hormone ecdysone controls the timing of the molts between larval instars as well as the transition to metamorphosis. Growth during the final instar accounts for over 80% of the increase in final mass

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