发育生物学

分类

    现刊
    Organotypic Slice Culture of the Embryonic Mouse Brain
    胚胎小鼠大脑的器官型切片培养
    作者:James M. Clegg and Thomas Pratt日期:07/05/2020,浏览量:0,Q&A: 0
    Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays ...
    Organotypic Slice Culture of the Embryonic Mouse Brain
    胚胎小鼠大脑的器官型切片培养
    作者:James M. Clegg and Thomas Pratt日期:07/05/2020,浏览量:0,Q&A: 0
    [Abstract] Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.
    Primed Track: Reliable Volumetric Single-cell Tracking and Lineage Tracing of Living Specimen with Dual-labeling Approaches
    引物轨迹:可靠的单细胞体积追踪和双标记活标本的谱系追踪
    作者:Maaike Welling, Konstantinos Kalyviotis and Periklis Pantazis日期:06/05/2020,浏览量:549,Q&A: 0
    [Abstract] Mammalian embryonic development starts with a single fertilized zygote that develops into a blastocyst embryo consisting of three cell types that evolve into either embryonic or extra-embryonic tissues. Lineage tracing of these cells can provide important information about the molecular and cellular dynamics contributing to fate allocation during ...
    Ex vivo Culture Assay Using Human Hair Follicles to Study Circadian Characteristics
    通过人类毛囊体外培养研究昼夜节律特征
    作者:Atsuhiro Nishida, Yoshiki Miyawaki, Koichi Node and Makoto Akashi日期:06/05/2020,浏览量:469,Q&A: 0
    [Abstract] Ex vivo culture assays of biopsy specimens are advantageous for the experimental evaluation of human circadian characteristics. We developed a simple and non-invasive experimental evaluation method for monitoring the expression of circadian clock genes in an ex vivo culture assay using human hair follicles. This method imposes ...
    FRET Reporter Assays for cAMP and Calcium in a 96-well Format Using Genetically Encoded Biosensors Expressed in Living Cells
    利用活细胞表达的基因编码生物传感器对96孔甲酸钙和cAMP的FRET检测
    作者:Brandon T. Milliken, Robert P. Doyle, George G. Holz and Oleg G. Chepurny日期:06/05/2020,浏览量:642,Q&A: 0
    [Abstract] Stimulation of G protein-coupled receptors (GPCR) by hormones and neurotransmitters elicits cellular responses, many of which result from alterations in the concentrations of cytosolic cAMP and Ca2+. Here, we describe a microplate reader fluorescence resonance energy transfer (FRET) assay that uses the genetically encoded biosensors ...
    Direct Reprogramming of Mouse Embryonic Fibroblasts to Conventional Type 1 Dendritic Cells by Enforced Expression of Transcription Factors
    转录因子强化表达直接将小鼠胚胎成纤维细胞重编程为常规1型树突状细胞
    作者:Fábio F. Rosa, Cristiana F. Pires, Olga Zimmermannova and Carlos-Filipe Pereira日期:05/20/2020,浏览量:819,Q&A: 0
    [Abstract] Ectopic expression of transcription factor combinations has been recently demonstrated to reprogram differentiated somatic cells towards the dendritic cell (DC) lineage without reversion to a multipotent state. DCs have the ability to induce potent and long-lasting adaptive immune responses. In particular, conventional type 1 DCs (cDC1s) excel on ...
    Application of Mechanical Forces on Drosophila Embryos by Manipulation of Microinjected Magnetic Particles
    机械力应用于果蝇胚胎的磁性粒子显微注射操作
    作者:Arturo D’Angelo and Jérôme Solon日期:05/05/2020,浏览量:589,Q&A: 0
    [Abstract] Cells generate mechanical forces to shape tissues during morphogenesis. These forces can activate several biochemical pathways and trigger diverse cellular responses by mechano-sensation, such as differentiation, division, migration and apoptosis. Assessing the mechano-responses of cells in living organisms requires tools to apply controlled local ...
    Live Cell Imaging of Male Meiosis in Arabidopsis by a Landmark-based System
    地标系统在拟南芥雄性减数分裂活体细胞成像中的应用
    作者:Maria Ada Prusicki, Emma Mathilde Keizer, Rik Peter van Rosmalen, Christian Fleck and Arp Schnittger日期:05/05/2020,浏览量:1012,Q&A: 0
    [Abstract] Live cell imaging has tremendously promoted our understanding of cellular and subcellular processes such as cell division. Here, we present a step-by-step protocol for a robust and easy-to-use live cell imaging approach to study male meiosis in the plant Arabidopsis thaliana as recently established. Our method relies on the concomitant ...
    A Modified Semisolid Clonal Culture for Identification of B-1 and B-2 Progenitor Colony Forming Ability of Mouse Embryonic Hemogenic Endothelial Cells
    改良半固态克隆培养鉴定小鼠胚胎造血内皮细胞B-1和B-2祖细胞集落形成能力
    作者:Michihiro Kobayashi and Momoko Yoshimoto日期:05/05/2020,浏览量:659,Q&A: 0
    [Abstract] The search for the origin of the first hematopoietic stem cells (HSCs) in the mouse embryo has been a hot topic in the field of developmental hematopoiesis. Detecting lymphoid potential is one of the supportive evidence to show the definitive hematopoietic activity of HSCs. However, the first B-lymphoid potential in the mouse embryos are reported ...
    Auxin-mediated Protein Degradation in Caenorhabditis elegans
    生长素介导的秀丽隐杆线虫蛋白质降解
    作者:Michael A. Q. Martinez and David Q. Matus日期:04/20/2020,浏览量:927,Q&A: 0
    [Abstract] The auxin-inducible degron (AID) technology was recently adapted for use in the nematode Caenorhabditis elegans. Rapid degradation of C. elegans proteins tagged with an AID is mediated by a plant-specific F-box protein, transport inhibitor response 1 (TIR1), and occurs only in the presence of the phytohormone auxin. The first ...
    Bimolecular Fluorescence Complementation (BiFC) for Studying Sarcomeric Protein Interactions in Drosophila
    双分子荧光互补法研究果蝇肌节蛋白相互作用
    作者:Océane Marescal, Frieder Schöck and Nicanor González-Morales日期:04/05/2020,浏览量:544,Q&A: 0
    [Abstract] Protein-protein interactions in Drosophila myofibrils are essential for their function and formation. Bimolecular Fluorescence Complementation (BiFC) is an effective method for studying protein interactions and localization. BiFC relies on the reconstitution of a monomeric fluorescent protein from two half-fragments when in proximity. Two ...