发育生物学

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    现刊
    Application of a Modified Smart-seq2 Sample Preparation Protocol for Rare Cell Full-length Single-cell mRNA Sequencing to Mouse Oocytes
    一种改良的Smart-seq2样品制备方案在小鼠卵母细胞中应用稀有细胞全长单细胞mRNA测序
    作者:Rebecca S. Treger, Scott D. Pope, Xiaojun Xing and Akiko Iwasaki日期:08/20/2019,浏览量:47,Q&A: 0
    Endogenous retroviruses (ERV) are transposable retroelements that form ~10% of the murine genome and whose family members are differentially expressed throughout embryogenesis. However, precise regulation of ERV in germ cells remains unclear. To ...
    Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay
    果蝇翅膀成虫盘体内培养和弗林蛋白酶抑制剂检测
    作者:Alex Sohr, Lijuan Du and Sougata Roy日期:08/20/2019,浏览量:34,Q&A: 0
    Furin is an evolutionarily conserved proprotein convertase (PC) family enzyme with a broad range of substrates that are essential for developmental, homeostatic, and disease pathways. Classical genetic approaches and in vitro biochemical or ...
    Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
    整体原位杂交检测mRNA并提取DNA检测斑马鱼胚胎的基因分型
    作者:Rachna Narayanan and Andrew C. Oates日期:03/20/2019,浏览量:2012,Q&A: 0
    In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes ...
    Application of a Modified Smart-seq2 Sample Preparation Protocol for Rare Cell Full-length Single-cell mRNA Sequencing to Mouse Oocytes
    一种改良的Smart-seq2样品制备方案在小鼠卵母细胞中应用稀有细胞全长单细胞mRNA测序
    作者:Rebecca S. Treger, Scott D. Pope, Xiaojun Xing and Akiko Iwasaki日期:08/20/2019,浏览量:47,Q&A: 0
    [Abstract] Endogenous retroviruses (ERV) are transposable retroelements that form ~10% of the murine genome and whose family members are differentially expressed throughout embryogenesis. However, precise regulation of ERV in germ cells remains unclear. To investigate ERV expression in oocytes, we adapted a single-cell mRNA-sequencing library preparation ...
    Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay
    果蝇翅膀成虫盘体内培养和弗林蛋白酶抑制剂检测
    作者:Alex Sohr, Lijuan Du and Sougata Roy日期:08/20/2019,浏览量:34,Q&A: 0
    [Abstract] Furin is an evolutionarily conserved proprotein convertase (PC) family enzyme with a broad range of substrates that are essential for developmental, homeostatic, and disease pathways. Classical genetic approaches and in vitro biochemical or cell biological assays identified that precursor forms of most growth factor family proteins are ...
    Lipid-exchange Rate Assay for Lipid Droplet Fusion in Live Cells
    活体细胞中脂滴融合的脂类交换速率检测
    作者:Jia Wang, Boon Tin Chua, Peng Li and Feng-Jung Chen日期:07/20/2019,浏览量:482,Q&A: 0
    [Abstract] Lipid droplets (LDs) are central organelles in maintaining lipid homeostasis. Defective LD growth often results in the development of metabolic disorders. LD fusion and growth mediated by cell death–inducing DNA fragmentation factor alpha (DFFA)-like effector (CIDE) family proteins are crucial for various biological processes including unilocular ...
    CRISPR/Cas9 + AAV-mediated Intra-embryonic Gene Knocking in Mice
    小鼠体内由CRISPR / Cas9 + AAV介导的胚胎内基因敲除
    作者:Naoaki Mizuno, Eiji Mizutani, Hideyuki Sato, Mariko Kasai, Hiromitsu Nakauchi and Tomoyuki Yamaguchi日期:07/05/2019,浏览量:682,Q&A: 0
    [Abstract] Intra-embryo genome editing by CRISPR/Cas9 has enabled rapid generation of gene knockout animals. However, large fragment knock-in directly into embryos’ genome is still difficult, especially without microinjection of donor DNA. Viral vectors are good transporters of knock-in donor DNA for cell lines, but seemed unsuitable for pre-implantation ...
    Determining Oxidative Damage by Lipid Peroxidation Assay in Rat Serum
    利用脂质过氧化测定检测大鼠血清的氧化损伤
    作者:Qian Tang, Yu-Wen Su and Cory J. Xian日期:06/20/2019,浏览量:601,Q&A: 0
    [Abstract] It has been well-established that malondialdehyde (MDA), which is generated during the process of lipid peroxidation, is a commonly known biomarker for oxidative stress. Therefore, the serum levels of MDA are detected by using the lipid peroxidation assay with commercially available kit to determine the induction of oxidative stress in rat models.
    Simultaneous Fluorescent Recordings of Extracellular ATP and Intracellular Calcium in Mammalian Cells
    哺乳动物细胞胞外ATP和胞内钙离子的协同荧光记录
    作者:Nicholas Mikolajewicz and Svetlana V. Komarova日期:05/20/2019,浏览量:998,Q&A: 0
    [Abstract] Extracellular ATP is a potent signaling molecule that stimulates intracellular calcium responses through purinergic (P2) receptors in mammalian cells. While extracellular ATP and intracellular calcium can be measured separately, simultaneous monitoring can offer additional insights into P2 receptor physiology. This protocol takes advantage of the ...
    Quantification of the Humidity Effect on HR by Ion Leakage Assay
    离子渗漏实验定量分析湿度对超敏反应的影响
    作者:Musoki Mwimba and Xinnian Dong日期:04/05/2019,浏览量:820,Q&A: 0
    [Abstract] We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the ...
    Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
    整体原位杂交检测mRNA并提取DNA检测斑马鱼胚胎的基因分型
    作者:Rachna Narayanan and Andrew C. Oates日期:03/20/2019,浏览量:2012,Q&A: 0
    [Abstract] In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non-radioactive labels that can be detected by immunohistochemistry and coupled to ...
    An Improved Staining Method for Low Signal LacZ Reporter Detection in Mouse Embryos
    一种改进的可用于检测小鼠胚胎弱LacZ报告信号的染色法
    作者:Xiaopeng Shen, Feng Xu, Shen Wu, Meng Li, Jingyi Zhang, Rui Liang and Yu Liu日期:03/05/2019,浏览量:988,Q&A: 0
    [Abstract] In many fields of biology, especially in the field of developmental biology, LacZ reporter staining is an approach used to monitor gene expression patterns. In the LacZ reporter system, the LacZ gene is inserted in the endogenous location of the target gene via gene knock-in or by constructing a transgenic cassette in ...
    High-resolution Immunoelectron Microscopy Techniques for Revealing Distinct Subcellular Type 1 Cannabinoid Receptor Domains in Brain
    检测脑中不同亚细胞1型大麻素受体结构域的高分辨率免疫电镜技术
    作者:Nagore Puente, Itziar Bonilla-Del Río, Svein Achicallende, Patrick C. Nahirney and Pedro Grandes日期:01/20/2019,浏览量:1472,Q&A: 0
    [Abstract] Activation of type 1 cannabinoid (CB1) receptors by endogenous, exogenous (cannabis derivatives) or synthetic cannabinoids (i.e., CP 55.940, Win-2) has a wide variety of behavioral effects due to the presence of CB1 receptors in the brain. In situ hybridization and immunohistochemical techniques have been ...