植物科学

分类

    现刊
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    细胞壁孔隙的荧光猝灭显微镜无创定量分析
    作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:30,Q&A: 0
    All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the ...
    Peptide Feeding and Mechanical Wounding for Tomato Seedlings
    番茄幼苗的肽饲喂和机械伤害
    作者:Lei Wang, Yan Wang and Georg Felix日期:03/20/2019,浏览量:1338,Q&A: 0
    Plants need to respond appropriately to wounding and herbivorous insects. Peptide signals have been implicated in local and systemic induction of appropriate plant defense responses. To study these peptide signals and their perception in host ...
    Measuring and Imaging the Soil-root-water System with a Light Transmission 2D Technique
    利用透光二维技术对土壤-根-水系统进行测定与成像
    作者:Claude Doussan and Emmanuelle Garrigues日期:03/20/2019,浏览量:1169,Q&A: 0
    Improving crops against water deficits requires a better understanding of plant root system functioning. This requires a better knowledge of the water uptake process and to address the influence of root system architecture or root physiological ...
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    细胞壁孔隙的荧光猝灭显微镜无创定量分析
    作者:Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche日期:08/20/2019,浏览量:30,Q&A: 0
    [Abstract] All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the cell and its surroundings. Despite its name, the cell wall is a flexible, dynamic structure. ...
    Tensile Testing Assay for the Measurement of Tissue Stiffness in Arabidopsis Inflorescence Stem
    拉伸强度测试用于检测拟南芥花序轴组织刚度
    作者:Kouki Yoshida, Shingo Sakamoto and Nobutaka Mitsuda日期:08/05/2019,浏览量:406,Q&A: 0
    [Abstract] Lignocellulosic biomass is a versatile renewable resource for fuels, buildings, crafts, and biomaterials. Strategies of molecularly designing lignocellulose for industrial application has been developed by the discoveries of novel genes after the screenings of various mutants and transformed lines of Arabidopsis whose cell walls could be ...
    Measuring Protein Half-life in Arabidopsis thaliana
    拟南芥蛋白半衰期的检测
    作者:Hongqing Guo and Yanhai Yin日期:08/05/2019,浏览量:200,Q&A: 0
    [Abstract] Post-translational modifications play important roles in controlling protein function and can lead to altered protein stability. Protein stability can be determined after treatment with the protein synthesis inhibitor Cycloheximide. Cycloheximide is a translational inhibitor that inhibits protein synthesis via cytoplasmic ribosomes. Here we ...
    Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association
    蓝甲醇衍生物作为丛枝菌根真菌与植物共生标志物的定量研究
    作者:Elisabeth Mindt, Ming Wang, Martin Schäfer, Rayko Halitschke and Ian T. Baldwin日期:07/20/2019,浏览量:616,Q&A: 0
    [Abstract] Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, ...
    Isolation of Powdery Mildew Haustoria from Infected Barley
    感染大麦中白粉病吸器的分离提取
    作者:Linhan Li, Benjamin Collier and Pietro D. Spanu日期:07/20/2019,浏览量:579,Q&A: 0
    [Abstract] Blumeria graminis is a fungus that causes powdery mildews on grasses, such as barley. Investigations of this pathogen present many challenges due to its obligate biotrophic nature. This means that the fungus can only grow in the presence of a living host plant. B. graminis forms epiphytic mycelia on the plant surface and feeding ...
    Transient Expression Assay in Strawberry Fruits
    草莓果实中的瞬时表达检测
    作者:Mengting Pi, Qi Gao and Chunying Kang日期:06/05/2019,浏览量:773,Q&A: 0
    [Abstract] Strawberry, including the woodland strawberry Fragaria vesca (2x) and the cultivated strawberry (Fragaria × ananassa, 8x), has emerged as a model system for studying fruit development and ripening. Transient expression provides a quick assay for gene functions or gene interactions. In strawberry, ...
    Simple Method to Determine Protein Redox State in Arabidopsis thaliana
    一种确定拟南芥蛋白氧化还原状态的简单方法
    作者:Keisuke Yoshida and Toru Hisabori日期:06/05/2019,浏览量:888,Q&A: 0
    [Abstract] Thiol-based redox regulation is a posttranslational protein modification that plays a key role in many biological aspects. To understand its regulatory functions, we need a method to directly assess protein redox state in vivo. Here we present a simple procedure to determine protein redox state in a model plant Arabidopsis thaliana ...
    An Adjustable Protocol to Analyze Chemical Profiles of Non-sterile Rhizosphere Soil
    一种可调整的用于分析研究非无菌根际土壤化学特性的方法
    作者:Alex Williams, Jurriaan Ton and Pierre Pétriacq日期:05/20/2019,浏览量:1095,Q&A: 0
    [Abstract] The analysis of chemical diversity in non-sterile rhizosphere soil has been a pressing methodological challenge for years. Rhizosphere-enriched chemicals (i.e., rhizochemicals) include root exudation chemicals, (microbial) breakdown products thereof, and de novo produced metabolites by rhizosphere-inhabiting microbes, all of ...
    An Improved Bioassay to Study Arabidopsis Induced Systemic Resistance (ISR) Against Bacterial Pathogens and Insect Pests
    一种的可用于检测拟南芥中细菌性病原体和害虫诱导的系统抗性的改进生物学方法
    作者:Nicolás M. Cecchini, Yi Song, Suruchi Roychoudhry, Jean T. Greenberg and Cara H. Haney日期:05/20/2019,浏览量:1336,Q&A: 0
    [Abstract] The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas ...
    Analysis of Indole-3-acetic Acid (IAA) Production in Klebsiella by LC-MS/MS and the Salkowski Method
    利用液相色谱-串联质谱法和萨尔科夫斯基氏法分析克雷伯菌中吲哚乙酸的产生
    作者:Shraddha Gang, Sheetal Sharma, Meenu Saraf, Martin Buck and Jörg Schumacher日期:05/05/2019,浏览量:1144,Q&A: 0
    [Abstract] Many rhizobacteria isolated from plant rhizosphere produce various phytohormones in the form of secondary metabolites, the most common of which is Indole-3-acetic acid (IAA). Here, we detail analytical protocols of IAA detection and quantification, in vitro and in situ, as recently applied to Klebsiella SGM 81, a ...