植物科学

分类

    现刊
    Construction of Antisense RNA-mediated Gene Knock-down Strains in the Cyanobacterium Anabaena sp. PCC 7120
    反义RNA介导的鱼腥藻PCC 7120基因敲除株的构建
    作者:Amit Srivastava, Anand Ballal, Karl Forchhammer and Anil Kumar Tripathi日期:02/20/2020,浏览量:85,Q&A: 0
    Anabaena sp. PCC 7120 (hereafter Anabaena) is a model cyanobacterium to study nitrogen fixation, cellular differentiation and several other key biological functions that are analogous in plants. As with any other organism, many ...
    Construction of Antisense RNA-mediated Gene Knock-down Strains in the Cyanobacterium Anabaena sp. PCC 7120
    反义RNA介导的鱼腥藻PCC 7120基因敲除株的构建
    作者:Amit Srivastava, Anand Ballal, Karl Forchhammer and Anil Kumar Tripathi日期:02/20/2020,浏览量:85,Q&A: 0
    [Abstract] Anabaena sp. PCC 7120 (hereafter Anabaena) is a model cyanobacterium to study nitrogen fixation, cellular differentiation and several other key biological functions that are analogous in plants. As with any other organism, many genes in Anabaena encode an essential life function and hence cannot be deleted, causing a ...
    Competition Assays to Quantify the Effect of Biocontrol Yeasts against Plant Pathogenic Fungi on Fruits
    竞争分析以量化酵母菌对水果上植物病原真菌的生防作用
    作者:Electine Magoye, Melanie Pfister, Maja Hilber-Bodmer and Florian M. Freimoser日期:02/05/2020,浏览量:236,Q&A: 0
    [Abstract] Yeasts such as Aureobasidium pullulans are unicellular fungi that occur in all environments and play important roles in biotechnology, medicine, food and beverage production, research, and agriculture. In the latter, yeasts are explored as biocontrol agents for the control of plant pathogenic fungi (e.g., Botrytis cinerea, ...
    Sulfatase Assay to Determine Influence of Plants on Microbial Activity in Soil
    硫酸酯酶法测定植物对土壤微生物活性的影响
    作者:Anna Koprivova, Achim Schmalenberger and Stanislav Kopriva日期:01/20/2020,浏览量:521,Q&A: 0
    [Abstract] Sulfatase activity is often used as a measure of the activity of soil microorganisms. It is thus a suitable tool to investigate the response of microbes to plants. Here we present a method to determine the influence of various Arabidopsis genotypes on the function of soil microbiota using the sulfatase as a quantitative measure. We grew ...
    Visualization of Actin Organization and Quantification in Fixed Arabidopsis Pollen Grains and Tubes
    固定拟南芥花粉粒及花粉管中肌动蛋白组织可视化及定量分析
    作者:Xiaolu Qu, Qiannan Wang, Haiyan Wang and Shanjin Huang日期:01/05/2020,浏览量:314,Q&A: 0
    [Abstract] Although it is widely accepted that actin plays an important role in regulating pollen germination and pollen tube growth, how actin exactly performs functions remains incompletely understood. As the function of actin is dictated by its spatial organization, it is the key to reveal how exactly actin distributes in space in pollen cells. Here we ...
    Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay
    叶绿体NAD激酶活性及全组织NAD激酶活性测定
    作者:Yuuma Ishikawa, Maki Kawai-Yamada and Shin-nosuke Hashida日期:01/05/2020,浏览量:391,Q&A: 0
    [Abstract] Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP. The NAD kinase responds to various environmental stimuli and its activity is regulated via various regulatory pathways, such as Ca2+-dependent and redox-dependent signals. Conventional in ...
    Analysis of Monosaccharides from Arabidopsis Seed Mucilage and Whole Seeds Using HPAEC-PAD
    利用HPAEC-PAD进行拟南芥种子黏液和整粒种子中单糖的测定
    作者:Gillian H. Dean, Kresimir Sola, Faride Unda, Shawn D. Mansfield and George W. Haughn日期:12/20/2019,浏览量:529,Q&A: 0
    [Abstract] Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development. When mature seeds are hydrated, mucilage extrudes to form a gelatinous capsule around the seed. Determining the monosaccharide composition of ...
    Localizing Genome Segments and Protein Products of a Multipartite Virus in Host Plant Cells
    宿主植物细胞中复合病毒的基因组片段和蛋白质产物的定位
    作者:Marie-Stephanie Vernerey, Elodie Pirolles, Stephane Blanc and Anne Sicard日期:12/05/2019,浏览量:558,Q&A: 0
    [Abstract] A founding paradigm in virology is that the spatial unit of the viral replication cycle is an individual cell. This concept applied to multipartite viruses–which have a genome composed of two or more nucleic acid segments, each individually encapsulated–implies that all segments constituting a viral genome need to coinfect the same host cell for ...
    Method for Assessing Virulence of Colletotrichum higginsianum on Arabidopsis thaliana Leaves Using Automated Lesion Area Detection and Measurement
    应用损伤面积的自动化检测和测量评价炭疽菌对拟南芥叶片致病性的方法
    作者:Ayako Tsushima, Pamela Gan and Ken Shirasu日期:11/20/2019,浏览量:1137,Q&A: 0
    [Abstract] The plant pathogenic fungus, Colletotrichum higginsianum is widely used to understand infection mechanisms, as it infects the model plant Arabidopsis thaliana. To determine the virulence of C. higginsianum, several methods have been developed, such as disease reaction scoring, lesion measurement, entry rate assays, and ...
    Cell Wall Compositional Analysis of Rice Culms
    水稻茎杆的细胞壁组成分析
    作者:Lanjun Zhang, Baocai Zhang and Yihua Zhou日期:10/20/2019,浏览量:651,Q&A: 0
    [Abstract] The plant cell wall is a complicated network that is mainly constituted of polysaccharides, such as cellulose, hemicellulose and pectin. Many noncellulosic polysaccharides are further acetylated, which confers these polymers flexible physicochemical properties. Due to the significance of cell wall in plant growth and development, the analytic ...
    Non-aqueous Fractionation (NAF) for Metabolite Analysis in Subcellular Compartments of Arabidopsis Leaf Tissues
    非水体系分离(NAF)用于拟南芥叶组织亚细胞组分的代谢研究
    作者:David B. Medeiros, Stéphanie Arrivault, Jessica Alpers, Alisdair R. Fernie and Fayezeh Arabi日期:10/20/2019,浏览量:624,Q&A: 0
    [Abstract] The accurate determination of metabolite distribution in subcellular compartments is still challenging in plant science. Various methodologies, such as fluorescence resonance energy transfer-based technology, nuclear magnetic resonance spectroscopy and protoplast fractionation allow the study of metabolite compartmentation. However, large changes ...