植物科学

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    现刊
    Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
    农杆菌介导的优良籼稻品种Komboka的高效转化
    Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of ...
    An Efficient Inoculation Technique to Assess the Pathogenicity of Pantoea Species Associated to Bacterial Blight of Rice
    一种用于评估水稻白叶枯病泛菌致病性的高效接种技术
    作者:Kossi Kini, Raoul Agnimonhan, Issa Wonni and Drissa Silue日期:09/05/2020,浏览量:443,Q&A: 0
    Bacteria blight diseases of rice due to several genera of pathogenic bacteria are one of the major constraints worldwide for rice production. The disease can be best managed through host plant resistance sources. For most of these bacteria such as ...
    Ratiometric Measurement of Protein Abundance after Transient Expression of a Transgene in Nicotiana benthamiana
    转基因烟草瞬时表达后蛋白质丰度的比例计量测定
    作者:Aashima Khosla and David C. Nelson日期:09/05/2020,浏览量:477,Q&A: 0
    Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein ...
    Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
    农杆菌介导的优良籼稻品种Komboka的高效转化
    [Abstract] Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of the world’s population. As a result, numerous transformation methods have been developed for both ...
    An Efficient Inoculation Technique to Assess the Pathogenicity of Pantoea Species Associated to Bacterial Blight of Rice
    一种用于评估水稻白叶枯病泛菌致病性的高效接种技术
    作者:Kossi Kini, Raoul Agnimonhan, Issa Wonni and Drissa Silue日期:09/05/2020,浏览量:443,Q&A: 0
    [Abstract] Bacteria blight diseases of rice due to several genera of pathogenic bacteria are one of the major constraints worldwide for rice production. The disease can be best managed through host plant resistance sources. For most of these bacteria such as Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, ...
    Ratiometric Measurement of Protein Abundance after Transient Expression of a Transgene in Nicotiana benthamiana
    转基因烟草瞬时表达后蛋白质丰度的比例计量测定
    作者:Aashima Khosla and David C. Nelson日期:09/05/2020,浏览量:477,Q&A: 0
    [Abstract] Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein fused to a second reporter. Both fusion proteins are encoded on a single transcript but are ...
    The ATPase Activity of Escherichia coli Expressed AAA+-ATPase Protein
    大肠杆菌表达的AAA + -ATPase蛋白的ATPase活性
    作者:Amita Kaundal, Vemanna S. Ramu and Kirankumar S. Mysore日期:08/05/2020,浏览量:572,Q&A: 0
    [Abstract] ATPases are the enzymes that breakdown ATP to ADP and release inorganic phosphate (Pi). Here we provide a detailed protocol to determine the ATPase activity of a recombinant AAA+-ATPase protein (GENERAL CONTROL NON-REPRESSIBLE-4 [GCN4]) by spectrophotometric absorption at 360 nm to measure the accumulated inorganic phosphate. In ...
    Generation and in Planta Functional Analysis of Potato Virus Y mutants
    马铃薯Y病毒突变体的产生及其在植物中的功能分析
    [Abstract] Potato virus Y (PVY), the type member of the genus Potyvirus (family Potyviridae), is the most widespread virus affecting potato and is included in the top five most economically detrimental plant viruses. Recently, the structure of the PVY virion has been determined by cryo-electron microscopy, which has opened the doors to ...
    Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes
    大分子直接导入水稻卵细胞和受精卵的基因表达和基因组编辑系统
    作者:Erika Toda and Takashi Okamoto日期:07/20/2020,浏览量:475,Q&A: 0
    [Abstract] Polyethylene glycol calcium (PEG-Ca2+)-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered into protoplasts derived from somatic tissues or calli via PEG-Ca2+ transfection. To ...
    RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings
    RT-qPCR法测定拟南芥幼苗RNA稳定性
    作者:Tianran Jia and Brandon H. Le日期:07/20/2020,浏览量:376,Q&A: 0
    [Abstract] Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions. mRNA stability can be measured indirectly after transcription inhibition. This protocol described a rapid and sensitive ...
    In vitro Assessment of Pathogen Effector Binding to Host Proteins by Surface Plasmon Resonance
    利用表面等离子体共振技术评价病原菌与宿主蛋白的结合
    作者:Marina Franceschetti, Mark J. Banfield, Clare E. M. Stevenson and Juan Carlos De la Concepcion日期:07/05/2020,浏览量:1137,Q&A: 0
    [Abstract] The mechanisms of virulence and immunity are often governed by molecular interactions between pathogens and host proteins. The study of these interactions has major implications on understanding virulence activities, and how the host immune system recognizes the presence of pathogens to initiate an immune response. Frequently, the association ...
    Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids
    利用甲基化敏感扩增多态性分析野生马铃薯杂交种
    作者:Nicolás Cara, Carlos F. Marfil, María V. Bertoldi and Ricardo W. Masuelli日期:07/05/2020,浏览量:623,Q&A: 0
    [Abstract] Methylation-Sensitive Amplification Polymorphism (MSAP) is a versatile marker for analyzing DNA methylation patterns in non-model species. The implementation of this technique does not require a reference genome and makes it possible to determine the methylation status of hundreds of anonymous loci distributed throughout the genome. In addition, ...
    Maintenance and Quantitative Phenotyping of the Oomycete-plant Model Pathosystem Hyaloperonospora arabidopsidisArabidopsis
    卵菌-植物模型病理系统(活体营养型卵菌–拟南芥)的维持和定量表型分析
    作者:Fang-Yu Hwu and Martin Parniske日期:06/20/2020,浏览量:773,Q&A: 0
    [Abstract] The interaction between the host plant Arabidopsis thaliana (Arabidopsis) and the oomycete Hyaloperonospora arabidopsidis (Hpa) is an established model system for the study of an obligate biotrophic downy mildew interaction. The evaluation of the developmental success of Hpa is often based on the quantification ...