生物物理学

分类

    现刊
    A Workflow for High-pressure Freezing and Freeze Substitution of the Caenorhabditis elegans Embryo for Ultrastructural Analysis by Conventional and Volume Electron Microscopy
    用常规和体积电子显微镜对秀丽隐杆线虫胚胎进行超微结构分析的高压冷冻和冷冻替代的工作流程
    作者:Mohammad M. Rahman, Irene Y. Chang, Orna Cohen-Fix and Kedar Narayan日期:04/05/2021,浏览量:226,Q&A: 0
    [Abstract]

    The free-living nematode Caenorhabditis elegans is a popular model system for studying developmental biology. Here we describe a detailed protocol to high-pressure freeze the C. elegans embryo (either ex vivo after dissection, or within the intact worm) followed by quick freeze substitution. Processed samples are suitable for ultrastructural

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    Developing Biohybrid Robotic Jellyfish (Aurelia aurita) for Free-swimming Tests in the Laboratory and in the Field
    开发的生物合成机器人水母在室内和室外的自由游泳测试
    作者:Nicole W. Xu, James P. Townsend, John H. Costello, Sean P. Colin, Brad J. Gemmell and John O. Dabiri日期:04/05/2021,浏览量:208,Q&A: 0
    [Abstract]

    Biohybrid robotics is a growing field that incorporates both live tissues and engineered materials to build robots that address current limitations in robots, including high power consumption and low damage tolerance. One approach is to use microelectronics to enhance whole organisms, which has previously been achieved to control the locomotion of

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    FRET-based Microscopy Assay to Measure Activity of Membrane Amino Acid Transporters with Single-transporter Resolution
    用荧光共振能量转移的显微技术测定单转运体膜氨基酸转运体的活性
    [Abstract]

    Secondary active transporters reside in cell membranes transporting polar solutes like amino acids against steep concentration gradients, using electrochemical gradients of ions as energy sources. Commonly, ensemble-based measurements of radiolabeled substrate uptakes or transport currents inform on kinetic parameters of transporters. Here we

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    Investigate Synaptic Vesicles Mobility in Neuronal Culture Axons by FRAP Imaging
    用FRAP成像技术研究神经元轴突中突触囊泡的移动
    作者:Xiao Min Zhang, Fabrice P. Cordelieres and Etienne Herzog日期:03/20/2021,浏览量:603,Q&A: 0
    [Abstract]

    Synaptic vesicles (SVs) are clustered in the presynaptic terminals and consistently trafficking along axons. Based on their release features, SVs are classified into different “pools”. Imaging of SVs that are traveling among multiple presynaptic terminals has helped define a new pool named “SV super-pool”. Here we describe

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    Characterize the Interaction of the DNA Helicase PriA with the Stalled DNA Replication Fork Using Atomic Force Microscopy
    用原子力显微镜描述DNA解旋酶PriA与停止的DNA复制叉的相互作用
    作者:Yaqing Wang, Zhiqiang Sun, Piero R. Bianco and Yuri L. Lyubchenko日期:03/05/2021,浏览量:943,Q&A: 0
    [Abstract]

    In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. ssDNA-binding protein (SSB) is typically present at the abandoned forks,

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    Simultaneous Imaging of Single Protein Size, Charge, and Binding Using A Protein Oscillation Approach
    利用蛋白质振荡方法同时成像单个蛋白质的大小、电荷和结合状态
    作者:Guangzhong Ma, Zijian Wan and Shaopeng Wang日期:03/05/2021,浏览量:1235,Q&A: 0
    [Abstract]

    Electrophoresis and Western blot are important tools in protein research for detection and identification of proteins. These traditional techniques separate the proteins based on size and charge differences and identify the proteins by antibody binding. Over the past decade, the emergence of single-molecule techniques has shown great potential in

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    Automated Analysis of Cerebrospinal Fluid Flow and Motile Cilia Properties in The Central Canal of Zebrafish Embryos
    斑马鱼胚胎中央管中脑脊液流动和运动纤毛特性的自动分析
    作者:Olivier Thouvenin, Yasmine Cantaut-Belarif, Ludovic Keiser, François Gallaire and Claire Wyart日期:03/05/2021,浏览量:773,Q&A: 0
    [Abstract]

    Circulation of cerebrospinal fluid (CSF) plays an important role during development. In zebrafish embryo, the flow of CSF has been found to be bidirectional in the central canal of the spinal cord. In order to compare conditions and genetic mutants across each other, we recently automated the quantification of the velocity profile of exogenous

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    Live Cell FRET Analysis of the Conformational Changes of Human P-glycoprotein
    人P-糖蛋白构象变化的活细胞FRET分析
    作者:Ryota Futamata, Noriyuki Kioka and Kazumitsu Ueda日期:02/20/2021,浏览量:1059,Q&A: 0
    [Abstract]

    The molecular mechanisms of P-glycoprotein (P-gp; also known as MDR1 or ABCB1) have been mainly investigated using artificial membranes such as lipid-detergent mixed micelles, artificial lipid bilayers, and membrane vesicles derived from cultured cells. Although these in vitro experiments help illustrate details about the molecular mechanisms of

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    Expression and Purification of Yeast-derived GPCR, Gα and Gβγ Subunits for Structural and Dynamic Studies
    重组酵母的GPCR、Gα和Gβγ亚基的表达和纯化,用于结构和动力学研究
    作者:Wenjie Zhao, Xudong Wang and Libin Ye日期:02/20/2021,浏览量:1396,Q&A: 0
    [Abstract]

    In the last several years, as evidence of a surged number of GPCR-G complex structures, the expressions of GPCRs and G proteins for structural biology have achieved tremendous successes, mostly in insect and mammalian cell systems, resulting in more than 370 structures of over 70 GPCRs have been resolved. However, the challenge remains,

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    Resolving Structural Changes of Photoreceptors in Living Escherichia coli via In-cell Infrared Difference Spectroscopy
    利用细胞内红外差谱技术分析活大肠杆菌光感受器的结构变化
    作者:Lukas Goett-Zink, Jessica L. Klocke and Tilman Kottke日期:02/05/2021,浏览量:615,Q&A: 0
    [Abstract]

    Several in-cell spectroscopic techniques have been developed recently to investigate the structure and mechanism of proteins in their native environment. Conditions in vivo differ dramatically from those selected for in vitro experiments. Accordingly, the cellular environment can affect the protein mechanism for example by molecular crowding or

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