生物物理学

分类

    现刊
    Electrophysiological Properties of Neurons: Current-Clamp Recordings in Mouse Brain Slices and Firing-Pattern Analysis
    神经元的电生理特性:小鼠脑切片中的电流钳记录和放电模式分析
    作者:Elina Nagaeva, Ivan Zubarev and Esa R. Korpi日期:06/20/2021,浏览量:0,Q&A: 0

    Characterization of an electrically active cell, such as a neuron, demands measurement of its electrical properties. Due to differences in gene activation, location, innervation patterns, and functions, the millions of neurons in the mammalian brain

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    Preparation of Doublet Microtubule Fraction for Single Particle Cryo-electron Microscopy
    单粒子低温电子显微镜双态微管馏分的制备
    作者:Corbin Black, Daniel Chen Dai, Katya Peri, Muneyoshi Ichikawa and Khanh Huy Bui日期:06/05/2021,浏览量:404,Q&A: 0

    Over the years, studying the ultrastructure of the eukaryotic cilia/flagella using electron microscopy (EM) has contributed significantly toward our understanding of ciliary function. Major complexes in the cilia, such as inner and outer dynein

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    Electrophysiological Properties of Neurons: Current-Clamp Recordings in Mouse Brain Slices and Firing-Pattern Analysis
    神经元的电生理特性:小鼠脑切片中的电流钳记录和放电模式分析
    作者:Elina Nagaeva, Ivan Zubarev and Esa R. Korpi日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    Characterization of an electrically active cell, such as a neuron, demands measurement of its electrical properties. Due to differences in gene activation, location, innervation patterns, and functions, the millions of neurons in the mammalian brain are tremendously diverse in their membrane characteristics and abilities to generate action

    ...
    Preparation of Doublet Microtubule Fraction for Single Particle Cryo-electron Microscopy
    单粒子低温电子显微镜双态微管馏分的制备
    作者:Corbin Black, Daniel Chen Dai, Katya Peri, Muneyoshi Ichikawa and Khanh Huy Bui日期:06/05/2021,浏览量:404,Q&A: 0
    [Abstract]

    Over the years, studying the ultrastructure of the eukaryotic cilia/flagella using electron microscopy (EM) has contributed significantly toward our understanding of ciliary function. Major complexes in the cilia, such as inner and outer dynein arms, radial spokes, and dynein regulatory complexes, were originally discovered by EM. Classical

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    Surface Engineering and Multimodal Imaging of Multistage Delivery Vectors in Metastatic Breast Cancer
    转移性乳腺癌多期转运载体的表面工程和多模态成像
    作者:Shreya Goel, Mauro Ferrari and Haifa Shen日期:05/20/2021,浏览量:804,Q&A: 0
    [Abstract]

    The design of effective nanoformulations that target metastatic breast cancers is challenging due to a lack of competent imaging and image analysis protocols that can capture the interactions between the injected nanoparticles and metastatic lesions. Here, we describe the integration of in vivo whole-body PET-CT with high temporal resolution, ex

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    Single-Molecule Studies of Membrane Receptors from Brain Region Specific Nanovesicles
    脑区特异性纳米泡膜受体的单分子研究
    作者:Surya P. Aryal, Xu Fu, Abdullah A. Masud, Khaga R. Neupane and Christopher I. Richards日期:05/20/2021,浏览量:914,Q&A: 0
    [Abstract]

    Single molecule imaging and spectroscopy are powerful techniques for the study of a wide range of biological processes including protein assembly and trafficking. However, in vivo single molecule imaging of biomolecules has been challenging because of difficulties associated with sample preparation and technical challenges associated with

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    Spin Labeling of RNA Using “Click” Chemistry for Coarse-grained Structure Determination via Pulsed Electron-electron Double Resonance Spectroscopy
    利用脉冲电子-电子双共振光谱测定粗粒结构的“点击”化学的RNA自旋标记
    作者:Maria F. Vicino, Tobias Hett and Olav Schiemann日期:05/05/2021,浏览量:630,Q&A: 0
    [Abstract]

    Understanding the function of oligonucleotides on a molecular level requires methods for studying their structure, conformational changes, and internal dynamics. Various biophysical methods exist to achieve this, including the whole toolbox of Electron Paramagnetic Resonance (EPR or ESR) spectroscopy. An EPR method widely used in this regard is

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    A Workflow for High-pressure Freezing and Freeze Substitution of the Caenorhabditis elegans Embryo for Ultrastructural Analysis by Conventional and Volume Electron Microscopy
    用常规和体积电子显微镜对秀丽隐杆线虫胚胎进行超微结构分析的高压冷冻和冷冻替代的工作流程
    作者:Mohammad M. Rahman, Irene Y. Chang, Orna Cohen-Fix and Kedar Narayan日期:04/05/2021,浏览量:661,Q&A: 0
    [Abstract]

    The free-living nematode Caenorhabditis elegans is a popular model system for studying developmental biology. Here we describe a detailed protocol to high-pressure freeze the C. elegans embryo (either ex vivo after dissection, or within the intact worm) followed by quick freeze substitution. Processed samples are suitable for ultrastructural

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    Developing Biohybrid Robotic Jellyfish (Aurelia aurita) for Free-swimming Tests in the Laboratory and in the Field
    开发的生物合成机器人水母在室内和室外的自由游泳测试
    作者:Nicole W. Xu, James P. Townsend, John H. Costello, Sean P. Colin, Brad J. Gemmell and John O. Dabiri日期:04/05/2021,浏览量:480,Q&A: 0
    [Abstract]

    Biohybrid robotics is a growing field that incorporates both live tissues and engineered materials to build robots that address current limitations in robots, including high power consumption and low damage tolerance. One approach is to use microelectronics to enhance whole organisms, which has previously been achieved to control the locomotion of

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    FRET-based Microscopy Assay to Measure Activity of Membrane Amino Acid Transporters with Single-transporter Resolution
    用荧光共振能量转移的显微技术测定单转运体膜氨基酸转运体的活性
    [Abstract]

    Secondary active transporters reside in cell membranes transporting polar solutes like amino acids against steep concentration gradients, using electrochemical gradients of ions as energy sources. Commonly, ensemble-based measurements of radiolabeled substrate uptakes or transport currents inform on kinetic parameters of transporters. Here we

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    Investigate Synaptic Vesicles Mobility in Neuronal Culture Axons by FRAP Imaging
    用FRAP成像技术研究神经元轴突中突触囊泡的移动
    作者:Xiao Min Zhang, Fabrice P. Cordelieres and Etienne Herzog日期:03/20/2021,浏览量:1026,Q&A: 0
    [Abstract]

    Synaptic vesicles (SVs) are clustered in the presynaptic terminals and consistently trafficking along axons. Based on their release features, SVs are classified into different “pools”. Imaging of SVs that are traveling among multiple presynaptic terminals has helped define a new pool named “SV super-pool”. Here we describe

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    Characterize the Interaction of the DNA Helicase PriA with the Stalled DNA Replication Fork Using Atomic Force Microscopy
    用原子力显微镜描述DNA解旋酶PriA与停止的DNA复制叉的相互作用
    作者:Yaqing Wang, Zhiqiang Sun, Piero R. Bianco and Yuri L. Lyubchenko日期:03/05/2021,浏览量:1348,Q&A: 0
    [Abstract]

    In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. ssDNA-binding protein (SSB) is typically present at the abandoned forks,

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