干细胞

分类

    现刊
    Single or Repeated Ablation of Mouse Olfactory Epithelium by Methimazole
    甲巯咪唑单次或多次消融小鼠嗅上皮细胞
    作者:Sofia Håglin, Staffan Bohm and Anna Berghard日期:04/20/2021,浏览量:69,Q&A: 0

    Odor-detecting olfactory sensory neurons residing in the nasal olfactory epithelium (OE) are the only neurons in direct contact with the external environment. As a result, these neurons are subjected to chemical, physical, and infectious insults,

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    Single or Repeated Ablation of Mouse Olfactory Epithelium by Methimazole
    甲巯咪唑单次或多次消融小鼠嗅上皮细胞
    作者:Sofia Håglin, Staffan Bohm and Anna Berghard日期:04/20/2021,浏览量:69,Q&A: 0
    [Abstract]

    Odor-detecting olfactory sensory neurons residing in the nasal olfactory epithelium (OE) are the only neurons in direct contact with the external environment. As a result, these neurons are subjected to chemical, physical, and infectious insults, which may be the underlying reason why neurogenesis occurs in the OE of adult mammals. This feature

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    Generation of Human iPSC-derived Neural Progenitor Cells (NPCs) as Drug Discovery Model for Neurological and Mitochondrial Disorders
    人来源iPSC获得的神经祖细胞(NPCs)的产生作为神经和线粒体疾病的药物发现模型
    作者:Annika Zink, Pawel Lisowski and Alessandro Prigione日期:03/05/2021,浏览量:794,Q&A: 0
    [Abstract]

    The high attrition rate in drug development processes calls for additional human-based model systems. However, in the context of brain disorders, sampling live neuronal cells for compound testing is not applicable. The use of human induced pluripotent stem cells (iPSCs) has revolutionized the field of neuronal disease modeling and drug discovery.

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    Generation of the Compression-induced Dedifferentiated Adipocytes (CiDAs) Using Hypertonic Medium
    使用高渗培养基压缩诱导的去分化脂肪细胞(CiDAs)的产生
    [Abstract]

    Current methods to obtain mesenchymal stem cells (MSCs) involve sampling, culturing, and expanding of primary MSCs from adipose, bone marrow, and umbilical cord tissues. However, the drawbacks are the limited numbers of total cells in MSC pools, and their decaying stemness during in vitro expansion. As an alternative resource, recent ceiling

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    Rapid and Simplified Induction of Neural Stem/Progenitor Cells (NSCs/NPCs) and Neurons from Human Induced Pluripotent Stem Cells (hiPSCs)
    从人诱导的多能干细胞(hiPSCs)快速简便地诱导神经干/祖细胞(NSCs/NPCs)和神经元
    作者:Ryutaro Kajihara, Tadahiro Numakawa and Takumi Era日期:02/05/2021,浏览量:497,Q&A: 0
    [Abstract]

    Human induced pluripotent stem cells (iPSCs) and their progeny displaying tissue-specific characteristics have paved the way for regenerative medicine and research in various fields such as the elucidation of the pathological mechanism of diseases and the discovery of drug candidates. iPSC-derived neurons are particularly valuable as it is

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    Histological and Immunohistochemical Examination of Stem Cell Proliferation and Reepithelialization in the Wounded Skin
    损伤皮肤干细胞增殖和再生的组织学和免疫组化检测
    作者:Rupali Gund, Ravindra Zirmire, Haarshaadri J., Gaurav Kansagara and Colin Jamora日期:01/20/2021,浏览量:838,Q&A: 0
    [Abstract]

    The skin is the largest organ that protects our body from the external environment and it is constantly exposed to pathogenic insults and injury. Repair of damage to this organ is carried out by a complex process involving three overlapping phases of inflammation, proliferation and remodeling. Histological analysis of wounded skin is a convenient

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    Establishing an Adult Mouse Brain Hippocampal Organotypic Slice Culture System that Allows for Tracing and Pharmacological Manipulation of ex vivo Neurogenesis
    用于追踪和离体神经发生药理学操作的成年小鼠脑器官型海马切片培养系统的建立
    作者:Steffen Mayerl and Charles ffrench-Constant日期:01/05/2021,浏览量:798,Q&A: 0
    [Abstract]

    The function of the hippocampus depends on the process of adult hippocampal neurogenesis which underpins the exceptional neural plasticity of this structure, and is also frequently affected in CNS pathologies. Thus, manipulation of this process represents an important therapeutic goal. To identify potential strategies, organotypic adult brain

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    Isolation of Extracellular Vesicles Derived from Mesenchymal Stromal Cells by Ultracentrifugation
    超速离心法分离间充质基质细胞胞外囊泡
    [Abstract] Extracellular vesicles (EVs) are a heterogeneous group of membranous vesicles that differ on their biogenesis and release pathways, such as exosomes, microvesicles and apoptotic bodies. They are involved in cell-to-cell communication delivering signal molecules (proteins, nucleic acids, lipids, etc.) that can regulate different ...
    Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) into Osteoclasts
    人诱导多能干细胞分化破骨细胞的研究
    作者:I-Ping Chen日期:12/20/2020,浏览量:830,Q&A: 0
    [Abstract]

    Defects in bone resorption by osteoclasts result in numerous rare genetic bone disorders as well as in some common diseases such as osteoporosis or osteopetrosis. The use of hiPSC-differentiated osteoclasts opens new avenues in this research field by providing an unlimited cell source and overcoming obstacles such as unavailability of human

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    Protocol for Isolation, Stimulation and Functional Profiling of Primary and iPSC-derived Human NK Cells
    原代和iPSC来源的人类NK细胞的分离、刺激和功能分析方案
    [Abstract]

    Natural killer (NK) cells are innate immune cells, characterized by their cytotoxic capacity, and chemokine and cytokine secretion upon activation. Human NK cells are identified by CD56 expression. Circulating NK cells can be further subdivided into the CD56bright (~10%) and CD56dim NK cell subsets (~90%). NK cell-like cells can also be derived

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    Derivation of Induced Pluripotent Stem Cells from Human Fibroblasts Using a Non-integrative System in Feeder-free Conditions
    无饲养层条件下利用非整合系统从人成纤维细胞中衍生诱导多能干细胞
    作者:Alvaro A. Beltran, Sarahi G. Molina and Adriana S. Beltran日期:10/20/2020,浏览量:858,Q&A: 0
    [Abstract] Induced pluripotent stem cells (iPSCs) are genetically reprogrammed somatic cells that exhibit features identical to those of embryonic stem cells (ESCs). Multiple approaches are available to derive iPSCs, among which the Sendai virus is the most effective at reprogramming different cell types. Here we describe a rapid, efficient, safe, and ...