干细胞

分类

    现刊
    Isolation and Culture of Single Myofiber and Immunostaining of Satellite Cells from Adult C57BL/6J Mice
    成年C57BL/6J大鼠单个肌纤维的培养分离和卫星细胞的免疫染色
    作者:Shujie Chen, Hongrong Ding, Xiangping Yao and Liwei Xie日期:07/20/2019,浏览量:543,Q&A: 0
    [Abstract] Myofiber isolation followed with ex vivo culture could recapitulate and visualize satellite cells (SCs) activation, proliferation, and differentiation. This approach could be taken to understand the physiology of satellite cells and the molecular mechanism of regulatory factors, in terms of the involvement of intrinsic factors over SCs ...
    Axon-seq for in Depth Analysis of the RNA Content of Neuronal Processes
    神经元突起RNA含量的Axon-seq 深入分析
    作者:Jik Nijssen, Julio Aguila and Eva Hedlund日期:07/20/2019,浏览量:619,Q&A: 0
    [Abstract] Neuronal processes have an RNA composition that is distinct from the cell body. Therefore, to fully understand neuronal biology in health and disease we need to study both somas, dendrites and axons. Here we describe a detailed protocol of a newly refined method, Axon-seq, for RNA sequencing of axons (and dendrites) grown in isolation using single ...
    CRISPR/Cas9 + AAV-mediated Intra-embryonic Gene Knocking in Mice
    小鼠体内由CRISPR / Cas9 + AAV介导的胚胎内基因敲除
    作者:Naoaki Mizuno, Eiji Mizutani, Hideyuki Sato, Mariko Kasai, Hiromitsu Nakauchi and Tomoyuki Yamaguchi日期:07/05/2019,浏览量:682,Q&A: 0
    [Abstract] Intra-embryo genome editing by CRISPR/Cas9 has enabled rapid generation of gene knockout animals. However, large fragment knock-in directly into embryos’ genome is still difficult, especially without microinjection of donor DNA. Viral vectors are good transporters of knock-in donor DNA for cell lines, but seemed unsuitable for pre-implantation ...
    Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons
    人诱导多能干细胞 (iPSCs) 分化为前脑神经祖细胞和成熟神经元的有效模型
    [Abstract] Induced Pluripotent Stem Cells (iPSCs) are pluripotent stem cells that can be generated from somatic cells, and provide a way to model the development of neural tissues in vitro. One particularly interesting application of iPSCs is the development of neurons analogous to those found in the human forebrain. Forebrain neurons play a central ...
    An Improved Staining Method for Low Signal LacZ Reporter Detection in Mouse Embryos
    一种改进的可用于检测小鼠胚胎弱LacZ报告信号的染色法
    作者:Xiaopeng Shen, Feng Xu, Shen Wu, Meng Li, Jingyi Zhang, Rui Liang and Yu Liu日期:03/05/2019,浏览量:988,Q&A: 0
    [Abstract] In many fields of biology, especially in the field of developmental biology, LacZ reporter staining is an approach used to monitor gene expression patterns. In the LacZ reporter system, the LacZ gene is inserted in the endogenous location of the target gene via gene knock-in or by constructing a transgenic cassette in ...
    Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue
    从胚胎小鼠海马分离神经干细胞用于体外生长或植入宿主组织
    作者:Oksana Rybachuk, Olga Kopach, Tetyana Pivneva and Vitaliy Kyryk日期:02/20/2019,浏览量:1278,Q&A: 0
    [Abstract] For both stem cell research and treatment of the central nervous system disorders, neural stem/progenitor cells (NSPCs) represent an important breakthrough tool. In the expanded stem cell-based therapy use, NSPCs not only provide a powerful cell source for neural cell replacement but a useful model for developmental biology research. Despite ...
    Cell Microencapsulation and Cryopreservation with Low Molecular Weight Hyaluronan and Dimethyl Sulfoxide
    利用低分子量透明质酸和二甲基亚砜进行细胞微胶囊化和冷冻保存
    作者:H. Gurruchaga, L. Saenz del Burgo, G. Orive, R. M. Hernandez, J. Ciriza and J. L. Pedraz日期:02/20/2019,浏览量:1074,Q&A: 0
    [Abstract] Cryopreservation is commonly used for the storage of cells, tissues, organs or 3D cell-based products using ultra-low temperatures, which involves the immersion in liquid nitrogen for their long-term preservation. The cryopreservation of several microencapsulated cells is usually performed by the slow freezing with the dimethyl sulfoxide (DMSO) as ...
    Isolation of Multipotent Mesenchymal Stem Cells from Human Extraocular Muscle Tissue
    人眼外肌组织多能间充质干细胞的分离
    作者:Amit Sharma, Darilang Mawrie, Damarais Magdalene and Bithiah Grace Jaganathan日期:02/20/2019,浏览量:1178,Q&A: 0
    [Abstract] Mesenchymal stem cells (MSCs) have attracted significant attention as potential therapeutic cells to treat various diseases ranging from tissue injuries, graft versus host disease, degenerative diseases and cancer. Since the initial discovery of MSCs in the bone marrow cells, MSCs have been successfully isolated from various adult and neo-natal ...
    Quantification of Mouse Hematopoietic Progenitors’ Formation Using Time-lapse Microscopy and Image Analysis
    使用延时显微技术和图像分析定量小鼠造血祖细胞的形成
    作者:Isabelle Bergiers, Christian Tischer, Özge Vargel Bölükbaşı and Christophe Lancrin日期:01/05/2019,浏览量:1643,Q&A: 0
    [Abstract] In vitro differentiation of mouse embryonic stem cells (mESCs) towards blood cells constitutes a well-established system to study the endothelial-to-hematopoietic transition (EHT) at the onset of blood development. Assessing the emergence of small non-adherent round blood cells in the culture without disturbing it is essential to evaluate ...
    One-step Derivation of Functional Mesenchymal Stem Cells from Human Pluripotent Stem Cells
    人多能干细胞到功能性间充质干细胞的单步派生
    作者:Yan Zhou, JinQi Liao, ChongZhou Fang, CuiPing Mo, GuangQian Zhou and Yonglun Luo日期:11/20/2018,浏览量:1593,Q&A: 0
    [Abstract] Mesenchymal stem cells (MSCs) are invaluable cell sources for understanding stem cell biology and potential application in tissue engineering and regenerative medicine. The current issues of MSCs that demand to be further addressed are limited donors, tissue sources and limited capacity of ex vivo expansion. Here, we describe a simple and ...