联合作者(
Melike Çağlayan University of Florida
3 protocols

Julie K. Horton Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
1 protocol

Samuel H. Wilson
  • Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
研究方向
  • 微生物学
  • 已发表 protocol 3
已发表 protocol 3篇
In vitro Assay to Measure DNA Polymerase β Nucleotide Insertion Coupled with the DNA Ligation Reaction during Base Excision Repair
体外实验检测在碱基去除修复中聚合酶β联合DNA连接反应介导的核苷酸插入

作者:Melike Çağlayan and Samuel H. Wilson日期:06/20/2017,浏览量:6100,Q&A: 0
We previously reported that oxidized nucleotide insertion by DNA polymerase β (pol β) can confound the DNA ligation step during base excision repair (BER) (Çağlayan et al., 2017). Here, we describe a method to investigate pol β nucleotide ...
Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells
脊椎动物细胞提取物的碱基切除修复酶的酶活性检测

作者:Melike Çağlayan, Julie K. Horton and Samuel H. Wilson日期:06/05/2015,浏览量:7082,Q&A: 0
We previously reported enzymatic activity assays for the base excision repair (BER) enzymes DNA polymerase β (pol β), aprataxin (APTX), and flap endonuclease 1 (FEN1) in cell extracts from Saccharomyces cerevisiae (Çağlayan and Wilson, ...