联合作者(
Kohei Nishimura Nagoya Nagoya
1 protocol

Mariko Ariyoshi Graduate School of frontier biosciences, Osaka University
1 protocol

Masatoshi Hara Graduate School of Frontier Biosciences Osaka Univ
1 protocol

Reito Watanabe Graduate School of Frontier Biosciences Osaka Univ
1 protocol

Tatsuo Fukagawa
  • Prof.,
研究方向
  • Kinetochore: Centromere; Mitosis; chromosome segregation; Epigenetics
  • 已发表 protocol 2

教育背景

Ph. D, National Institute Genetics, 1995

实验室信息

Fukagawa Lab
http://www.fbs.osaka-u.ac.jp/labs/fukagawa/index.html

已发表 protocol 2篇
A Simple Method to Generate Super-sensitive AID (ssAID)-based Conditional Knockouts using CRISPR-based Gene Knockout in Various Vertebrate Cell Lines
小胶质细胞的分离及蛋白表达的流式细胞仪分析:避免小胶质细胞背景荧光的陷阱

作者:Kohei Nishimura and Tatsuo Fukagawa日期:07/20/2021,浏览量:713,Q&A: 0

Inducing loss of function of a target protein using methods such as gene knockout is a powerful and useful strategy for analyzing protein function in cells. In recent years, the CRISPR/Cas-9-based gene knockout technology has been widely used across

...
CENP-C Phosphorylation by CDK1 in vitro
CDK1体外磷酸化CENP-C的研究

作者:Reito Watanabe, Masatoshi Hara, Mariko Ariyoshi and Tatsuo Fukagawa日期:01/05/2021,浏览量:2134,Q&A: 0

Accurate chromosome segregation during mitosis requires the kinetochore, a large protein complex, which makes a linkage between chromosomes and spindle microtubes. An essential kinetochore component, CENP-C, is phosphorylated by Cyclin-B-Cyclin

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