干细胞

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    现刊
    Gene Expression Analysis in Stem Cell-derived Cortical Neuronal Cultures Using Multi-well SYBR Green Quantitative PCR Arrays
    使用多孔 SYBR Green 定量 PCR 阵列在干细胞衍生的皮质神经元培养物中进行基因表达分析
    作者:Vasavi Nallur Srinivasaraghavan, Faria Zafar and Birgitt Schüle日期:07/20/2022,浏览量:533,Q&A: 0
    [Abstract]

    To optimize differentiation protocols for stem cell-based in vitro modeling applications, it is essential to assess the change in gene expression during the differentiation process. This allows controlling its differentiation efficiency into the target cell types. While RNA transcriptomics provides detail at a larger scale, timing and cost are

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    Differentiation of Human Induced Pluripotent Stem Cell into Macrophages
    人诱导多能干细胞向巨噬细胞的分化
    作者:Harriet Douthwaite, Aitor Bermejo Arteagabeitia and Subhankar Mukhopadhyay日期:03/20/2022,浏览量:1259,Q&A: 0
    [Abstract]

    As a model to interrogate human macrophage biology, macrophages differentiated from human induced pluripotent stem cells (hiPSCs) transcend other existing models by circumventing the variability seen in human monocyte-derived macrophages, whilst epitomizing macrophage phenotypic and functional characteristics over those offered by macrophage-like

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    Flow Cytometry Analysis of Planarian Stem Cells Using DNA and Mitochondrial Dyes
    利用DNA和线粒体染料流式细胞术分析涡虫干细胞
    作者:Mohamed Mohamed Haroon, Praveen Kumar Vemula and Dasaradhi Palakodeti日期:01/20/2022,浏览量:1334,Q&A: 0
    [Abstract]

    Planarians are free-living flatworms that emerged as a crucial model system to understand regeneration and stem cell biology. The ability to purify neoblasts, the adult stem cell population of planaria, through fluorescence-activated cell sorting (FACS) has tremendously increased our understanding of pluripotency, specialization, and

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    High-throughput 3D Spheroid Formation and Effective Cardiomyocyte Differentiation from Human iPS Cells Using the Microfabric Vessels EZSPHERETM
    利用微细血管EZSPHERETM从人类iPS细胞中进行高通量三维球状细胞形成和有效的心肌细胞分化
    作者:Tatsuaki Miwa, Alimjan Idiris and Hiromichi Kumagai日期:11/05/2021,浏览量:1410,Q&A: 0
    [Abstract]

    High-throughput 3D spheroid formation from human induced pluripotent stem cells (hiPSCs) can be easily performed using the unique microfabric vessels EZSPHERE, resulting in effective and large scale generation of differentiated cells such as cardiomyocytes or neurons. Such hiPSC-derived cardiomyocytes (hiPSC-CMs) or neurons are very useful in the

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    In situ Hybridization of miRNAs in Human Embryonic Kidney and Human Pluripotent Stem Cell-derived Kidney Organoids
    在人胚肾和人多能干细胞衍生的肾类器官中微小RNA的原位杂交
    作者:Filipa M. Lopes, Susan J. Kimber and Ioannis Bantounas日期:09/05/2021,浏览量:1371,Q&A: 0
    [Abstract]

    MicroRNAs are small RNAs that negatively regulate gene expression and play an important role in fine-tuning molecular pathways during development. There is increasing interest in studying their function in the kidney, but the majority of studies to date use kidney cell lines and assess the total amounts of miRNAs of interest either by qPCR or by

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    CRISPR/Cas9-mediated Precise SNP Editing in Human iPSC Lines
    CRISPR/Cas9介导的人iPSC细胞系SNP精确编辑
    作者:Hanwen Zhang and Siwei Zhang日期:06/20/2021,浏览量:3114,Q&A: 0
    [Abstract]

    Human induced pluripotent stem cells (hiPSCs) have been extensively used in the fields of developmental biology and disease modeling. CRISPR/Cas9 gene editing in iPSC lines often has a low frequency, which hampers its application in precise allele editing of disease-associated single nucleotide polymorphisms (SNPs), especially those in the

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    Generation and Maintenance of Homogeneous Human Midbrain Organoids
    同种人类中脑类器官的生成与维持
    作者:Henrik Renner, Martha Grabos, Hans R. Schöler and Jan M. Bruder日期:06/05/2021,浏览量:3309,Q&A: 0
    [Abstract]

    Three-dimensional cell cultures (“organoids”) promise to better recapitulate native tissue physiology than traditional 2D cultures and are becoming increasingly interesting for disease modeling and compound screening efforts. While a number of protocols for the generation of neural organoids have been published, most protocols require

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    Single or Repeated Ablation of Mouse Olfactory Epithelium by Methimazole
    甲巯咪唑单次或多次消融小鼠嗅上皮细胞
    作者:Sofia Håglin, Staffan Bohm and Anna Berghard日期:04/20/2021,浏览量:3399,Q&A: 0
    [Abstract]

    Odor-detecting olfactory sensory neurons residing in the nasal olfactory epithelium (OE) are the only neurons in direct contact with the external environment. As a result, these neurons are subjected to chemical, physical, and infectious insults, which may be the underlying reason why neurogenesis occurs in the OE of adult mammals. This feature

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    Generation of Human iPSC-derived Neural Progenitor Cells (NPCs) as Drug Discovery Model for Neurological and Mitochondrial Disorders
    人来源iPSC获得的神经祖细胞(NPCs)的产生作为神经和线粒体疾病的药物发现模型
    作者:Annika Zink, Pawel Lisowski and Alessandro Prigione日期:03/05/2021,浏览量:4257,Q&A: 1
    [Abstract]

    The high attrition rate in drug development processes calls for additional human-based model systems. However, in the context of brain disorders, sampling live neuronal cells for compound testing is not applicable. The use of human induced pluripotent stem cells (iPSCs) has revolutionized the field of neuronal disease modeling and drug discovery.

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    Rapid and Simplified Induction of Neural Stem/Progenitor Cells (NSCs/NPCs) and Neurons from Human Induced Pluripotent Stem Cells (hiPSCs)
    从人诱导的多能干细胞(hiPSCs)快速简便地诱导神经干/祖细胞(NSCs/NPCs)和神经元
    作者:Ryutaro Kajihara, Tadahiro Numakawa and Takumi Era日期:02/05/2021,浏览量:3323,Q&A: 0
    [Abstract]

    Human induced pluripotent stem cells (iPSCs) and their progeny displaying tissue-specific characteristics have paved the way for regenerative medicine and research in various fields such as the elucidation of the pathological mechanism of diseases and the discovery of drug candidates. iPSC-derived neurons are particularly valuable as it is

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