植物科学

分类

    现刊
    X-ray Crystallography: Seeding Technique with Cytochrome P450 Reductase
    X射线晶体学:细胞色素P450还原酶加晶种技术
    作者:Bixia Zhang, Jacob A. Lewis, Rishi Hazra and ChulHee Kang日期:11/05/2022,浏览量:262,Q&A: 0
    [Abstract]

    Cytochrome P450 reductase (CPR) is a multi-domain protein that acts as a redox partner of cytochrome P450s. The CPR contains a flavin adenine dinucleotide (FAD)–binding domain, a flavin mononucleotide (FMN)-binding domain, and a connecting domain. To achieve catalytic events, the FMN-binding domain needs to move relative to the FAD-binding

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    Measurement of Ascorbate Peroxidase Activity in Sorghum
    高粱中抗坏血酸过氧化物酶活性的测定
    作者:Praveen Kumar日期:10/20/2022,浏览量:713,Q&A: 0
    [Abstract]

    The ascorbate peroxidase (APX) is a widely distributed antioxidant enzyme. It differs from catalase and other peroxidases in that it scavenges/reduces reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) to water using reduced ascorbate as the electron donor. It is advantageous over other similar antioxidant enzymes in scavenging ROS

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    Collection of Xylem Exudates from the Model Plant Arabidopsis and the Crop Plant Soybean
    从模式植物拟南芥和农作物大豆中收集木质部分泌物
    作者:Satoru Okamoto and Azusa Kawasaki日期:10/05/2022,浏览量:1000,Q&A: 0
    [Abstract]

    A number of molecules, such as secreted peptides, have been shown to mediate root-to-shoot signaling in response to various conditions. The xylem is a pathway for water and molecules that are translocated from roots to shoots. Therefore, collecting and analyzing xylem exudates is an efficient approach to study root-to-shoot long-distance

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    Extraction and Quantification of Plant Hormones and RNA from Pea Axillary Buds
    豌豆腋芽植物激素和 RNA 的提取与定量
    作者:Da Cao, Francois Barbier, Kaori Yoneyama and Christine A. Beveridge日期:10/05/2022,浏览量:737,Q&A: 0
    [Abstract] The quantification of plant hormones and related gene expression is essential to improve the understanding of the molecular regulation of plant growth and development. However, plant hormone quantification is still challenging due to extremely low endogenous levels and high chemical diversity. In this study, we present a convenient extraction ...
    Quantitative Analysis of Redox Pool (NAD+, NADH Content) in Plant Samples Under Aluminum Stress
    铝胁迫下植物样品氧化还原池(NAD+, NADH含量)的定量分析
    作者:Jay Prakash Awasthi, Bedabrata Saha, Hiroyuki Koyama and Sanjib Kumar Panda日期:06/20/2022,浏览量:1613,Q&A: 0
    [Abstract]

    Nicotinamide adenine dinucleotide (NAD) is an essential cofactor of numerous enzymatic reactions found in all living cells. Pyridine nucleotides (NAD+ and NADH) are also key players in signaling through reactive oxygen species (ROS), being crucial in the regulation of both ROS-producing and ROS-consuming systems in plants. NAD content is a

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    Investigation of Transposon DNA Methylation and Copy Number Variation in Plants Using Southern Hybridisation
    利用Southern杂交技术研究植物中转座子DNA甲基化和拷贝数变异的情况
    作者:Vivek Hari Sundar G. and P. V. Shivaprasad日期:06/05/2022,浏览量:1081,Q&A: 0
    [Abstract]

    Plant genomes are pronouncedly enriched in repeat elements such as transposons. These repeats are epigenetically regulated by DNA methylation. Whole genome high-depth sequencing after bisulfite treatment remains an expensive and laborious method to reliably profile the DNA methylome, especially when considering large genomes such as in crops.

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    In vitro Assays to Evaluate Specificity and Affinity in Protein-phospholipid Interactions
    评估蛋白质-磷脂相互作用的特异性和亲和力的体外测定
    [Abstract]

    Protein–lipid interactions play important roles in many biological processes, including metabolism, signaling, and transport; however, computational and structural analyses often fail to predict such interactions, and determining which lipids participate in these interactions remains challenging. In vitro assays to assess the physical

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    Apoplastic Expression of CARD1-ecto Domain in Nicotiana benthamiana and Purification from the Apoplastic Fluids
    本氏烟草中 CARD1-ecto 结构域的质外体表达及质外体液的纯化
    作者:Nobuaki Ishihama, Anuphon Laohavisit, Kaori Takizawa and Ken Shirasu日期:04/20/2022,浏览量:1553,Q&A: 0
    [Abstract]

    The protein expression and purification process is an essential initial step for biochemical analysis of a protein of interest. Traditionally, heterologous protein expression systems (such as E. coli, yeast, insect cells, and cell-free) are employed for plant protein expression, although a plant expression system is often desirable for plant

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    In vitro Auto- and Substrate-Ubiquitination Assays
    体外自体和底物泛素化测定
    作者:Hye Lin Park, Han Yong Lee and Gyeong Mee Yoon日期:04/05/2022,浏览量:1224,Q&A: 1
    [Abstract]

    The precise regulation of the homeostasis of the cellular proteome is critical for the appropriate growth and development of plants. It also allows the plants to respond to various environmental stresses, by modulating their biochemical and physiological aspects in a timely manner. Ubiquitination of cellular proteins is one of the major

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    Activity-based Protein Profiling of Serine Hydrolase Superfamily Enzymes
    丝氨酸水解酶超家族酶的基于活性的蛋白质分析
    作者:Achintya Kumar Dolui, Mahadev Latha and Panneerselvam Vijayaraj日期:03/20/2022,浏览量:1091,Q&A: 0
    [Abstract]

    Activity-based protein profiling (ABPP) is a chemoproteomics platform to assess the functional state of enzymes in complex biological systems. Over the two decades, ABPP has emerged from a gel-based to gel-free platform, for in-depth proteome analysis with enhanced resolution, sensitivity for target detection, and discovery of small molecule

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