细胞生物学

分类

    现刊
    Generation of iMyoblasts from Human Induced Pluripotent Stem Cells
    从人诱导的多能干细胞产生成肌细胞
    作者:Dongsheng Guo, Katelyn Daman, Danielle Fernandes Durso, Jing Yan and Charles P. Emerson Jr.日期:09/05/2022,浏览量:518,Q&A: 0
    [Abstract]

    Skeletal muscle stem cells differentiated from human-induced pluripotent stem cells (hiPSCs) serve as a uniquely promising model system for investigating human myogenesis and disease pathogenesis, and for the development of gene editing and regenerative stem cell therapies. Here, we present an effective and reproducible transgene-free protocol for

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    Preparation of a Single-cell Suspension from Drosophila Wing Imaginal Discs
    果蝇翅成虫芽单细胞悬浮液的制备
    作者:Shu Yang, Brooke Sears and Xiaoyan Zheng日期:08/20/2022,浏览量:823,Q&A: 0
    [Abstract]

    The wing imaginal discs in Drosophila larvae are a pair of sac-like structures that later form the wings of the adult fly. During the past decades, wing discs have been used as a simple and accessible model system, for identifying genes and deciphering signaling cascades that play crucial roles in many aspects of development. In this protocol, we

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    A Fast and Reliable Method to Generate Pure, Single Cell-derived Clones of Mammalian Cells
    一种快速可靠的生成纯的、单细胞衍生的哺乳动物细胞克隆的方法
    作者:Zhe Han, Bindhu K. Madhavan, Serap Kaymak, Peter Nawroth and Varun Kumar日期:08/20/2022,浏览量:1138,Q&A: 0
    [Abstract]

    Stable cell cloning is an essential aspect of biological research. All advanced genome editing tools rely heavily on stable, pure, single cell-derived clones of genetically engineered cells. For years, researchers have depended on single-cell dilutions seeded in 96- or 192-well plates, followed by microscopic exclusion of the wells seeded with

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    A Step-by-step Protocol for Obtaining Mature Microglia from Mice
    从小鼠中获取成熟小胶质细胞的方法
    作者:Min-Jung Yoo and Min-Soo Kwon日期:08/05/2022,浏览量:834,Q&A: 0
    [Abstract]

    In mice, microglial precursors in the yolk sac migrate to the brain parenchyma through the head neuroepithelial layer between embryonic days 8.5 (E8.5)–E16.5 and acquire their unique identity with a ramified form. Based on the microglial developmental process, we dissected the neuroepithelial layer (NEL) of E13.5 mice, which is composed

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    Isolation and ex vivo Expansion of Limbal Mesenchymal Stromal Cells
    边缘间充质基质细胞的分离和离体扩增
    作者:Naresh Polisetti, Lyne Sharaf, Thomas Reinhard and Günther Schlunck日期:07/20/2022,浏览量:697,Q&A: 0
    [Abstract]

    Limbal mesenchymal stromal cells (LMSC), a cellular component of the limbal stem cell niche, have the capability of determining the fate of limbal epithelial progenitor cells (LEPC), which are responsible for the homeostasis of corneal epithelium. However, the isolation of these LMSC has proven to be difficult due to the small fraction of LMSC in

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    A β-glucuronidase (GUS) Based Bacterial Competition Assay to Assess Fine Differences in Fitness during Plant Infection
    基于β-葡萄糖醛酸酶 (GUS) 的细菌竞争测定以评估植物感染期间适应性的细微差异
    作者:Julien S. Luneau, Laurent D. Noël, Emmanuelle Lauber and Alice Boulanger日期:07/05/2022,浏览量:1102,Q&A: 0
    [Abstract]

    Competition assays are a simple phenotyping strategy that confront two bacterial strains to evaluate their relative fitness. Because they are more accurate than single-strain growth assays, competition assays can be used to highlight slight differences that would not otherwise be detectable. In the frame of host-pathogens interactions, they can be

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    Efficient Superovulation and Egg Collection from Mice
    高效的超排卵和小鼠卵子收集
    作者:Miyuki Shindo, Kenji Miyado, Woojin Kang, Maki Fukami and Mami Miyado日期:06/05/2022,浏览量:1000,Q&A: 0
    [Abstract]

    Superovulation is a method used to reduce the number of mice used per experiment by increasing the egg number. Conventionally, superovulation for obtaining mouse eggs involves the use of equine chorionic gonadotropin (eCG) for stimulation and human CG for induction. Female mice of the C57BL/6 inbred strain spontaneously ovulate approximately 10

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    Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat
    分离成年小鼠和大鼠的心肌细胞
    作者:Huiliang Zhang and Peter S. Rabinovitch日期:05/20/2022,浏览量:1347,Q&A: 0
    [Abstract]

    The isolation of intact single adult cardiomyocytes from model animals, mouse and rat, is an essential tool for cardiac molecular and cellular research. While several methods are reported for adult mouse cardiomyocyte isolation, the viability and yield of the isolated cells have been variable. Here, we describe step-by-step protocols for high

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    Immunomagnetic Isolation and Enrichment of Microvascular Endothelial Cells from Human Adipose Tissue
    人体脂肪组织微血管内皮细胞的免疫磁分离和富集
    [Abstract]

    Human adipose tissue-resident microvascular endothelial cells are not only garnering attention for their emergent role in the pathogenesis of obesity-related metabolic disorders, but are also of considerable interest for vascular tissue engineering due, in part, to the abundant, accessible, and uniquely dispensable nature of the tissue. Here, we

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    Isolation of tdTomato Expressing Inter-follicular Epidermal Melanocytes or Keratinocytes from Mouse Tail Skin
    从小鼠尾部皮肤中分离tdTomato表达滤泡间表皮黑色素细胞或角质形成细胞
    作者:Samuel Pop, Oscar Urtatiz and Catherine D. Van Raamsdonk日期:04/20/2022,浏览量:1532,Q&A: 0
    [Abstract]

    The epidermis is the outermost layer of the skin. It is made up of mostly keratinocytes along with a small number of melanocytes and Langerhans cells. Melanocytes produce a pigment called melanin, which is transferred to the keratinocytes, and protects these cells from damage from UV radiation, as well as generating hair and skin colours. In this

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