神经科学

分类

    现刊
    A Simplified Paradigm of Late Gestation Transient Prenatal Hypoxia to Investigate Functional and Structural Outcomes from a Developmental Hypoxic Insult
    妊娠晚期短暂性产前缺氧的简化范式研究发育性低氧损伤的功能和结构结果
    作者:Elyse C. Gadra and Ana G. Cristancho日期:10/05/2022,浏览量:503,Q&A: 0
    [Abstract]

    Late-gestation transient intrauterine hypoxia is a common cause of birth injury. It can lead to long-term neurodevelopmental disabilities even in the absence of gross anatomic injury. Currently, postnatal models of hypoxia–ischemia are most commonly used to study the effect of oxygen deprivation in the fetal brain. These models, however, are

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    A Step-by-step Protocol for Obtaining Mature Microglia from Mice
    从小鼠中获取成熟小胶质细胞的方法
    作者:Min-Jung Yoo and Min-Soo Kwon日期:08/05/2022,浏览量:1082,Q&A: 0
    [Abstract]

    In mice, microglial precursors in the yolk sac migrate to the brain parenchyma through the head neuroepithelial layer between embryonic days 8.5 (E8.5)–E16.5 and acquire their unique identity with a ramified form. Based on the microglial developmental process, we dissected the neuroepithelial layer (NEL) of E13.5 mice, which is composed

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    Generation of a Human Conditionally Immortalized Cell-based Multicellular Spheroidal Blood-Brain Barrier Model for Permeability Evaluation of Macromolecules
    建立用于大分子渗透性评价的人条件永生细胞多细胞球形血脑屏障模型
    作者:Ryuto Isogai, Hanae Morio, Ayaka Okamoto, Keita Kitamura and Tomomi Furihata日期:08/05/2022,浏览量:895,Q&A: 0
    [Abstract]

    There is an urgent need for the development of brain drug delivery carriers based on middle-sized or macromolecules, to which in vitro blood-brain barrier (BBB) models are expected to contribute significantly through evaluation of BBB permeability. As part of efforts to develop such models, we have been working on human conditionally

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    Gene Expression Analysis in Stem Cell-derived Cortical Neuronal Cultures Using Multi-well SYBR Green Quantitative PCR Arrays
    使用多孔 SYBR Green 定量 PCR 阵列在干细胞衍生的皮质神经元培养物中进行基因表达分析
    作者:Vasavi Nallur Srinivasaraghavan, Faria Zafar and Birgitt Schüle日期:07/20/2022,浏览量:1138,Q&A: 0
    [Abstract]

    To optimize differentiation protocols for stem cell-based in vitro modeling applications, it is essential to assess the change in gene expression during the differentiation process. This allows controlling its differentiation efficiency into the target cell types. While RNA transcriptomics provides detail at a larger scale, timing and cost are

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    Identification and Monitoring of Nucleotide Repeat Expansions Using Southern Blotting in Drosophila Models of C9orf72 Motor Neuron Disease and Frontotemporal Dementia
    应用Southern Blotting鉴定和监测C9orf72运动神经元疾病和额颞叶痴呆模型的核苷酸重复扩增
    作者:Joanne L. Sharpe, Nikki S. Harper and Ryan J. H. West日期:05/20/2022,浏览量:942,Q&A: 0
    [Abstract]

    Repeat expansion diseases, including fragile X syndrome, Huntington’s disease, and C9orf72-related motor neuron disease and frontotemporal dementia, are a group of disorders associated with polymorphic expansions of tandem repeat nucleotide sequences. These expansions are highly repetitive and often hundreds to thousands of repeats in

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    High Throughput Blood-brain Barrier Organoid Generation and Assessment of Receptor-Mediated Antibody Transcytosis
    高通量血脑屏障类器官的产生和受体介导的抗体转胞吞作用的评估
    作者:Elena Kassianidou, Claire Simonneau, Alina Gavrilov and Roberto Villaseñor日期:04/20/2022,浏览量:1286,Q&A: 0
    [Abstract]

    Targeting receptor-mediated transcytosis (RMT) is a successful strategy for drug delivery of biologic agents across the blood-brain barrier (BBB). The recent development of human BBB organoid models is a major advancement to help characterize the mechanisms of RMT and thus accelerate the design of brain delivery technologies. BBB organoids exhibit

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    Operant Self-medication for Assessment of Spontaneous Pain Relief and Drug Abuse Liability in Mouse Models of Chronic Pain
    在慢性疼痛小鼠模型中自我用药评估自发性疼痛缓解和药物滥用倾向研究
    作者:David Cabañero, Beltrán Álvarez-Pérez, Elena Martín-García and Rafael Maldonado日期:03/05/2022,浏览量:1069,Q&A: 0
    [Abstract]

    The search for safe and efficient chronic pain treatments is dampened by the lack of reliable models that faithfully reproduce current pharmacological treatments for chronic spontaneous pain in humans. Preclinical models often assess the antinociceptive efficacy of non-contingent pharmacological treatments evaluated in the short-term. Here, we

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    A Novel Standardized Peripheral Nerve Transection Method and a Novel Digital Pressure Sensor Device Construction for Peripheral Nerve Crush Injury
    外周神经挤压伤的一种新型标准化外周神经横切方法及新型数字压力传感器的构建
    作者:Jung Il Lee, Grant D. Wandling, M A Hassan Talukder, Prem Kumar Govindappa and John C. Elfar日期:03/05/2022,浏览量:1038,Q&A: 0
    [Abstract]

    Peripheral nerve injury (PNI) is common in all walks of life, and the most common PNIs are nerve crush and nerve transection. While optimal functional recovery after crush injury occurs over weeks, functional recovery after nerve transection with microsurgical repair and grafting is poor, and associated with permanent disability. The gold-standard

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    Immunoprecipation Assay to Quantify the Amount of tRNAs associated with Their Interacting Proteins in Tissue and Cell Culture
    免疫沉淀法定量组织和细胞培养中与其相互作用蛋白相关的tRNAs的数量
    作者:Sarada Das, Amila Zuko, Robin Thompson, Erik Storkebaum and Zoya Ignatova日期:02/20/2022,浏览量:1850,Q&A: 0
    [Abstract]

    Transfer RNAs (tRNAs) are highly abundant species and, along their biosynthetic and functional path, they establish interactions with a plethora of proteins. The high number of nucleobase modifications in tRNAs renders conventional RNA quantification approaches unsuitable to study protein-tRNA interactions and their associated functional roles in

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    Induction of Repeated Social Defeat Stress in Rats
    大鼠持续性社交失败压力诱导
    作者:Soumyabrata Munshi, Alexandra Ritger and J. Amiel Rosenkranz日期:02/05/2022,浏览量:1312,Q&A: 0
    [Abstract]

    Repeated social defeat stress (RSDS) is a model of chronic stress in rodents. There are several variants of social defeat procedures that exert robust effects in mice, but few published detailed protocols to produce a robust stress and altered immunological profile in rats. In this article, we describe the protocol for the induction of RSDS in

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