细胞生物学

分类

    现刊
    Maximizing the Rod Outer Segment Yield in Retinas Extracted from Cattle Eyes
    最大化从牛眼中提取的视网膜中的杆外段产量
    作者:Isabella Panfoli, Daniela Calzia, Silvia Ravera, Paolo Bianchini and Alberto Diaspro日期:07/20/2022,浏览量:900,Q&A: 0
    [Abstract]

    The retina is a thin neuronal multilayer responsible for the detection of visual information. The first step in visual transduction occurs in the photoreceptor outer segment. The studies on photoreception and visual biochemistry have often utilized rod outer segments (OS) or OS disks purified from mammalian eyes. Literature reports several OS and

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    Whole-mount Senescence-Associated Beta-Galactosidase (SA-β-GAL) Activity Detection Protocol for Adult Zebrafish
    成年斑马鱼的整体衰老相关 β-半乳糖苷酶 (SA-β-GAL) 活性检测方案
    作者:Marta Marzullo, Mounir El Maï and Miguel Godinho Ferreira日期:07/05/2022,浏览量:1924,Q&A: 0
    [Abstract]

    Senescence-associated beta-galactosidase (SA-β-GAL) is an enzyme that accumulates in the lysosomes of senescent cells, where it hydrolyses β-galactosides. With p16, it represents a well-recognized biomarker used to assess senescence both in vivo and in cell culture. The use of a chromogenic substrate, such as

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    Preparation of a Single Cell Suspension from the Murine Iridocorneal Angle
    从小鼠虹膜角膜角制备单细胞悬液
    作者:Benjamin R. Thomson and Susan E. Quaggin日期:05/20/2022,浏览量:1095,Q&A: 0
    [Abstract]

    Single cell RNA sequencing is a powerful tool that can be used to identify distinct cell types and transcriptomic differences within complex tissues. It has proven to be especially useful in tissues of the eye, where investigators have identified novel cell types within the retina, anterior chamber, and iridocorneal angle and explored

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    Ex vivo Human Skin Infection with Herpes Simplex Virus 1
    人皮肤单纯疱疹病毒1的体外感染
    作者:Nydia C. De La Cruz, Maureen Möckel, Lisa Wirtz and Dagmar Knebel-Mörsdorf日期:05/05/2022,浏览量:917,Q&A: 0
    [Abstract]

    Although herpes simplex virus 1 (HSV-1) is a well-studied virus, how the virus invades its human host via skin and mucosa to reach its receptors and initiate infection remains an open question. For studies of HSV-1 infection in skin, mice have been used as animal models. Murine skin infection can be induced after injection or scratching of the

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    Trichloroacetic Acid Fixation and Antibody Staining of Zebrafish Larvae
    斑马鱼幼虫三氯乙酸固定及抗体染色
    作者:E. Anne Martin, Sundas Ijaz, Alberto E. Pereda and Adam C. Miller日期:01/20/2022,浏览量:1678,Q&A: 0
    [Abstract]

    Larval zebrafish have been established as an excellent model for examining vertebrate biology, with many researchers using the system for neuroscience. Controlling a fast escape response of the fish, the Mauthner cells and their associated network are an attractive model, given their experimental accessibility and fast development, driving

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    Whole-mount Immunohistochemistry to Visualize Mouse Embryonic Dermal Lymphatic Vasculature
    小鼠胚胎真皮淋巴血管的免疫组织化学观察
    作者:Xin Geng and R. Sathish Srinivasan日期:10/20/2021,浏览量:1430,Q&A: 0
    [Abstract]

    Lymphatic vessels are abundant in the skin where they regulate interstitial fluid uptake and immune surveillance. Defects in dermal lymphatic vessels, such as fewer vessels and abnormal lymphatic vessel coverage with mural cells, are frequently associated with lymphedema and other lymphatic disorders. Whole-mount immunohistochemistry allows the

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    Phalloidin Staining of Actin Filaments for Visualization of Muscle Fibers in Caenorhabditis elegans
    秀丽隐杆线虫肌动蛋白丝的Phalloidin染色用于肌纤维可视化
    作者:Mario Romani and Johan Auwerx日期:10/05/2021,浏览量:2715,Q&A: 0
    [Abstract]

    Advances in C. elegans research have allowed scientists to recapitulate different human disorders, from neurodegenerative diseases to muscle dysfunction, in these nematodes. Concomitantly, the interest in visualizing organs affected by these conditions has grown, leading to the establishment of different antibody- and dye-based staining protocols

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    Optical Clearing and 3D Analysis Optimized for Mouse and Human Pancreata
    小鼠和人胰腺的光学清除和三维分析优化
    [Abstract]

    The pancreas is a heavily innervated organ, but pancreatic innervation can be challenging to comprehensively assess using conventional histological methods. However, recent advances in whole-mount tissue clearing and 3D rendering techniques have allowed detailed reconstructions of pancreatic innervation. Optical clearing is used to enhance tissue

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    Brain-localized and Intravenous Microinjections in the Larval Zebrafish to Assess Innate Immune Response
    对斑马鱼幼体进行脑局部和静脉显微注射以评估先天免疫反应
    作者:Alison M. Rojas and Celia E. Shiau日期:04/05/2021,浏览量:3974,Q&A: 0
    [Abstract]

    Creating a robust and controlled infection model is imperative for studying the innate immune response. Leveraging the particular strengths of the zebrafish model system, such as optical transparency, ex utero development, and large clutch size, allows for the development of methods that yield consistent and reproducible results. We created a

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    Measuring Bone Volume at Multiple Densities by Micro-computed Tomography
    显微ct测量多重密度骨体积
    作者:Emma C. Walker, Narelle E. McGregor, Audrey S. M. Chan and Natalie A. Sims日期:01/05/2021,浏览量:3049,Q&A: 0
    [Abstract]

    Bone strength is controlled by both bone mass, and the organization and quality of the bone material. The current standard method for measuring bone mass in mouse and rat studies is micro-computed tomography. This method typically uses a single threshold to identify bone material in the cortical and trabecular regions. However, this single

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