植物科学

分类

    现刊
    X-ray Crystallography: Seeding Technique with Cytochrome P450 Reductase
    X射线晶体学:细胞色素P450还原酶加晶种技术
    作者:Bixia Zhang, Jacob A. Lewis, Rishi Hazra and ChulHee Kang日期:11/05/2022,浏览量:262,Q&A: 0
    [Abstract]

    Cytochrome P450 reductase (CPR) is a multi-domain protein that acts as a redox partner of cytochrome P450s. The CPR contains a flavin adenine dinucleotide (FAD)–binding domain, a flavin mononucleotide (FMN)-binding domain, and a connecting domain. To achieve catalytic events, the FMN-binding domain needs to move relative to the FAD-binding

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    Collection of Xylem Exudates from the Model Plant Arabidopsis and the Crop Plant Soybean
    从模式植物拟南芥和农作物大豆中收集木质部分泌物
    作者:Satoru Okamoto and Azusa Kawasaki日期:10/05/2022,浏览量:1000,Q&A: 0
    [Abstract]

    A number of molecules, such as secreted peptides, have been shown to mediate root-to-shoot signaling in response to various conditions. The xylem is a pathway for water and molecules that are translocated from roots to shoots. Therefore, collecting and analyzing xylem exudates is an efficient approach to study root-to-shoot long-distance

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    In vitro Assays to Evaluate Specificity and Affinity in Protein-phospholipid Interactions
    评估蛋白质-磷脂相互作用的特异性和亲和力的体外测定
    [Abstract]

    Protein–lipid interactions play important roles in many biological processes, including metabolism, signaling, and transport; however, computational and structural analyses often fail to predict such interactions, and determining which lipids participate in these interactions remains challenging. In vitro assays to assess the physical

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    Apoplastic Expression of CARD1-ecto Domain in Nicotiana benthamiana and Purification from the Apoplastic Fluids
    本氏烟草中 CARD1-ecto 结构域的质外体表达及质外体液的纯化
    作者:Nobuaki Ishihama, Anuphon Laohavisit, Kaori Takizawa and Ken Shirasu日期:04/20/2022,浏览量:1553,Q&A: 0
    [Abstract]

    The protein expression and purification process is an essential initial step for biochemical analysis of a protein of interest. Traditionally, heterologous protein expression systems (such as E. coli, yeast, insect cells, and cell-free) are employed for plant protein expression, although a plant expression system is often desirable for plant

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    In vitro Auto- and Substrate-Ubiquitination Assays
    体外自体和底物泛素化测定
    作者:Hye Lin Park, Han Yong Lee and Gyeong Mee Yoon日期:04/05/2022,浏览量:1224,Q&A: 1
    [Abstract]

    The precise regulation of the homeostasis of the cellular proteome is critical for the appropriate growth and development of plants. It also allows the plants to respond to various environmental stresses, by modulating their biochemical and physiological aspects in a timely manner. Ubiquitination of cellular proteins is one of the major

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    Activity-based Protein Profiling of Serine Hydrolase Superfamily Enzymes
    丝氨酸水解酶超家族酶的基于活性的蛋白质分析
    作者:Achintya Kumar Dolui, Mahadev Latha and Panneerselvam Vijayaraj日期:03/20/2022,浏览量:1091,Q&A: 0
    [Abstract]

    Activity-based protein profiling (ABPP) is a chemoproteomics platform to assess the functional state of enzymes in complex biological systems. Over the two decades, ABPP has emerged from a gel-based to gel-free platform, for in-depth proteome analysis with enhanced resolution, sensitivity for target detection, and discovery of small molecule

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    An in vitro Coupled Assay for PEPC with Control of Bicarbonate Concentration
    一种控制碳酸氢盐浓度的磷酸烯醇式丙酮酸羧化酶的体外偶联分析
    作者:Nicholas R. Moody, Chatawal Phansopal and James D. Reid日期:12/20/2021,浏览量:1291,Q&A: 0
    [Abstract]

    Phosphoenolpyruvate carboxylase (PEPC) catalyzes a critical step in carbon metabolism in plants and bacteria, the irreversible reaction between bicarbonate and phosphoenolpyruvate to produce the C4 compound oxaloacetate. This enzyme is particularly important in the context of C4 photosynthesis, where it is the initial carbon-fixing enzyme. Many

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    High Throughput Analyses of Ascorbate-turnover Enzyme Activities in Rice (Oryza sativa L.) Seedlings
    水稻幼苗抗坏血酸转化酶活性的高通量分析
    作者:Lin-Bo Wu, Yanru Feng, Frederike Zeibig, Muhammad Shahedul Alam and Michael Frei日期:10/20/2021,浏览量:1830,Q&A: 0
    [Abstract]

    Ascorbate (Vitamin C) fulfills various functions in plant photosynthesis and abiotic stress tolerance. The four key enzymes involved in the ascorbate-turnover pathway are ascorbate peroxidase, ascorbate oxidase, monodehydroascorbate reductase, and dehydroascorbate reductase. Several reports have shown the pivotal roles of these enzymes in plant

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    Proteoliposomes for Studying Lipid-protein Interactions in Membranes in vitro
    体外研究膜脂-蛋白相互作用的蛋白质脂质体
    作者:Helmut Kirchhoff日期:10/20/2021,浏览量:1426,Q&A: 0
    [Abstract]

    Lipids in biomembranes can control the structure and, therefore, the functionality of membrane-embedded protein complexes. Unraveling how the lipid composition determines the mode of operation of membrane proteins provides mechanistic insights into their functionality. We applied a proteoliposome technique for studying how proteins function in

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    Solubilization Method for Isolation of Photosynthetic Mega- and Super-complexes from Conifer Thylakoids
    从针叶树类囊体中分离光合巨复合体和超复合体的增溶方法
    作者:Pushan Bag, Wolfgang P. Schröder, Stefan Jansson and Domenica Farci日期:09/05/2021,浏览量:1954,Q&A: 0
    [Abstract]

    Photosynthesis is the main process by which sunlight is harvested and converted into chemical energy and has been a focal point of fundamental research in plant biology for decades. In higher plants, the process takes place in the thylakoid membranes where the two photosystems (PSI and PSII) are located. In the past few decades, the evolution of

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