细胞生物学

分类

    现刊
    Generation of iMyoblasts from Human Induced Pluripotent Stem Cells
    从人诱导的多能干细胞产生成肌细胞
    作者:Dongsheng Guo, Katelyn Daman, Danielle Fernandes Durso, Jing Yan and Charles P. Emerson Jr.日期:09/05/2022,浏览量:279,Q&A: 0
    [Abstract]

    Skeletal muscle stem cells differentiated from human-induced pluripotent stem cells (hiPSCs) serve as a uniquely promising model system for investigating human myogenesis and disease pathogenesis, and for the development of gene editing and regenerative stem cell therapies. Here, we present an effective and reproducible transgene-free protocol for

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    A Step-by-step Protocol for Obtaining Mature Microglia from Mice
    从小鼠中获取成熟小胶质细胞的方法
    作者:Min-Jung Yoo and Min-Soo Kwon日期:08/05/2022,浏览量:782,Q&A: 0
    [Abstract]

    In mice, microglial precursors in the yolk sac migrate to the brain parenchyma through the head neuroepithelial layer between embryonic days 8.5 (E8.5)–E16.5 and acquire their unique identity with a ramified form. Based on the microglial developmental process, we dissected the neuroepithelial layer (NEL) of E13.5 mice, which is composed

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    Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat
    分离成年小鼠和大鼠的心肌细胞
    作者:Huiliang Zhang and Peter S. Rabinovitch日期:05/20/2022,浏览量:1305,Q&A: 0
    [Abstract]

    The isolation of intact single adult cardiomyocytes from model animals, mouse and rat, is an essential tool for cardiac molecular and cellular research. While several methods are reported for adult mouse cardiomyocyte isolation, the viability and yield of the isolated cells have been variable. Here, we describe step-by-step protocols for high

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    Isolation and Culture of Cranial Neural Crest Cells from the First Branchial Arch of Mice
    小鼠第一鳃弓颅神经嵴细胞的分离培养
    作者:Hiroki Ueharu, Jingwen Yang, Yoshihiro Komatsu and Yuji Mishina日期:04/05/2022,浏览量:966,Q&A: 1
    [Abstract]

    Craniofacial anomalies (CFA) are a diverse group of deformities, which affect the growth of the head and face. Dysregulation of cranial neural crest cell (NCC) migration, proliferation, differentiation, and/or cell fate specification have been reported to contribute to CFA. Understanding of the mechanisms through which cranial NCCs contribute

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    Differentiation of Human Induced Pluripotent Stem Cell into Macrophages
    人诱导多能干细胞向巨噬细胞的分化
    作者:Harriet Douthwaite, Aitor Bermejo Arteagabeitia and Subhankar Mukhopadhyay日期:03/20/2022,浏览量:1411,Q&A: 0
    [Abstract]

    As a model to interrogate human macrophage biology, macrophages differentiated from human induced pluripotent stem cells (hiPSCs) transcend other existing models by circumventing the variability seen in human monocyte-derived macrophages, whilst epitomizing macrophage phenotypic and functional characteristics over those offered by macrophage-like

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    Efficient Method to Differentiate Mouse Embryonic Stem Cells into Macrophages in vitro
    将小鼠胚胎干细胞体外分化为巨噬细胞的有效方法
    作者:Qimin Hai, Juying Han, Sophia Wells and Jonathan D. Smith日期:02/05/2022,浏览量:1741,Q&A: 0
    [Abstract]

    Macrophages are key cells in the innate immune system and play a role in a variety of diseases. However, macrophages are terminally differentiated and difficult to manipulate genetically via transfection or through CRISPR-Cas9 gene editing. To overcome this limitation, we provide a simplified protocol for the generation of mouse embryonic stem

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    A Method to Induce Brown/Beige Adipocyte Differentiation from Murine Preadipocytes
    一种从鼠前脂肪细胞诱导棕色/米色脂肪细胞分化的方法
    作者:Andréa Livia Rocha, Beatriz Alves Guerra, Jeremie Boucher and Marcelo A. Mori日期:12/20/2021,浏览量:1866,Q&A: 0
    [Abstract]

    Adipocytes exhibit different morphological and functional characteristics, depending on their anatomical location, developmental origin, and stimulus. While white adipocytes tend to accumulate energy as triglycerides, brown and beige adipocytes tend to direct carbon sources to fuel thermogenesis. White and beige adipocytes originate from common

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    High-throughput 3D Spheroid Formation and Effective Cardiomyocyte Differentiation from Human iPS Cells Using the Microfabric Vessels EZSPHERETM
    利用微细血管EZSPHERETM从人类iPS细胞中进行高通量三维球状细胞形成和有效的心肌细胞分化
    作者:Tatsuaki Miwa, Alimjan Idiris and Hiromichi Kumagai日期:11/05/2021,浏览量:1536,Q&A: 0
    [Abstract]

    High-throughput 3D spheroid formation from human induced pluripotent stem cells (hiPSCs) can be easily performed using the unique microfabric vessels EZSPHERE, resulting in effective and large scale generation of differentiated cells such as cardiomyocytes or neurons. Such hiPSC-derived cardiomyocytes (hiPSC-CMs) or neurons are very useful in the

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    Mouse Periosteal Cell Culture, in vitro Differentiation, and in vivo Transplantation in Tibial Fractures
    小鼠骨膜细胞培养、体外分化及胫骨骨折的体内移植
    作者:Simon Perrin, Anais Julien, Oriane Duchamp de Lageneste, Rana Abou-Khalil and Céline Colnot日期:08/05/2021,浏览量:1958,Q&A: 0
    [Abstract]

    The periosteum covering the outer surface of bone contains skeletal stem/progenitor cells that can efficiently form cartilage and bone during bone repair. Several methods have been described to isolate periosteal cells based on bone scraping and/or enzymatic digestion. Here, we describe an explant culture method to isolate periosteum-derived

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    Mechanical Fractionation of Cultured Neuronal Cells into Cell Body and Neurite Fractions
    培养神经元细胞成细胞体和神经突部分的机械分离
    作者:Ankita Arora, Raeann Goering, Hei-Yong G. Lo and J. Matthew Taliaferro日期:06/05/2021,浏览量:3124,Q&A: 0
    [Abstract]

    Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The subcellular distribution of these localized proteins is often facilitated by the subcellular localization of the RNA molecules that encode them. A key question in the study of this process of RNA localization is the

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