0 Q&A
9050 Views
Jan 5, 2013
This protocol describes the affinity purification of the chloroplast ClpP complex from Chlamydomonas reinhardtii. To this purpose, we have created a Chlamydomonas reinhardtii strain in which the chloroplast encoded ClpP1 subunit of the ClpP complex is tagged at its C-terminal end by a strep-tagII peptide.