癌症生物学

分类

    现刊
    Detection of Alternative End-Joining in HNSC Cell Lines Using DNA Double-Strand Break Reporter Assays
    使用 DNA 双链断裂报告基因检测HNSC细胞系中的替代末端连接
    作者:Nan Zuo, Lin Ma, Weitao Hu, Yongqiang Deng, Lanlan Wei and Qi Liu日期:09/05/2022,浏览量:687,Q&A: 0
    [Abstract]

    The main cellular pathways to repair DNA double-strand breaks (DSBs) and protect the integrity of the genome are homologous recombination (HR), non-homologous end-joining (NHEJ), and alternative end-joining (Alt-EJ). Polymerase theta-regulated Alt-EJ is an error-prone DSB repair pathway characterized by microhomology usage. Considering its

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    Conditional Human BRD4 Knock-In Transgenic Mouse Genotyping and Protein Isoform Detection
    条件性人 BRD4 敲入转基因小鼠基因分型和蛋白质异构体检测
    作者:Michael Paul Lewis, Shwu-Yuan Wu and Cheng-Ming Chiang日期:04/05/2022,浏览量:877,Q&A: 0
    [Abstract]

    Bromodomain-containing protein 4 (BRD4) is an acetyl-lysine reader protein and transcriptional regulator implicated in chromatin dynamics and cancer development. Several BRD4 isoforms have been detected in humans with the long isoform (BRD4-L, aa 1-1,362) playing a tumor-suppressive role and a major short isoform (BRD4-S, aa 1-722) having

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    Enhanced-ice-COLD-PCR for the Sensitive Detection of Rare DNA Methylation Patterns in Liquid Biopsies
    Enhanced-ice-COLD-PCR用于液体活检中罕见DNA甲基化模式的敏感检测
    作者:Florence Mauger and Jörg Tost日期:12/05/2019,浏览量:4157,Q&A: 0
    [Abstract] In the context of precision medicine, the identification of novel biomarkers for the diagnosis of disease, prognosis, predicting treatment outcome and monitoring of treatment success is of great importance. The analysis of methylated circulating-cell free DNA provides great promise to complement or replace genetic markers for these applications, ...
    Centromere Chromosome Orientation Fluorescent in situ Hybridization (Cen-CO-FISH) Detects Sister Chromatid Exchange at the Centromere in Human Cells
    着丝粒染色体定位荧光原位杂交法(Cen-CO-FISH)检测人细胞中着丝粒DNA重复的姐妹染色单体交换事件
    作者:Simona Giunta日期:04/05/2018,浏览量:7343,Q&A: 0
    [Abstract] Human centromeres are composed of large tandem arrays of repetitive alpha satellite DNA, which are often sites of aberrant rearrangement in cancers (Mitelman et al., 1997; Padilla-Nash et al., 2001). To date, annotation of the human centromere repetitive sequences remains incomplete, greatly hindering in-depth functional studies ...