生物化学

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    现刊
    A Robust, One-step FRET Assay for Human Heparanase
    人乙酰肝素酶的一步快速FRET检测
    作者:Jyothi C. Sistla and Umesh R. Desai日期:09/05/2019,浏览量:3013,Q&A: 0
    [Abstract] Heparanase, an endo-β-D-glucuronidase, cleaves cell surface and extracellular matrix heparan sulfate (HS) chains at distinct sites and plays important biological roles including modulation of cell growth and metastasis. Although a number of different types of heparanase assays have been reported to date, most are labor intensive, complex ...
    [2-3H]Mannose-labeling and Analysis of N-linked Oligosaccharides
    N-连接寡糖的[2-3H] 甘露糖标记和分析
    作者:Marina Shenkman, Navit Ogen-Shtern and Gerardo Z. Lederkremer日期:07/20/2017,浏览量:6115,Q&A: 0
    [Abstract] Modifications of N-linked oligosaccharides of glycoproteins soon after their biosynthesis correlate to glycoprotein folding status. These alterations can be detected in a sensitive way by pulse-chase analysis of [2-3H]mannose-labeled glycoproteins, with enzymatic removal of labeled N-glycans, separation according to size by HPLC and ...