病毒

分类

    现刊
    Production of Recombinant Hepatitis B virus (HBV) and Detection of HBV in Infected Human Liver Organoids
    重组乙型肝炎病毒 (HBV) 的产生和受感染的人类肝脏类器官中 HBV 的检测
    作者:Tanvir Hossain, Shahla Romal and Tokameh Mahmoudi日期:04/20/2022,浏览量:543,Q&A: 0
    [Abstract]

    The absence of long term, primary untransformed in vitro models that support hepatitis B virus (HBV) infection and replication have hampered HBV pre-clinical research, which was reflected in the absence of a curative therapy until recently. One of the limitations for in vitro HBV research has been the absence of high titer and pure recombinant HBV

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    Generation, Maintenance and HBV Infection of Human Liver Organoids
    人肝类器官的产生、维持和HBV感染
    作者:Shahla Romal, Tanvir Hossain and Tokameh Mahmoudi日期:03/20/2022,浏览量:1119,Q&A: 0
    [Abstract]

    Hepatitis B virus (HBV) infection represents a major public health problem infecting approximately 400 million people worldwide. Despite the availability of a preventive vaccine and anti-viral therapies, chronic HBV infection remains a major health issue because it increases the risk of developing liver cirrhosis and hepatocellular carcinoma

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    Quantification of Hepatitis B Virus Covalently Closed Circular DNA in Infected Cell Culture Models by Quantitative PCR
    qPCR定量分析感染细胞培养模型中乙型肝炎病毒共价闭合环状DNA
    作者:Bingqian Qu and Stephan Urban日期:04/05/2019,浏览量:5538,Q&A: 0
    [Abstract] Persistence of the human hepatitis B virus (HBV) requires the maintenance of covalently closed circular (ccc)DNA, the episomal genome reservoir in nuclei of infected hepatocytes. cccDNA elimination is a major aim in future curative therapies currently under development. In cell culture based in vitro studies, both hybridization- and ...
    Sleeping Beauty Transposon-based System for Rapid Generation of HBV-replicating Stable Cell Lines
    使用基于睡美人转座子的系统快速生成HBV复制稳定细胞系
    作者:Jin-Wei Zheng, Jia-Li Cao and Quan Yuan 日期:07/05/2018,浏览量:7102,Q&A: 0
    [Abstract] The stable HBV-transfected cell lines, which based on stable integration of replication-competent HBV genome into hepatic cells, are widely used in basic research and antiviral drug evaluation against HBV. However, previous reported strategies to generate HBV-replicating cell lines, which primarily rely on random integration of exogenous DNA by ...
    In vitro Drug Susceptibility Assay for HBV Using Southern Blotting
    采用Southern蛋白印迹法进行HBV体外药敏性试验
    作者:Sung Hyun Ahn, Yong Kwang Park and Kyun-Hwan Kim日期:04/20/2015,浏览量:9836,Q&A: 0
    [Abstract] Antiviral agents for the suppression of hepatitis B virus (HBV) have been used for treating chronic hepatitis B. However, the emergence of drug-resistant HBV is still a major problem for antiviral treatment. To identify and characterize the drug-resistant HBV, the construction of HBV replicon and in vitro drug susceptibility assay are ...